1887

Abstract

The genomic RNA of the potato virus X (PVX) strain HB, isolated in Bolivia and able to overcome all known resistance genes, has been cloned and sequenced. The PVX RNA sequence is 6432 nucleotides long and contains, similarly to the RNAs of other PVX strains, five open reading frames encoding proteins of s 165.1K, 24.5K, 12.4K, 7.6K and 25.1K (coat protein), respectively. Multiple amino acid sequence alignments of the coat proteins of four PVX strains identified eight amino acid residues unique for PVX. Structural prediction comparisons of the coat proteins of PVX and of the other strains suggest a general structural similarity. However, two of the eight amino acid residues unique for strain HB gave rise to a change in the predicted coat protein structure, suggesting a possible involvement in the resistance-breaking activity of PVX.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-10-2251
1993-10-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/10/JV0740102251.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-10-2251&mimeType=html&fmt=ahah

References

  1. Bercks R. 1970; Potato virus X. CMI/AAB Descriptions of Plant Viruses. no. 4
    [Google Scholar]
  2. Chou P. Y., Fasman G. D. 1978; Empirical predictions of protein conformation. Annual Review of Biochemistry 47:251–276
    [Google Scholar]
  3. Cockerham G. 1955; Strains of potato virus X. Proceedings of the Second Conference on Potato Virus Diseases, Lisse-Wageningen, 1954 pp 89–92
    [Google Scholar]
  4. Cockerham G. 1970; Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348
    [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–397
    [Google Scholar]
  6. Fribourg C. E. 1975; Studies on potato virus X strains isolated from Peruvian potatoes. Potato Research 18:216–226
    [Google Scholar]
  7. Hammond J., Lawson R. H. 1988; An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue. Journal of Virological Methods 20:203–217
    [Google Scholar]
  8. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  9. Jones R. A. C. 1985; Further studies on resistance-breaking strains of potato virus X. Plant Pathology 34:182–189
    [Google Scholar]
  10. Kamer G., Argos P. 1984; Primary structural comparisons of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 9:7269–7282
    [Google Scholar]
  11. Kavanagh T., Goulden M., Santa Cruz S., Chapman S., Barker I., Baulcombe D. 1992; Molecular analysis of a resistancebreaking strain of potato virus X. Virology 189:609–617
    [Google Scholar]
  12. Moreira A., Jones R. A. C., Fribourg C. E. 1980; Properties of a resistance-breaking strain of potato virus X. Annals of Applied Biology 95:93–103
    [Google Scholar]
  13. Orman B. E., Celnik R. M., Mandel A. M., Torres H. N., Mentaberry A. N. 1990; Complete cDNA sequence of a South American isolate of potato virus X. Virus Research 16:293–306
    [Google Scholar]
  14. Querci M., Salazar L. F., Fernandez-Northcote E. N. 1993; Detection of Andean potato virus X isolates by radioactive and nonradioactive nucleic acid spot hybridization tests. Phytopathology 83:171–176
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  17. Santa-Cruz S., Baulcombe D. C., Kavanagh T. 1989; Virulence and virulence determinants in potato virus X. Annual Report IPSR/JII/SL pp 43–44
    [Google Scholar]
  18. Skryabin K. G., Kraev A. S., Morozov S. Yu., Rozanov M. N., Chernov B. K., Lukasheva L. I., Atabekov J. G. 1988; The nucleotide sequence of potato virus X RNA. Nucleic Acids Research 16:10929–10930
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-10-2251
Loading
/content/journal/jgv/10.1099/0022-1317-74-10-2251
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error