1887

Abstract

The role of carbohydrate moieties on the immunoprotective ability of parainfluenza virus type 3 (PIV-3) haemagglutinin—neuraminidase (HN) and fusion (F) glycoproteins was tested in hamsters. HN and F proteins were purified from detergent-solubilized virus by lentillectin affinity chromatography and deglycosylated by treatment with endoglycosidase F (endo F). Immunization of hamsters with either 1 or 5 µg of mocktreated (glycosylated) affinity-purified proteins elicited strong haemagglutination inhibition and neutralizing antibody responses 4 weeks after the primary injection. In contrast, titres were significantly lower with endo F-treated (deglycosylated) proteins. However, following the booster doses with at least 5 µg of antigen, glycosylated and deglycosylated proteins induced comparable antibody titres. There was no significant difference in the ability of the glycosylated or deglycosylated proteins to protect either the upper or lower respiratory tracts of immunized hamsters against PIV-3 challenge. These results suggest that the carbohydrate moieties of the HN and F proteins are not necessary for eliciting a protective response in hamsters.

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1993-12-01
2024-04-25
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References

  1. Ambrose M. W., Wyde P. R., Ewasyshyn M., Bonneau A.-M., Caplan B., Meyer H. L., Klein M. 1991; Evaluation of the immunogenicity and protective efficacy of a candidate parainfluenza virus type 3 subunit vaccine in cotton rats. Vaccine 9:505–511
    [Google Scholar]
  2. Benjouad A., Gluckman J.-C., Rochat H., Montagnier L., Bahraoui E. 1992; Influence of carbohydrate moieties on the immunogenicity of human immunodeficiency virus type 1 recombinant gpl60. Journal of Virology 66:2473–2483
    [Google Scholar]
  3. Chanock R. M. 1975; Parainfluenza viruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections pp 611–632 Edited by Lennette E. H., Schmidt N. J. Washington, D.C.: American Public Health Association;
    [Google Scholar]
  4. Chanock R. M., McIntosh K. 1990; Parainfluenza virus. In Virology 2nd edn pp 963–988 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  5. Dorner A. J., Kaufman R. J. 1990; Analysis of synthesis, processing and secretion of proteins expressed in mammalian cells. Methods in Enzymology 185:577–596
    [Google Scholar]
  6. Elder J. H., Alexander S. 1982; Endo-β-N-acetyl- glucosaminidase F: endoglycosidase from Flavobacterium meningo-septicum that cleaves both high mannose and complex glycoproteins. Proceedings of the National Academy of Sciences, U.S.A. 79:4540–4544
    [Google Scholar]
  7. Elder J. H., McGee J. S., Alexander S. 1986; Carbohydrate side chains of Rauscher leukemia virus envelope glycoproteins are not required to elicit a neutralizing antibody response. Journal of Virology 57:340–342
    [Google Scholar]
  8. Emr S. D. 1990; Heterologous gene expression in yeast. Methods in Enzymology 185:231–280
    [Google Scholar]
  9. Ewasyshyn M., Caplan B., Bonneau A.-M., Scollard N., Graham S., Usman S., Klein M. 1992; Comparative analysis of the immunostimulatory properties of different adjuvants on theimmunogenicity of a prototype parainfluenza virus type 3 subunit vaccine. Vaccine 10:412–120
    [Google Scholar]
  10. Galinski M. S., Mink M. A., Pons M. W. 1987; Molecular cloning and sequence analysis of the human parainfluenza virus genes encoding the surface glycoproteins, F and HN. Virus Research 8:205–215
    [Google Scholar]
  11. Gerard C. 1990; Purification of glycoproteins. Methods in Enzymology 182:529–539
    [Google Scholar]
  12. Gorman W. L., Pridgen C., Portner A. 1991; Glycosylation of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 1 affects its functional but not its antigenic properties. Virology 183:83–90
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lambert D. R. 1988; Role of oligosaccharides in the structure and function of respiratory syncytial virus glycoproteins. Virology 164:458–466
    [Google Scholar]
  15. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Bio/Technology 6:47–55
    [Google Scholar]
  16. Morrison T. G. 1988; Structure, function, and intracellular processing of paramyxovirus membrane proteins. Virus Research 10:113–136
    [Google Scholar]
  17. Murphy B. R., Prince G. A., Collins P. L., Coelingh K., Olmsted R. A., Spriggs M. K., Parrott R. H., Kim H.-W., Brandt C. D., Chanock R. 1989; Current approaches to the development of vaccines effective against parainfluenza and respiratory syncytial virus. Virus Research 11:1–15
    [Google Scholar]
  18. Nakamura K., Homma M., Compans R. W. 1982; Effect of tunicamycin on the replication of Sendai virus. Virology 119:474–487
    [Google Scholar]
  19. Peterson G. A. 1977; A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  20. Ray R., Glaze B. J., Compans R. W. 1988; Role of individual glycoproteins of human parainfluenza virus type 3 in the induction of a protective immune response. Journal of Virology 62:783–787
    [Google Scholar]
  21. Ray R., Galinski M. S., Compans R. W. 1989; Expression of the fusion glycoprotein of human parainfluenza type 3 virus in insect cells by a recombinant baculovirus and analysis of its immunogenic property. Virus Research 12:169–180
    [Google Scholar]
  22. Sjöblom I., Lundström M., Sjögren-Jansson E., Glorioso J. C., Jeansson S., Olofsson S. 1987; Demonstration and mapping of highly carbohydrate-dependent epitopes in the herpes simplex virus type 1-specified glycoprotein C. Journal of General Virology 68:545–554
    [Google Scholar]
  23. Spriggs M. K., Murphy B. R., Prince G. A., Olmsted R. A., Collins P. L. 1987; Expression of the F and HN glycoproteins of human parainfluenza type 3 by recombinant vaccinia viruses: contributions of the individual proteins to host immunity. Journal of Virology 61:3416–3423
    [Google Scholar]
  24. Stanley P., Narasimhan S., Siminovitch L., Schachter H. 1975; Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine glycoprotein N-acetylglucosaminyltransferase activity. Proceedings of the National Academy of Sciences, U.S.A. 72:3323–3327
    [Google Scholar]
  25. Takatsuki A., Tamura G. 1971; Tunicamycin, a new antibiotic; some biological properties of the antiviral activity of tunicamycin. Journal of Antibiotics 24:224–231
    [Google Scholar]
  26. Van Drunen Littel-Van den Hurk S., Hughes G., Babiuk L. A. 1990; The role of carbohydrate in the antigenic and immunogenic structure of bovine herpesvirus type 1 glycoproteins gI and gIV. Journal of General Virology 71:2053–2063
    [Google Scholar]
  27. Van Wyke Coelingh K., Murphy B., Collins P. L., Legacq-Verheyden A.-M., Battey J. F. 1987; Expression of biologically active and antigenically authentic parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein by a recombinant baculovirus. Virology 160:465–172
    [Google Scholar]
  28. Vidal S., Mottet G., Kolakofsky D., Roux L. 1989; Addition of high mannose sugars must precede disulfide bond formation for proper folding of Sendai virus glycoproteins. Journal of Virology 63:892–900
    [Google Scholar]
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