Reasons for the low accumulation level of aphid transmission factor protein in infected leaves with an aphid-non-transmissible cauliflower mosaic virus isolate, CM1841 Free

Abstract

The synthesis and accumulation of aphid transmission factor protein (p18) in cauliflower mosaic virus (CaMV)-infected turnip protoplasts were examined in time course and pulse-labelling experiments, comparing an aphid-non-transmissible isolate (CM1841) with an recombinant aphid-transmissible CaMV (CMBX) generated from the CM1841 isolate. There was little difference in the synthesis and accumulation of p18 between CM1841- and CMBX-infected protoplasts. When the accumulation of p18 in infected leaves was monitored from 3 to 28 days post-symptom emergence (p.e.) by Western blotting, the amount of p18 accumulated in CM1841-infected leaves continuously decreased from 3 days p.e. throughout the experimental period, whereas the amount of p18 in CMBX-infected leaves was lowest at 3 days p.e. and increased thereafter. These results suggested that CM1841 differed from CMBX not in the synthesis of p18 but in the stability of p18 in infected leaves.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-11-2469
1993-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/11/JV0740112469.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-11-2469&mimeType=html&fmt=ahah

References

  1. Armour S. L., Melcher U., Pirone T. P., Lyttle D. J., Essenberg R. C. 1983; Helper component for aphid transmission encoded by region II of cauliflower mosaic virus DNA. Virology 129:25–30
    [Google Scholar]
  2. Blanc S., Cerutti M., Usmany M., Vlak J. M., Hull R. 1993a; Biological activity of cauliflower mosaic virus aphid transmission factor expressed in a heterologous system. Virology 192:643–650
    [Google Scholar]
  3. Blanc S., Cerutti M., Chaabihi H., Louis C., Devauchelle G., Hull R. 1993b; Gene II product of aphid-nontransmissible isolate of cauliflower mosaic virus expressed in baculovirus system possesses aphid transmission factor activity. Virology 192:651–654
    [Google Scholar]
  4. Espinoza A. M., Medina V., Hull R., Markham P. G. 1991; Cauliflower mosaic virus gene II product forms distinct inclusion bodies in infected plant cells. Virology 185:337–344
    [Google Scholar]
  5. Furusawa I., Okuno T. 1978; Infection with BMV of mesophyll protoplasts isolated from five plant species. Journal of General Virology 40:489–491
    [Google Scholar]
  6. Furusawa I., Yamaoka N., Okuno T., Yamamoto M., Kohno M., Kunoh H. 1980; Infection of turnip protoplasts with cauliflower mosaic virus. Journal of General Virology 48:431–435
    [Google Scholar]
  7. Gardner R. C., Howarth A. J., Hahn P., Brown-Luedi M., Shepherd R. J., Messing J. 1981; The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing. Nucleic Acids Research 9:2871–2888
    [Google Scholar]
  8. Harker C. L., Woolston C. J., Markham P. G., Maule A. J. 1987; Cauliflower mosaic virus aphid transmission factor protein is expressed in cells infected with some aphid nontransmissible isolates. Virology 160:252–254
    [Google Scholar]
  9. Horikoshi M., Mise K., Furusawa I., Shishiyama J. 1988; Immunological analysis of brome mosaic virus replication. Journal of General Virology 69:3081–3087
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Lung M. C. Y., Pirone T. P. 1973; Studies on the reason for differential transmissibility of cauliflower mosaic virus isolates by aphids. Phytopathology 63:910–914
    [Google Scholar]
  12. Lung M. C. Y., Pirone T. P. 1974; Acquisition factor required for aphid transmission of purified cauliflower mosaic virus. Virology 60:260–264
    [Google Scholar]
  13. Maule A. J., Hull R., Donson J. 1985; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6:215–224
    [Google Scholar]
  14. Nakayashiki H., Kobayashi K., Tsuge S., Okuno T., Furusawa I. 1991; Characterization of in vitro recombinant cauliflower mosaic virus in aphid transmissibility. Annals of the Phytopathological Society of Japan 57:634–640
    [Google Scholar]
  15. Nilsson B., Abrahmsen L., Uhlen M. 1985; Immobilization and purification of enzymes with staphylococcal Protein A gene fusion vectors. EMBO Journal 4:1075–1080
    [Google Scholar]
  16. Tsuge S., Kobayashi K., Nakayashiki H., Okuno T., Furusawa I. 1993; Replication of cauliflower mosaic virus ORF I mutants in turnip protoplasts. Annals of the Phytopathological Society of Japan (in press)
    [Google Scholar]
  17. Woolston C. J., Covey S. N., Penswick J. R., Davies J. W. 1983; Aphid transmission and a polypeptide are specified by a defined region of the cauliflower mosaic virus genome. Gene 23:15–23
    [Google Scholar]
  18. Woolston C. J., Czaplewski L. G., Markham P. G., Goad A. S., Hull R., Davies J. W. 1987; Location and sequence of a region of cauliflower mosaic virus gene 2 responsible for aphid transmissibility. Virology 160:246–251
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-11-2469
Loading
/content/journal/jgv/10.1099/0022-1317-74-11-2469
Loading

Data & Media loading...

Most cited Most Cited RSS feed