1887

Abstract

PCR was used to amplify and sequence the complete HA1 region of the haemagglutinin (HA)-encoding genes of 10 clinical isolates of influenza virus of the H1N1 or H3N2 subtypes. These sequences were compared to those obtained from viruses isolated from the same specimens after passage in eggs and MDCK cells. Amino acid substitutions in the egg-derived HA sequences were found in nine out of the 10 specimens analysed, whereas seven out of eight of the MDCK-derived HA sequences were identical to those in the corresponding original specimens. Changes in the H1 HA occurred at residues 77a, 196 (also found in the corresponding HA from the MDCK isolate), 225, 226 and 227; changes in the H3 HA occurred at residues 137, 156, 186, 248 and 276. In addition, we have shown that an amino acid change at residue 145 in the HA of the H3 subtype that was previously demonstrated to be egg-selected is now present in circulating strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-11-2513
1993-11-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/11/JV0740112513.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-11-2513&mimeType=html&fmt=ahah

References

  1. Both G. W., Sleigh M. J., Cox N. J., Kendal A. P. 1983; Antigenic drift in influenza virus H3 hemagglutinin from 1968 to 1980: multiple evolutionary pathways and sequential amino acid changes at key antigenic sites. Journal of Virology 48:52–60
    [Google Scholar]
  2. Caton A., Brownlee G. G., Yewdell W. J., Gerhard W. 1982; The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell 31:417–427
    [Google Scholar]
  3. Domingo E. 1989; RNA virus evolution and the control of viral disease. In Progress in Drug Research vol 33 pp 93–133 Edited by Jucker E. Basel: Birkhauser Verlag;
    [Google Scholar]
  4. Domingo E., Holland J. J. 1988; High error rates, population equilibrium and evolution of RNA replication systems. In RNA Genetics vol 3 pp 3–36 Edited by Domingo E., Ahlquist P., Holland J. J. Boca Raton: CRC Press;
    [Google Scholar]
  5. Katz J. M., Webster R. G. 1988; Antigenic and structural characterization of multiple subpopulations of H3N2 influenza virus from an individual. Virology 165:446–456
    [Google Scholar]
  6. Katz J. M., Naeve C. W., Webster R. G. 1987; Host cell-mediated variation in H3N2 influenza viruses. Virology 156:386–395
    [Google Scholar]
  7. Katz J. M., Wang M., Webster R. G. 1990; Direct sequencing of the HA gene of influenza (H3N2) virus in original clinical samples reveals sequence identity with mammalian cell-grown virus. Journal of Virology 64:1808–1811
    [Google Scholar]
  8. Klimov A. I., Cox N. J., Yotov W. V., Rocha E., Alexandrova G. I., Kendal A. P. 1992; Sequence changes in the live attenuated, cold-adapted variants of influenza A/Leningrad/134/57 (H2N2) virus. Virology 186:795–797
    [Google Scholar]
  9. Mullis K. B., Falona F. A. 1987; Specific synthesis of DNA in vitro via a polymerase-catalysed chain reaction. Methods in Enzymology 155:335–350
    [Google Scholar]
  10. Rajakumar A., Swerkosz E. M., Schulze I. T. 1990; Sequence of an influenza virus hemagglutinin determined directly from a clinical sample. Proceedings of the National Academy of Sciences, U.S.A 87:4154–4158
    [Google Scholar]
  11. Robertson J. S., Naeve C. W., Webster R. G., Bootman J. S., Newman R., Schild G. C. 1985; Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs. Virology 143:166–174
    [Google Scholar]
  12. Robertson J. S., Bootman J. S., Newman R., Oxford J. C., Daniels R. S., Webster R. G., Schild G. C. 1987; Structural changes in the hemagglutinin which accompany egg adaptation of an influenza A (H1N1) virus. Virology 160:31–37
    [Google Scholar]
  13. Robertson J. S., Nicolson C., Bootman J. S., Major D., Robertson E. W., Wood J. M. 1991; Sequence analysis of the haemagglutinin (HA) of influenza A (H1N1) viruses present in clinical material and comparison with the HA of laboratory-derived virus. Journal of General Virology 72:2671–2677
    [Google Scholar]
  14. Rocha E., Cox N. J., Black R. A., Harmon M. W., Harrison C. J., Kendal A. P. 1991; Antigenic and genetic variation in influenza A (H1N1) virus isolates recovered from a persistently infected immunodeficient child. Journal of Virology 65:2340–2350
    [Google Scholar]
  15. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  16. Schild G. C., Oxford J. S., De Jong J. C., Webster R. G. 1983; Evidence for host-cell selection of influenza virus antigenic variants. Nature, London 303:706–709
    [Google Scholar]
  17. Wang M., Katz J. M., Webster R. G. 1989; Extensive heterogeneity in the hemagglutinin of egg-grown influenza viruses from different patients. Virology 171:275–279
    [Google Scholar]
  18. Weiss W., Brown J. H., Cusack S., Paulson J. C., Skehel J. J., Wiley D. C. 1988; Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid. Nature, London 333:426–431
    [Google Scholar]
  19. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature, London 289:373–378
    [Google Scholar]
  20. Wilson I. A., Skehel J. J., Wiley D. C. 1981; Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature, London 289:366–373
    [Google Scholar]
  21. Winter G., Fields S., Brownlee G. G. 1981; Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype. Nature, London 292:72–75
    [Google Scholar]
  22. Xu X., Rocha E., Regnery H., Kendal A. P., Cox N. J. 1993; Genetic and antigenic analyses of influenza A (H1N1) virus, 1986–1991. Virus Research 28:37–55
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-11-2513
Loading
/content/journal/jgv/10.1099/0022-1317-74-11-2513
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error