1887

Abstract

The nucleotide sequences of the nucleoprotein (N) genes of seven tospovirus isolates representing three serogroups were determined and used to establish phylogenetic parameters to delineate species within the genus of the Bunyaviridae. A high sequence divergence (55.9% identity at the nucleotide level) was observed between isolates of serogroup I (tomato spotted wilt virus) and isolates of serogroup III ( necrotic spot virus). The serogroup II isolates take an intermediate position. Their N genes have 75% identity with those of serogroup I isolates and 57% with those of serogroup III isolates. Whereas the isolates within serogroups I or III have almost identical sequences, the two isolates BR-03 and SA-05 of serogroup II diverged significantly from each other (82·1% sequence identity). The results obtained support the conclusion that, in addition to the species TSWV and INSV, the serogroup II isolates BR-03 and SA-05 have to be considered as distinct species within the genus for which the names tomato chlorotic spot virus and groundnut ringspot virus, respectively, are proposed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-2-153
1993-02-01
2022-08-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/2/JV0740020153.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-2-153&mimeType=html&fmt=ahah

References

  1. Arikawa J., Lapenotiere H. F., Iacono-Connors L., Wang M., Schmaljohn C. S. 1990; Coding properties of the S and the M genome segments of Sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
    [Google Scholar]
  2. Beaton A. R., Kkug R. M. 1984; Synthesis of the templates for influenza virion RNA replication in vitro. Proceedings of the National Academy of Sciences, U.S.A 814682–4686
    [Google Scholar]
  3. Beaton A. R., Krug R. M. 1986; Transcription, antitermination during influenza viral template RNA synthesis requires the nucleocapsid protein and the absence of a 5' capped end. Proceedings of the National Academy of Sciences, U.S.A 836282–6286
    [Google Scholar]
  4. Chanekar A. M., Reddy D. V. R., Iizuka N., Amin P. W., Gibbous R. W. 1979; Bud necrosis of groundnut (Arachis hypogaea) in India caused by tomato spotted wilt virus. Annals of Applied Biology173–179
    [Google Scholar]
  5. Cho J. J. 1986; Reservoir weed hosts of tomato spotted wilt virus. Plant Disease 70:1014–1016
    [Google Scholar]
  6. de Ávila A. C., Huguenot C., Resende R. de O., Kitajima E. W., Goldbach R. W., Peters D. 1990; Serological differentiation of 20 isolates of tomato spotted wilt virus. Journal of General Virology 71:2801–2807
    [Google Scholar]
  7. de Ávila A. C., de Haan P., Kitajima E. W., Kormelink R., Resende R. de O., Goldbach R. W., Peters D. 1992a; Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from Impatiens sp. in the Netherlands. Journal of Phytopathology 134:133–151
    [Google Scholar]
  8. de Ávila A. C., de Haan P., Smeets M. L. L., Rjesende R. de O., Kitajima E. W., Goldbach R. W., Peters D. 1992b; Distinct levels of relationships between tospovirus isolates. Archives of Virology (in press)
    [Google Scholar]
  9. de Haan P. 1991 Exploring and exploiting the RNA genome of tomato spotted wilt virus Ph.D. thesis wageningen Agricultural University;
    [Google Scholar]
  10. de Haan P., Wagemakers L., Peters D., Goldbach R. 1990; The S RNA segment of tomato spotted wilt virus has an ambisense character. Journal of General Virology 71:1001–1007
    [Google Scholar]
  11. de Haan P., Kormelink R., Resende R. de O., van Poelwijk F., Peters D., Goldbach R. 1991; Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. Journal of General Virology 11:2207–2216
    [Google Scholar]
  12. de Haan P., de Ávila A. C., Kormelink R., Westerbroek A., Gielen J. J. L., Peters D., Goldbach R. 1992; The nucleotide sequence of the S RNA of Impatiens necrotic spot virus, a novel tospovirus. FEBS Letters 306:27–32
    [Google Scholar]
  13. Devereux J., Haeberli P., Smithes O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  14. Elliott R. M. 1990; Molecular biology ofthe Bunyaviridae. Journal of General Virology 71:501–522
    [Google Scholar]
  15. Francki R. I. B., Fauquet C. M., Knudson D. D., Brown F. 1991; Fifth report of the International Committeeon Taxonomy of Viruses. Archives of VirologySupplementum 21–450
    [Google Scholar]
  16. Giordi C., Accardi L., Nicoletti L., Takehara M. C. G., Hilditch C., Morikawa S., Bishop D. H. L. 1991; Sequences and coding strategies of the S RNAs of the Toscana and Rift Valley fever viruses compared to those of Punta Toro, Sicilian sandfly fever, and Uukuniemi viruses. Virology 180:738–753
    [Google Scholar]
  17. Gonzales-Scarano F., Nathanson N. 1990; Bunyaviruses. In Virology, 2nd edn. pp 1195–1228 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  18. Gubler U., Holman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  19. Kameya-Iwaki M., Hanada K., Honda Y., Tochira H. 1988; A watermelon strain of tomato spotted wilt virus (TSWV-W) and some properties of its nucleic acid.
    [Google Scholar]
  20. Kormelink R., Kitajima E. W., de Haan P., Zuidema D., Peters D., Goldbach R. 1991; The non-structural protein (NSs) encoded by the ambisense S RNA segment of tomato spotted wilt virus is associated with fibrous structures in infected plant cells. Virology 181:459–468
    [Google Scholar]
  21. Kormelink R., de Haan P., Peters D., Goldbach R. 1992a; Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants. Journal of General Virology 73:687–693
    [Google Scholar]
  22. Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R. 1992b; The nucleotide sequence ofthe M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. Journal of General Virology 73:2795–2804
    [Google Scholar]
  23. Law M. D., Moyer J. W. 1990; A tomato spotted wilt-like virus with a serologically distinct N protein. Journal of General Virology 71:933–938
    [Google Scholar]
  24. Law M. D., Speck J., Moyer J. W. 1991; Nucleotide sequence of the 3′ non-coding region and N gene of the S RNA of a serologicaliy distinct tospovirus. Journal of General Virology 72:2597–2601
    [Google Scholar]
  25. Law M. D., Speck J., Moyer J.W. 1992; The M RNA of Impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology 188:732–741
    [Google Scholar]
  26. Maiss E., Ivanova L., Breyel E., Adam G. 1991; Cloning and sequencing ofthe S RNA from a Bulgarian isolate of tomato spotted wilt virus. Journal of General Virology 72:461–464
    [Google Scholar]
  27. Marchoux G. 1990; La transmission de virus par Frankliniella occidentalis et autres thrips. Phytoma 422:40–45
    [Google Scholar]
  28. Peters D. 1991; Divergent evolution of Rhabdoviridae and Bunya-viridae in plants and animals. Seminars in Virology 2:27–37
    [Google Scholar]
  29. Peters D., de Ávila, A C., Kitajima E. W., Resende R. de O., de Haan P., Goldbach R. W. 1991; An overview of tomato spotted wilt virus. In Virus-Thrips-Plant Interactions of TSWV, Proceedings of the USDA Workshop, Beltsville, U.S.A. pp 1–14 Edited by Hsu H.-T., Lawson R. H. Springfield: National Technical Information Service;
    [Google Scholar]
  30. Reddy D. V. R., Sudarashana M. R., Ratna A. S., Amin P. W., Kumar I. K., Murphy A. K. 1991; The occurrence of yellow spot virus, a member of tomato spotted wilt virus group, on peanut (Arachis hypogea L.) in India. In Virus-Thrips-Plant Interactions of TSW, Proceedings of the USDA Workshop, Beltsville U.S.A. pp 77–78 Edited by Hsu H.-T., Lawson R. H. Springfield: National Technical Information Service;
    [Google Scholar]
  31. Reddy D. V. R., Ratna A. S., Sudarshana M. R., Poul F., Kumar I. K. 1992; Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Annals of Applied Biology 120:279–286
    [Google Scholar]
  32. Sakimura K. 1962; The present status of thrips-borne viruses. In Biological Transmission of Disease Agents pp 33–40 Edited by Maramorosch K. New York: Academic Press;
    [Google Scholar]
  33. Simons F. S., Hellman U. L. F., Pettersson R. F. 1990; Uukuniemi vims S RNA segment: ambisense coding strategy, packaging of complementary strands into virions and homology to members of the genus Phlebovirus. Journal of Virology 64:247–255
    [Google Scholar]
  34. Stohwasser R., Giebel L. B., Zoller L., Bautz E. K.F., Darai G. 1990; Molecular characterization of the RNA s segment of nephropathia epidemica virus strain Hallnas Bl. Virology 174:79–86
    [Google Scholar]
  35. Vaira A. M., Lisa V., Luisoni E. 1992; Diffusione di due ceppi di tomato spotted wilt virus in Ljnguria. Informatore Fitopatologico 2:59–63
    [Google Scholar]
  36. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  37. Zhang H., Scholl R., Browsc J., Somerville C. 1988; Double stranded DNA sequencing as a choice of DNA sequencing. Nucleic Acids Research 16:1220
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-2-153
Loading
/content/journal/jgv/10.1099/0022-1317-74-2-153
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error