1887

Abstract

We have studied the induction of the cytotoxic T lymphocyte (CTL) response to measles virus (MV) antigens expressed as vaccinia virus (VV) recombinants in a murine model. In C3H mice (H-2) only the nucleoprotein (NP) induced a CTL response and this was shown to be cross-reactive with the closely related canine distemper virus (CDV). The presentation of this antigen was shown to be K-restricted. In BALB/c mice (H-2), both the haemagglutinin (HA) and the NP induced a strong CTL response, the former being serotype-specific, whereas the latter cross-reacted with CDV. Both responses were found to be L-restricted. Based on the prediction for L T cell motifs, we tested a number of MV NP-derived nonapeptides for their capacity to sensitize P815 cells (H-2) for lysis by spleen cells from VV-NP-immunized mice. One of these peptides, comprising amino acids 281 to 289 (Tyr-Pro-Ala-Leu-Gly-Leu-His-Glu-Phe) was as effective as cells expressing the complete NP protein. This motif is conserved in the CDV NP.

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/content/journal/jgv/10.1099/0022-1317-74-11-2357
1993-11-01
2019-11-18
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-11-2357
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