1887

Abstract

The goal of experiments reported here was to identify the genes that encode capsid proteins VP21 and VP24 of herpes simplex virus type 1 (HSV-1). Capsids were isolated from infected cells and the proteins were separated by SDS-PAGE. N-terminal amino acid sequence analysis of partial CNBr digestion products, and of intact VP21, showed that it is encoded within the UL26 open reading frame (ORF) of HSV-1 beginning with codon 248 and probably extending to the end of the ORF (codon 635). Similar analysis of digestion products confirmed that VP24 is specified by codons 1 to 247 at the 5′ end of the UL26 ORF. Each of the seven known capsid proteins has now been assigned to an ORF.

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1993-10-01
2024-10-10
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References

  1. Baker T. S., Newcomb W. W., Booy F. P., Brown J. C., Steven A. C. 1990; Three-dimensional structures of maturable and abortive capsids of equine herpes virus 1 from cryoelectron microscopy. Journal of Virology 64:563–573
    [Google Scholar]
  2. Braun D. K., Roizman B., Pereira L. 1984; Characterization of post-translational products of herpes simplex virus gene 35 proteins binding to the surfaces of full capsids but not empty capsids. Journal of Virology 49:142–153
    [Google Scholar]
  3. Cohen G. H., Ponce de Leon M., Diggelmann H., Lawrence W. C., Vernon S. K., Eisenberg R. J. 1980; Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2. Journal of Virology 34:521–531
    [Google Scholar]
  4. Costa R. H., Cohen G., Eisenberg R., Long D., Wagner E. 1984; Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0-23 and 0-27 map units encodes the major capsid protein VP5. Journal of Virology 49:287–292
    [Google Scholar]
  5. Davison A. J., Scott J. E. 1986; DNA sequence of the major capsid protein gene of herpes simplex virus type 1. Journal of General Virology 67:2279–2286
    [Google Scholar]
  6. Davison M. D., Rixon F. J., Davison A. J. 1992; Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1. Journal of General Virology 73:2709–2713
    [Google Scholar]
  7. Dbckman I. C., Hagen M., McCann P. J. 1992; Herpes simplex virus type 1 protease expressed in Escherichia coli exhibits autoprocessing and specific cleavage of the ICP35 assembly protein. Journal of Virology 66:7362–7367
    [Google Scholar]
  8. Desai P., DeLuca N. A., Glorioso J. C., Person S. 1993; Mutations in herpes simplex virus type 1 genes encoding VP5 and VP23 abrogate capsid formation and cleavage of replicated DNA. Journal of Virology 67:1357–1364
    [Google Scholar]
  9. Dilanni C. L., Drier D. A., Deckman I. C., McCann P. J., Liu F., Roizman B., Colonno R. J., Cordingley M. G. 1993; Identification of the herpes simplex virus-1 protease cleavage sites by direct sequence analysis of autoproteolytic cleavage products. Journal of Biological Chemistry 268:2048–2051
    [Google Scholar]
  10. Gibson W., Roizman B. 1972; Proteins specified by herpes simplex virus: VIII. Characterization and composition of multiple capsid forms of subtypes 1 and 2. Journal of Virology 10:1044–1052
    [Google Scholar]
  11. Gibson W., Marcy A. I., Comolli J. C., Lee J. 1990; Identification of precursor to cytomegalovirus capsid assembly protein and evidence that processing results in loss of its carboxy-terminal end. Journal of Virology 64:1241–1249
    [Google Scholar]
  12. Heilman C. J., Zweig M., Stephenson J. R., Hampar B. 1979; Isolation of nucleocapsid proteins of herpes simplex virus types 1 and 2 possessing immunologically type specific and cross-reactive determinants. Journal of Virology 29:34–42
    [Google Scholar]
  13. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus: XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  14. Holland L. E., Sandri-Goldin R. M., Goldin A. L., Glorioso J. C., Levine M. 1984; Transcriptional and genetic analyses of the herpes simplex virus type 1 genome: coordinates 0.29 to 0.45. Journal of Virology 49:947–959
    [Google Scholar]
  15. Liu F., Roizman B. 1991a; The promoter, transcriptional unit, and coding sequence of herpes simplex family 35 proteins are contained within and in frame with the UL26 open reading frame. Journal of Virology 65:206–212
    [Google Scholar]
  16. Liu F., Roizman B. 1991b; The herpes simplex virus type 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate. Journal of Virology 65:5149–5156
    [Google Scholar]
  17. Liu F., Roizman B. 1993; Characterization of the protease and other products of amino-terminus-proximal cleavage of the herpes simplex virus 1 UL26 protein. Journal of Virology 67:1300–1309
    [Google Scholar]
  18. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNae D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  19. McNab D. S., Courtney R. J. 1992; Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame. Journal of Virology 66:2653–2663
    [Google Scholar]
  20. Matsudaira P. 1987; Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry 262:10035–10038
    [Google Scholar]
  21. Newcomb W. W., Brown J. C. 1989; Use of Ar+ plasma etching to localize structural proteins in the capsid of herpes simplex virus type 1. Journal of Virology 63:4697–4702
    [Google Scholar]
  22. Newcomb W. W., Brown J. C. 1991; Structure of the herpes simplex virus capsid: effects of extraction with guanidine hydrochloride and partial reconstitution of extracted capsids. Journal of Virology 65:613–620
    [Google Scholar]
  23. Pertuiset B., Boccara M., Cebrian J., Berthelot N., Chou-sterman S., Puvion-Dutilleul F., Sisman J., Sheldrick P. 1989; Physical mapping and nucleotide sequence of a herpes simplex virus type 1 gene required for capsid assembly. Journal of Virology 63:2169–2179
    [Google Scholar]
  24. Preston V. G., Coates J. A. V., Rixon F. J. 1983; Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA. Journal of Virology 45:1056–1064
    [Google Scholar]
  25. Preston V. G., Rixon F. J., McDougall I. M., McGregor M., Al Kobaisi M. F. 1992; Processing of the herpes simplex viral assembly protein ICP35 near its C-terminal end requires the product of the whole of the UL26 reading frame. Virology 186:87–98
    [Google Scholar]
  26. Rixon F. J., Cross A. M., Addison C., Preston V. G. 1988; The products of herpes simplex virus type 1 gene UL26 which are involved in DNA packaging are strongly associated with empty but not with full capsids. Journal of General Virology 69:2879–2891
    [Google Scholar]
  27. Rixon F. J., Davison M. D., Davison A. J. 1990; Identification of the genes encoding two capsid proteins of herpes simplex virus type 1 by direct amino acid sequencing. Journal of General Virology 71:1211–1214
    [Google Scholar]
  28. Schagger H., von Jagow G. 1987; Tricine-sodium dodecyI sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry 166:368–379
    [Google Scholar]
  29. Schenk P., Woods A. S., Gibson W. 1991; The 45-kilodalton protein of cytomegalovirus (Coburn) B-capsids is an amino terminal extension form of the assembly protein. Journal of Virology 65:1525–1529
    [Google Scholar]
  30. Telford E. A. R., Watson M. S., McBride K., Davison A. J. 1992; The DNA sequence of equine herpesvirus-1. Virology 189:304–316
    [Google Scholar]
  31. Welch A. R., Woods A. S., McNally L. M., Cotter R. J., Gibson W. 1991; A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain, and cleavage site. Proceedings of the National Academy of Sciences, U,. S,. A. 88:10792–10796
    [Google Scholar]
  32. Weller S. K., Carmichael E. P., Aschman D. P., Goldstein D. J., Schaffer P. A. 1987; Genetic and phenotypic characterization of mutants in four essential genes that map to the left half of the HSV-1 UL DNA. Virology 161:198–210
    [Google Scholar]
  33. Zweig M., Heilman C. J., Rabin H., Hampar B. 1980; Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. Journal of Virology 35644–652
    [Google Scholar]
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