1887

Abstract

Isolates of citrus exocortis viroid (CEV) from a single sweet orange citrus source have been selected by sequential passage through the alternative hosts citron, , a hybrid tomato , and from disorganized callus culture of the hybrid tomato. The distinctions in symptom expression, titre and electrophoretic mobility among the CEV isolates, operationally termed CEVc (citron), CEVg (), CEVt (tomato) and CEVcls (callus) are supported by characteristically different nucleotide sequences. The nucleotide sequence of full-length cDNA clones of CEVc purified from citron shows exchanges not reported for any previously described CEV variant. An unusual number of exchanges have been localized in the terminal domains of all the isolates analysed here. A common pattern of nucleotide exchanges, described as a ‘tomato signature’, can be detected in all of the isolates derived from hybrid tomato tissues.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-11-2427
1993-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/11/JV0740112427.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-11-2427&mimeType=html&fmt=ahah

References

  1. Benton R. J., Bowman F. T., Fraser L., Kebby R. G. 1950; Stunting and scaly butt of citrus associated with Poncirus trifoliala rootstock. Agricultural Gazette of New South Wales 61:20–22
    [Google Scholar]
  2. Donis-Keller H., Browning K. S., Clark J. M. 1981; Sequence heterogeneity in satellite tobacco necrosis virus RNA. Virology 110:43–54
    [Google Scholar]
  3. Duran-Vila N., Semancik J. S. 1982; Effects of exogenous auxins on tomato tissue infected with the citrus exocortis viroid. Phytopathology 72:777–781
    [Google Scholar]
  4. Garcia-Arenal F., Pallas V., Flores R. 1987; The sequence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid. Nucleic Acids Research 15:4203–1210
    [Google Scholar]
  5. Gross H. J., Krupp G., Domdey H., Raba M., Alberty H., Lossow C. H., Ramm K., Sanger H. L. 1982; Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid. European Journal of Biochemistry 121:249–257
    [Google Scholar]
  6. Igloi G. L. 1983; A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Analytical Biochemistry 134:184–188
    [Google Scholar]
  7. Keese P., Symons R. H. 1985; Domains in viroids: evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proceedings of the National Academy of Sciences, U.S.A. 82:4582–4586
    [Google Scholar]
  8. Kurath G., Palukaitis P. 1990; Serial passage of infectious transcripts of a cucumber mosaic virus satellite RNA clone results in sequence heterogeneity. Virology 176:8–15
    [Google Scholar]
  9. Lin J. J., Semancik J. S. 1985; Coordination between host nucleic acid metabolism and citrus exocortis viroid turnover. Virus Research 3:213–230
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook T. 1982 Molecular Cloning: A Laboratory Manual pp 129–132–230ߝ234 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Marton L., Duran-Vila N., Lin J. J., Semancik J. S. 1982; Properties of cell cultures containing the citrus exocortis viroid. Virology 122:229–238
    [Google Scholar]
  12. Mishra M. D., Hammond R. W., Owens R. A., Smith D. R., Diener T. O. 1991; Indian bunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid. Journal of General Virology 72:1781–1785
    [Google Scholar]
  13. Rivera-Bustamante R., Gin R., Semancik J. S. 1986; Enhanced resolution of circular and linear molecular forms of viroid and viroid-like RNA by electrophoresis in a discontinuous-pH system. Analytical Biochemistry 15:91–95
    [Google Scholar]
  14. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  15. Semancik J. S., Harper K. L. 1984; Optimal conditions for cell-free synthesis of citrus exocortis viroid and the question of specificity of RNA polymerase activity. Proceedings of the National Academy of Sciences, U.S.A 81:4429–1433
    [Google Scholar]
  16. Semancik J. S., Szychowski J. A. 1992; Relationships among the viroids derived from grapevines. Journal of General Virology 73:1465–1469
    [Google Scholar]
  17. Semancik J. S., Roistacher C. N., Rivera-Bustamante R., Duran-Vila N. 1988; Citrus cachexia viroid, a new viroid of citrus: relationship to viroids of the exocortis disease complex. Journal of General Virology 69:3059–3068
    [Google Scholar]
  18. Shikata E. 1990; New viroids from Japan. Seminars in Virology 1:107–115
    [Google Scholar]
  19. Szychowski J. A., Goheen A. C., Semancik J. S. 1988; Mechanical transmission and rootstock reservoirs as factors in the widespread distribution of viroids in grapevines. American Journal of Enology and Viticulture 39:213–216
    [Google Scholar]
  20. Visvader J. E., Symons R. H. 1983; Comparative sequence and structure of different isolates of citrus exocortis viroid. Virology 130:232–237
    [Google Scholar]
  21. Visvader J. E., Symons R. H. 1985; Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity. Nucleic Acids Research 13:2907–2920
    [Google Scholar]
  22. Visvader J. E., Symons R. H. 1986; Replication of in vitro constructed viroid mutants: location of the pathogenicity-modulating domain of citrus exocortis viroid. EMBO Journal 5:2051–2055
    [Google Scholar]
  23. Visvader J. E., Gould A. R., Bruening G. E., Symonds R. H. 1982; Citrus exocortis viroid: nucleotide sequence and secondary structure of an Australian isolate. FEBS Letters 137:288–292
    [Google Scholar]
  24. Weathers L. G., Greer F. C. Jr, Harjung M. K. 1967; Transmission of exocortis virus of citrus to herbaceous plants. Plant Disease Reporter 51:868–871
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-11-2427
Loading
/content/journal/jgv/10.1099/0022-1317-74-11-2427
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error