1887

Abstract

The nucleotide sequences of the coat protein genes and 3′ non-translated regions (3′-NTRs) of three isolates of bean common mosaic virus (NL1, NL3 and NY15) and one isolate of blackeye cowpea mosaic virus (W) were determined. Comparison of these sequences revealed that the coat proteins of NL1, NY15 and W were identical in size (287 amino acids) and exhibited an overall sequence similarity (94 to 97%), and 84 to 98% in their N-terminal regions. Furthermore, their 3′-NTRs were very similar in length [253 to 256 nucleotides (nt)] and sequence (93 to 96% similarity). In contrast, the coat protein of NL3 had only 261 amino acids and showed 87 to 89% similarity with NL1, NY15 and W whereas its N-terminal region revealed only 46 to 61% similarity. The 3′-NTR of NL3 also displayed appreciable differences, both in length (240 nt) and sequence (56 to 63% similarity). These results, in combination with earlier serological findings, justify the conclusion that NL1, NY15 and W should be considered strains of the same virus, i.e. bean common mosaic virus, and that NL3 is a strain of a different potyvirus for which the name ‘bean black root virus’ is proposed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-10-2243
1993-10-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/10/JV0740102243.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-10-2243&mimeType=html&fmt=ahah

References

  1. Atreya C. D., Raccah B., Pirone T. P. 1990; A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178:161–165
    [Google Scholar]
  2. Barnett O. W. 1991; Potyviridae, a proposed family of plant viruses. Archives of Virology 118:139–141
    [Google Scholar]
  3. Cassidy B., Sherwood J. L., Nelson R. S. 1993; Cloning of the capsid protein gene from a blotch isolate of peanut stripe virus. Archives of Virology 128:287–293
    [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  5. Dijkstra J., Khan J. A. 1992; A proposal for a bean common mosaic subgroup of potyviruses. Archives of Virology supplement 5389–395
    [Google Scholar]
  6. Drijfhout E. 1978; Genetic interaction between Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. Agricultural Research Reports 872. Pudoc, Wageningen, The Netherlands
    [Google Scholar]
  7. Frenkel M. J., Ward C. W., Shukla D. D. 1989; The use of 3′ non-coding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and soybean mosaic virus-N. Journal of General Virology 70:2775–2783
    [Google Scholar]
  8. Geysen H. M., Meloen R. H., Barteling S. J. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of single amino acids. Proceedings of the National Academy of Sciences, U,. S,. A 81:3998–1002
    [Google Scholar]
  9. Geysen H. M., Rodda S. J., Mason T. J., Tribbick G., Schoofs P. G. 1987; Strategies for epitope analysis using peptide synthesis. Journal of Immunological Methods 102:259–274
    [Google Scholar]
  10. Geysen H. M., Mason T. J., Rodda S. J. 1988; Cognitive features of continuous antigenic determinants. Journal of Molecular Recognition 1:32–42
    [Google Scholar]
  11. Grogan R. G., Walker J. C. 1948; The relation of common mosaic to black root of bean. Journal of Agricultural Research 77:315–331
    [Google Scholar]
  12. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  13. Harrison B. D., Robinson D. J. 1988; Molecular variation in vector-borne plant viruses: epidemiological significance. Philosophical Transactions of the Royal Society of London Series B 321:447–462
    [Google Scholar]
  14. Khan J. A., Lohuis H., Goldbach R. W., Dijkstra J. 1990; Distinction of strains of bean common mosaic virus using antibodies to N- and C-or N-terminal peptide domains of coat protein. Annals of Applied Biology 117:583–593
    [Google Scholar]
  15. Lana A. F., Lohuis H., Bos L., Dijkstra J. 1988; Relationships among strains of bean common mosaic virus and blackeye cowpea mosaic virus members of the potyvirus group. Annals of Applied Biology 113:493–505
    [Google Scholar]
  16. Langeveld S. A., Dore J.-M., Memelink J., Derks A. F. L. M., van der Vlugt C. I. M., Asjes C. J., Bol J. F. 1991; Identification of potyviruses using the polymerase chain reaction with degenerate primers. Journal of General Virology 72:1531–1541
    [Google Scholar]
  17. McKern N. M., Edskes H. K., Ward C. W., Strike P. M., Barnett O. W., Shukla D. D. 1991; Coat protein of potyviruses 7. Amino acid sequence of peanut stripe virus. Archives of Virology 119:25–35
    [Google Scholar]
  18. McKern N. M., Mink G. I., Barnett O. W., Mishra A., Whittaker L. A., Silbernagel M. J., Ward C. W., Shukla D. D. 1992a; Isolates of bean common mosaic virus comprising two distinct potyviruses. Phytopathology 82:923–929
    [Google Scholar]
  19. McKern N. M., Ward C. W., Shukla D. D. 1992b; Strains of bean common mosaic virus consists of at least two distinct potyviruses. Archives of Virology supplement 5407–414
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Marchuk D., Drumm M., Saulino A., Collins F. S. 1990; Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Research 19:1154
    [Google Scholar]
  22. Mink G. I., Silbernagel M. J. 1992; Serological and biological relationships among viruses in the bean common mosaic virus subgroup. Archives of Virology supplement 5397–406
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A 74:5463–5467
    [Google Scholar]
  24. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  25. Shukla D. D., Ward C. W. 1989; Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Archives of Virology 106:171–200
    [Google Scholar]
  26. Shukla D. D., Jilka J., Tosic M., Ford R. E. 1989; A novel approach to the serology of potyviruses involving affinity-purified polyclonal antibodies directed towards virus-specific N termini of coat proteins. Journal of General Virology 70:13–23
    [Google Scholar]
  27. Shukla D. D., Lauricella R., Ward C. W. 1992; Serology of potyviruses: current problems and some solutions. Archives of Virology supplement 5:57–69
    [Google Scholar]
  28. Tautz D., Renz M. 1983; An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 132:14–19
    [Google Scholar]
  29. Vetten H. J., Lesemann D.-E., Maiss E. 1992; Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. Archives of Virology supplement 5415–431
    [Google Scholar]
  30. Ward C. W., McKern N. M., Frenkel M. J., Shukla D. D. 1992; Sequence data as the major criterion for potyvirus classification. Archives of Virology supplement 5283–297
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-10-2243
Loading
/content/journal/jgv/10.1099/0022-1317-74-10-2243
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error