Susceptibility of transgenic tobacco plants expressing tobacco rattle virus coat protein to nematode-transmitted and mechanically inoculated tobacco rattle virus
Transgenic Samsun NN tobacco plants expressing the coat protein of tobacco rattle virus were exposed to mechanical leaf inoculation with tobacco rattle virus and to viruliferous trichodorid vector nematodes. Whereas plants were resistant to mechanical inoculation the vector nematodes successfully transmitted tobacco rattle virus to the roots as well as to the leaves of these plants. It is suggested that transgenic resistance is overcome either because vector nematodes inject relatively large numbers of virus particles into a cell or because they inject destabilized particles. The results indicate that coat protein-mediated resistance is unlikely to be of value for controlling tobacco rattle virus in field crops.
AngenentG. C.,
LinthorstH. J. M.,
van BelkumA. F.,
CornelissenB. J. C.,
BolJ. F.1986; RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14:4673–4682
AngenentG. C.,
PosthumusE.,
BrederodeF. Th.,
BolJ. F.1989; Genome structure of tobacco rattle virus strain PLB, further evidence on the occurrence of RNA recombination among tobra-viruses. Virology 171:271–274
AngenentG. C.,
van den OuwelandJ. M. W.,
BolJ. F.1990; Susceptibility to virus infection of transgenic tobacco plants expressing structural and nonstructural genes of tobacco rattle virus. Virology 175:191–198
BrownD. J. F.,
PloegA. T.,
RobinsonD. J.1989; A review of reported associations between Trichodorus and Paratrichodorus species (Nematoda, Trichodoridae) and tobraviruses with a description of laboratory methods for examining virus transmission by trichodorids. Revue de Nématologie 12:235–241
CadmanC. H.,
HarrisonB. D.1959; Studies on the properties of soil-borne viruses of the tobacco-rattle type occurring in Scotland. Annals of Applied Biology 47:542–556
CornelissenB. J. C.,
LinthorstH. J. M.,
BrederodeF. T.,
BolJ. F.1986; Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14:2157–2169
HarrisonB. D.,
RobinsonD. J.1986; Tobraviruses. In The Plant Viruses vol 2 pp 339–369 Edited by
van RegenmortelV. M. H.Fraenkel-ConratH.
New York & London: Plenum Press;
HemenwayC.,
FangR. X.,
KaniewskiW. K.,
ChuaN. H.,
TumerN. E.1988; Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO Journal 7:1273–1280
LangeveldS. A.,
DoreJ. M.,
MemelinkJ.,
DerksA. F. L. M.,
van der VlugtC. I. M.,
AsjesC. J.,
BolJ. F.1991; Identification of potyviruses using the polymerase chain reaction with degenerate primers. Journal of General Virology 72:1531–1541
LawsonC.,
KaniewskiW.,
HaleyL.,
RozmannR.,
NewellC.,
SandersP.,
TumerN. E.1990; Engineering resistance to mixed virus infection in a commercial potato cultivar, resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology 8:127–134
MarshJ. L.,
ErfleM.,
WykesE. J.1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485
PloegA. T.,
BrownD. J. F.,
RobinsonD. J.1992a; Acquisition and subsequent transmission of tobacco rattle virus isolates by Paratrichodorus and Trichodorus nematode species. Netherlands Journal of Plant Pathology 98:291–300
PloegA. T.,
BrownD. J. F.,
RobinsonD. J.1992b; The association between species of Trichodorus and Paratrichodorus vector nematodes and serotypes of tobacco rattle tobravirus. Annals of Applied Biology 121:619–630
Powell AbelP.,
NelsonR. S.,
DeB.,
HoffmannN.,
RogersS. G.,
FraleyR. T.,
BeachyR. N.1986; Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743
QuemadaH. D.,
GonsalvesD.,
SlightomJ. L.1991; Expression of coat protein gene from cucumber mosaic virus strain C in tobacco: protection against infections by CMV strains transmitted mechanically or by aphids. Phytopathology 81:794–802
RegisterJ. C.,
BeachyR. N.1988; Resistance to TMV in transgenic plants results from interference with an early event in infection. Virology 166:524–532
StarkD. M.,
BeachyR. N.1989; Protection against potyvirus infection in transgenic plants, evidence for broad-spectrum resistance. Bio/Technology 7:1257–1262
TumerN. E.,
O’ConnelK. M.,
NelsonS.,
SandersP. R.,
BeachyR. N.,
FraleyR. T.,
ShahD.1987; Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO Journal 6:1181–1188
Van Der WilkF.,
Posthumus-Lutke WillinkD.,
FIuismanM. J.,
FIuttingaH.,
GoldbachR.1991; Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. Plant Molecular Biology 17:431–439
Van DunC. P. M.,
BolJ. F.1988; Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167:649–652
Van DunC. M. P.,
BolJ. F.,
van Vloten DotingL.1987; Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159:299–305
Van Vloten-DotingL.,
JasparsE. M. J.1967; Enhancement of infectivity by combination of two ribonucleic acid components from alfalfa mosaic virus. Virology 33:684–693
WyssU.1975; Feeding of Trichodorus, Longidorus and Xiphinema. In Nematode Vectors of Plant Viruses pp 203–221 Edited by
LambertiF.,
TaylorC. E.,
SeinhorstJ. W.
New York & London: Academic Press;
Susceptibility of transgenic tobacco plants expressing tobacco rattle virus coat protein to nematode-transmitted and mechanically inoculated tobacco rattle virus