1887

Abstract

The entire envelope gene of human T cell leukaemia virus type I (HTLV-I) has been successfully expressed in a baculovirus non-fusion vector system. The HTLV-I envelope protein accumulated within the insect cells as inclusion bodies which allowed efficient recovery of the recombinant protein. In an attempt to study the role of the HTLV-I envelope glycoprotein as an immunogenic target, mice were immunized with the envelope protein inclusion bodies (env-I.B.) in the presence or absence of an adjuvant. Antibodies of broad specificity were produced against the HTLV-I envelope protein in the presence or absence of an adjuvant as detected by Western blotting, radioimmunoprecipitation and peptide ELISA. Neutralizing antibody was detected when env-I.B. immunizations were carried out in the presence of high doses of a new adjuvant composed of a mycobacterial cell wall extract. In a combined immunization regimen, env-I.B. were found to enhance and broaden the antibody response to the HTLV-I envelope glycoprotein, following priming with various recombinant vaccinia virus (RVV) constructs expressing either the entire native HTLV-I envelope (gp46 and gp21) or just the surface envelope protein (gp46). Increased titres of neutralizing antibodies were observed following priming with the RVV expressing gp46 only. Results indicate that immunization regimes that involve priming with RVV expressing HTLV-I envelope followed by boosting with recombinant baculoviral HTLV-I envelope might be useful in eliciting protective immune responses .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-2-211
1993-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/2/JV0740020211.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-2-211&mimeType=html&fmt=ahah

References

  1. Archambault G. 1989; Effect of sodium diethyldithiocarbamate, Corynebacterium parvum and mycobacterial cell wall extract on in vitro blastogenic responses of bovine blood lymphocytes. Cornell Veterinarian 79:11–24
    [Google Scholar]
  2. Ball J. K., McCarter J. A. 1979; Biological characterization of a leukemogenic virus isolated from the CFW mouse. Cancer Research 39:3080–3088
    [Google Scholar]
  3. Blattner W., Nomura A., Clark J., Ho G., Nakao Y., Gallo R., Robert-Guroff M. 1986; Modes of transmission and evidence for viral latency from studies of human T-cell lymphotrophic virus type I in Japanese migrant populations in Hawaii. Proceedings of the National Academy of Sciences, U.S.A 834895–4898
    [Google Scholar]
  4. Bonifacino J. S., Cosson P., Shah N., Klausner R. D. 1991; Role of potentially charged transmembrane residues in targeting proteins for retention and degradation within the endoplasmic reticulum. EMBO Journal 10:2783–2793
    [Google Scholar]
  5. Chen Y. M. A., Lee T. H., Samuel K. P., Okayama A., Tachibana, N-, Miyoshi I., Papas T. S., Essex M. 1989; Antibody reactivity to different regions of human T-cell leukemia virus type I gp61 in infected people. Journal of Virology 63:4952–4957
    [Google Scholar]
  6. Clapham P., Nagy K., Weiss R. A. 1984; Pseudotypes of human T-cell leukemia virus type 1 and 2: tieutralization by patients' sera. Proceedings of the National Academy of Sciences, U.S.A 812886–2889
    [Google Scholar]
  7. Copeland T. D., Tsai W.-P., Kim Y. D., Oroszlan S. 1986; Envelope proteins of human T-cell leukemia virus type I: characterization of antisera to synthetic peptides and identification of a natural epitope. Journal of Immunology 137:2945–2951
    [Google Scholar]
  8. Dekaban G. A., Ball J. K., Robey W., G Arthur L. O., McCarter J. A. 1984; Molecular biological characterizationof a highly leukaemogenic virus isolated from the mouse. IV. Viral proteins. Journal of General Virology 65:1791–1802
    [Google Scholar]
  9. Dickson C., Eisenman R., Fan F., Hunter E., Teich N. 1982; Protein biosynthesis and assembly. In RNA Tumor Viruses pp 513–646 Edited by Weiss R. A., Teich N., Varmus H., Coffin J. New York: Cold spring Harbor Laboratory;
    [Google Scholar]
  10. Ford C., Arp J., King E., Dekaban G. A., Palker T. 1991; Expression and immunogenicity of HTLV-I envelope proteins by recombinant vaccinia virus. In Vaccines 91: Modern Approaches to New Vaccines pp 253–258 Edited by Chanock R. M-, Ginsberg H- S., Brown F. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Ford C. M., Arp J., Palker T. J., King E.E., Dekaban G. A. 1992; Characterization of the antibody response to three different versions of the HTLV-1 envelope protein expressed by recombinant vaccinia viruses: induction of neutralizing antibody. Virology 191:448–453
    [Google Scholar]
  12. Hayden D., B Baker N. R., Percival M. P., Beckwith P. B. 1986; Modification of the photosystem II light-harvesting chlorophyll a/b protein complex in maize during chill-induced photoinhibition. Biochimica et biophysica acta 851:86–92
    [Google Scholar]
  13. Horal P., Hall W. W., Svennerholm B., Lycke J., Jeansson S., Rymo L., Kaplan M. H., Vahlne A. 1991; Identification of type-specific Unear epitopes in the glycoproteins gp46 and gp21 of human T-cell leukemia viruses type I and type II using synthetic peptides. Proceedings of the National Academy of Sciences, U.S.A 885754–5758
    [Google Scholar]
  14. Hu S., L-, Kosowski S. G., Schaap K. F. 1987; Expression of envelope glycoproteins of human immunodeficiency virus by an insect virus vector. Journal of Virology 61:3617–3620
    [Google Scholar]
  15. Jacobson S., Reuben J., Streilein R., Palker T. 1991; Induction of CD4+, HTLV-I specific cytotoxic T-lymphocytes from patients with HAM-TSP: recognition of an immunogenic region of the gp46 envelope glycoprotein of HTLV-I. Journal of Immunology 146:1155–1162
    [Google Scholar]
  16. Johnson D. A., Gautsch J., W Sportsman J. R., Elder J. H. 1984; Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Genetic Analysis Techniques 1:3–8
    [Google Scholar]
  17. Kieny M., Lathe R., Riviere Y., Dott K., Schmitt D., Girard M-, Montagnier L., Lecocq J.-P. 1988; Improved antigenicity of the HIV env protein by cleavage site removal. Protein Engineering 2:219–225
    [Google Scholar]
  18. Kinoshita K., Amagasaki T., Hino S., Doi H-, Yamanouchi K., Ban N., Momita S., Ikeda S., Kamihira S., Ichimaru M-, Katamine S., Miyamoto T., Tsuji Y., Ishimaru T., Yamabe T., Ito M-, Kamura S., Tsuda T. 1987; Milk-borne transmission of HTLV-I from carrier mothers to their children. Japanese Journal of Cancer Research 78:674–680
    [Google Scholar]
  19. Kiyokawa T., Yoshikura H., Hattori S., Seiki M., Yoshida M. 1984; Envelope proteins of human T-cell leukemia virus; expression in Escherichia coll and its application to studies of env gene functions. Proceedings of the National Academy of Sciences, U.S.A 816202–6206
    [Google Scholar]
  20. Kuga T., Hattori S., Yoshida M., Taniguchi T. 1986; Expression of human T-cell leukemia virus type I envelope protein in Saccharomyces cerevisiae. Gene 44:337–340
    [Google Scholar]
  21. Kurata A., Palker T., Streilein R., Scearce R., Haynes B., Berzofsky J. 1989; Immunodominant sites on human T-cell leukemia virus type 1 envelope protein for murine T-cells. Journal of Immunology 143:2024–2030
    [Google Scholar]
  22. Kuroda K., Hauser C., Rott R., Klenk H., Doerfler W. 1986; Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. EMBO Journal 5:1359–1365
    [Google Scholar]
  23. Lal R. B., Rudolph D. L., Palker T. J., Coligan J. E., Folks T. M. 1991; A synthetic peptide elicits antibody reactive with the external envelope glycoprotein of human T cell lymphotropic virus type I. Journal of General Virology 72:2321–2324
    [Google Scholar]
  24. Li Y., Luo L., Rassol N., Kang C. Y. 1992; Essence of human immunodeficiency virus gpl20 folding for CD4 binding. Journal of Virology (in press)
    [Google Scholar]
  25. Miller L. K. 1988; Baculoviruses as gene expression vectors. Annual Review of Microbiology 42:177–199
    [Google Scholar]
  26. Nagy K., Clapham P., Cheingsong-Popov R., Weiss R. A. 1983; Human leukemia virus type I: induction of syncytia and inhibition by patients’ sera. International Journal of Cancer 32:321–328
    [Google Scholar]
  27. Nakamura H., Hayami M., Ohta Y., Ishikawa K., Tsuijimoto H., Kiyokawa T., Yoshida M., Sasagawa A., Honjo S. 1987; Protection of cynomolgus monkeys against infection by human T-cell leukemia virus type-I by immunization with viral env gene products produced in Escherichia coli. International Journal of Cancer 40:403–407
    [Google Scholar]
  28. Nyunoya H., Ogura T., Kikuchi M., Iwamoto H., Yamashita K., Maekawa M., Takebe Y., Miyamura K., Yamazaki S., Shimotohno K. 1990; Expression of HTLV-I envelope protein to hydrophobic amino-terminal peptide of baculovirus polyhedrin in insect cells and its application for serological assays. AIDS Research and Human Retroviruses 6:1311–1321
    [Google Scholar]
  29. Osame M., Usuku K., Izumo S., Ijichi N., Amitani H., Igata A., Matsumoto M., Tara M. 1986; HTLV-I-associated myelopathy, a new clinical entity. Lancet i:1031–1032
    [Google Scholar]
  30. Palker T., Tanner M., Scearce R., Streilein R., Clark M., Haynes B. 1989; ¦ Mapping of immunogenic regions of human T-cell leukemia type I (HTLV-I) gp46 and gp21 envelope glycoproteins with env-encoded synthetic peptides and a monoclonal antibody to gp46. Journal of Immunology 142:971–978
    [Google Scholar]
  31. Palker T. J., Riggs E. R., Spragion D. E., Muir A. J., Scearce R. M., Randall R. R., McAdams M. W., McKnight A., Clapham P. R., Weiss R. A., Haynes B. F. 1992; Mapping of homologous, amino-terminal neutralizing regions of human T-cell lymphotropic virus type I and II gp46 envelope glycoproteins. Journal of Virology 66:5879–5889
    [Google Scholar]
  32. Pique C., Tursz T., Dokhelar M.-C. 1990; Mutations introduced along the HTLV-I envelope gene result in a non-functional protein: a basis for envelope conservation?. EMBO Journal 9:4243–4248
    [Google Scholar]
  33. Pique C., Pham D., Tursz T., Dokhelar M. C. 1992; Human T-cell leukemia virus type I envelope protein maturation process: requirements for syncytium formation. Journal of Virology 66:906–913
    [Google Scholar]
  34. Ratner L., Josephs S. F., Starcich B., Hahn B., Shaw G. M., Gallo R. C., Wong-Staal F. 1985; Nucleotide sequence analysis of a variant human T-cell leukemia virus (HTLV-Ib) provirus with a deletion in pX-I. Journal of Virology 54:781–790
    [Google Scholar]
  35. Robert-Guroff M., Nakao Y., Notake K., Ito Y., Sliski A., Gallo R. C. 1982; Natural antibodies to human retrovirus HTLV in a cluster of Japanese patients with adult T cell leukemia. Science 215:975–978
    [Google Scholar]
  36. Ruseche J. R., Lynn. D. L., Robert-Guroff M., Langlois A. J., Lyerly H. K., Carson H., Krohn K., Ranki A., Gallo R. C., Bolognesi D. P., Putney S. D., Matthews T. J. 1987; Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells. Proceedings of the National Academy of Sciences, U.S.A 846924–6928
    [Google Scholar]
  37. Samuel K. P., Lautenberger J. A., Jorcyk C. L., Josephs S., Wong-Staal F., Papas T. S. 1984; Diagnostic potential for human malignancies of bacterially produced HTLV-I envelope glycoprotein. Science 226:1094–1097
    [Google Scholar]
  38. Satow Y., Hashido M., Ishikawa K., Honds H., Mizuno M., Kawana T., Hayami M. 1991; Detection of HTLV-I antigen in peripheral and cord blood lymphocytes from carrier mothers. Lancet 338:915–916
    [Google Scholar]
  39. Shida H., Tochikura T., Sato T., Konno T., Hirayoshi K., Seki M., Ito Y., Hatanaka M., Hinuma M., Sugimoto M., Takahashi-Nishimaki F., Manama T., Miki K., Suzuki K., Morita H., Sashiyama H., Hayami M. 1987; Effect of recombinant vaccinia viruses that express HTLV-I envelope gene on HTLV-I infection. EMBO Journal 6:3379–3384
    [Google Scholar]
  40. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin no. 1555
    [Google Scholar]
  41. Tanaka Y., Zeng L., Shiraki H., Shida H., Tozawa H. 1991; Identification of a neutralization epitope on the envelope gp46 antigen of human T cell leukemia virus type I and induction of neutralizing antibody by peptide immunization. Journal of Immunology 147:354–360
    [Google Scholar]
  42. Teyton L., O’Sullivan D., Dickson P. W., Lotteau V., Sette A., Fink P., Peterson P. A. 1990; Invariant chain distinguishes between the exogenous and endogenous antigen presentation pathways. Nature, London 348:39–44
    [Google Scholar]
  43. Vile R. G., Schulz T. F., Danos O. F., Collins M. K. L., Weiss R. A. 1991; A murine cell line producing HTLV-I pseudotype virions carrying a selectable marker gene. Virology 180:420–424
    [Google Scholar]
  44. Yoshida M., Seiki M., Yamaguchi K., Takatsuki K. 1984; Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. Proceedings of the National Academy of Sciences, U.S.A 812534–2537
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-2-211
Loading
/content/journal/jgv/10.1099/0022-1317-74-2-211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error