Deleting two amino acids in glycoprotein gI of pseudorabies virus decreases virulence and neurotropism for pigs, but does not affect immunogenicity Free

Abstract

The virulence, pathogenicity and immunogenicity of two pseudorabies virus (PRV) variants were investigated in 3-week-old pigs that had been intranasally infected. Variant M303 (Δ125,126) lacked amino acids valine (125) and cysteine(126) in an immunodominant antigenic region of glycoprotein I (gI) containing two discontinuous antigenic domains, whereas M304 (Δ59,60) lacked amino acids glycine(59) and aspartic acid(60) in a continuous antigenic domain. M303 (Δ125,126) was not virulent for pigs, but M304 (Δ59,60) was as virulent as wild-type PRV: all pigs died within 8 days of infection. Both gI mutant viruses replicated in the oropharyngeal mucosa, although M304 (Δ59,60) replicated to higher virus titres than M303 (Δ125,126), and virus was recovered from various tissues. However, in contrast to M304 (Δ59,60), M303 (Δ125,126) was not recovered from any central nervous system (CNS) tissues examined. Thus, the tendency of PRV to locate in the CNS was markedly reduced by deleting amino acids valine(125) and cysteine(126) of gI. Pigs immunized with M303 were completely protected against challenge infection; no clinical signs of disease were detected, no virus was shed, and no secondary antibody response was detected. Thus, deleting amino acids valine(125) and cysteine(126) in gI decreases virulence and neurotropism and does not affect immunogenicity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-10-2201
1993-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/10/JV0740102201.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-10-2201&mimeType=html&fmt=ahah

References

  1. Ben-Porat T., DeMarchi J., Pendrys J., Veach A., Kaplan A. S. 1986; Proteins specified by the short unique region of the genome of pseudorabies virus play a role in the release of virions from certain cells. Journal of Virology 57:191–196
    [Google Scholar]
  2. Berns A., van de Ouweland A., Quint W., Van Oirschot J., Gielkens A. 1985; Presence of markers in the unique short region or repeat region or both of pseudorabies virus. Journal of Virology 53:89–93
    [Google Scholar]
  3. Bitsch V., Eskildsen M. 1976; A comparative examination of swine sera for antibody to Aujeszky virus with the conventional and a modified virus-serum neutralization test and a modified complement fixation test. Acta veterinaria scandinavica 17:142–145
    [Google Scholar]
  4. Card J. P., Whealy M. E., Robbins A. K., Moore R. Y., Enquist L. W. 1991; Two different a-herpesvirus strains are transported differentially in the rodent system. Neuron 6:957–968
    [Google Scholar]
  5. Card J. P., Whealy M. E., Robbins A. K., Enquist L. W. 1992; Pseudorabies virus envelope glycoprotein gI influences both neurotropism and virulence during infection of the rat visual system. Journal of Virology 66:3032–3041
    [Google Scholar]
  6. De Leeuw P. W., Van Oirschot J. T. 1985; Vaccines against Aujeszky’s disease: evaluation of their efficacy under standardized laboratory conditions. Veterinary Quarterly 7:191–197
    [Google Scholar]
  7. Jacobs L., Meloen R. H., Gielkens A. L. J., Van Oirschot J. T. 1990; Epitope analysis of glycoprotein I of pseudorabies virus. Journal of General Virology 71:881–887
    [Google Scholar]
  8. Jacobs L., Rziha H.-J., Kimman T. G., Gielkens A. L. J., Van Oirschot T. J. 1993; Deleting valine-125 and cysteine-126 in glycoprotein gI of pseudorabies virus strain NIA-3 decreases plaque size and reduces virulence for mice. Archives of Virology (in press)
    [Google Scholar]
  9. Kimman T. G., De Wind N., Oei-Lie N., Pol J. M. A., Berns A. J. M., Gielkens A. L. J. 1992; Contribution of single genes within the unique short region of Aujeszky’s disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. Journal of General Virology 73:243–251
    [Google Scholar]
  10. Kuypers H. G. J. M., Ugolini G. 1990; Virus as transneural tracers. Topics in Neuroscience 13:71–75
    [Google Scholar]
  11. Lafay F., Coulon P., Astic L., Saucier D., Riche D., Holley A., Flamand A. 1991; Spread of the CVS strain of rabies virus and of the avirulent mutant Av01 along the olfactory pathways of the mouse after intranasal inoculation. Virology 183:320–330
    [Google Scholar]
  12. Lomniczi B., Watanabe S., Ben-Porat T., Kaplan A. S. 1984; Genetic basis of the neurovirulence virus. Journal of Virology 52:198–205
    [Google Scholar]
  13. Lomniczi B., Watanabe S., Ben-Porat T., Kaplan A. S. 1987; Genome location and identification of functions defective in the Bartha vaccine strain of pseudorabies virus. Journal of Virology 61:796–801
    [Google Scholar]
  14. McFerran J. B., Dow C. 1975; Studies on immunisation of pigs with the Bartha strain of Aujeszky’s disease virus. Research in Veterinary Science 19:17–22
    [Google Scholar]
  15. Mengeling W. L. 1991; Virus reactivation in pigs latently infected with a thymidine kinase negative vaccine strain of pseudorabies virus. Archives of Virology 120:57–70
    [Google Scholar]
  16. Mettenleiter T. C. 1991; Molecular biology of pseudorabies (Aujeszky’s disease) virus. Comparative Immunology, Microbiology and Infectious Diseases 14:151–163
    [Google Scholar]
  17. Mettenleiter T. C., Lukacs N., Rziha H.-J. 1985; Pseudorabies virus avirulent strains fail to express a major glycoprotein. Journal of Virology 56:307–311
    [Google Scholar]
  18. Mettenleiter T. C., Zsak L., Kaplan A. S., Ben-Porat T., Lomniczi B. 1987; Role of a structural glycoprotein of pseudorabies virus in virus virulence. Journal of Virology 61:4030–4032
    [Google Scholar]
  19. Pol J. M. A., Quint W. G. V., Kok G. L., Broekhuysen-Davis J. M. 1991; Pseudorabies virus infection in explants of porcine nasal mucosa. Research in Veterinary Science 50:45–53
    [Google Scholar]
  20. Strack A. M., Loewy A. D. 1990; Pseudorabies virus: a highly specific transneural cell body marker in the sympathetic nervous system. Journal of Neuroscience 7:2139–2147
    [Google Scholar]
  21. Tuffereau C., Leblois H., Benejan J., Coulon P., Lafay F., Flamand A. 1989; Arginine or lysine in position 333 of ERA and CVS glycoprotein is necessary for rabies virulence in adult mice. Virology 172:206–212
    [Google Scholar]
  22. Van Oirschot J. T. 1989; The antibody response to glycoprotein I and the control of Aujeszky’s disease. In Vaccination and Control of Aujeszky’s Disease CEC Seminar pp 129–138 Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  23. Van Oirschot J. T., Houwers D. J., Rziha H.-J., van Zaane D. 1988; Development of an ELISA for detection of antibodies to glycoprotein I of Aujeszky’s disease virus: a method for the serological differentiation between infected and vaccinated pigs. Journal of Virological Methods 22:191–206
    [Google Scholar]
  24. Van Oirschot J. T., Gielkens A. L. J., Moormann R. J. M., Berns A. J. M. 1990; Marker vaccines, virus protein-specific antibody assays and the control of Aujeszky’s disease. Veterinary Microbiology 23:85–101
    [Google Scholar]
  25. Westrop G. D., Wareham K. A., Evans M. A., Dunn G., Minor P. D., Magrath D. I., Taffs F., Marsden S., Skinner M. A., Schild G. C., Almond J. W. 1989; Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine. Journal of Virology 63:1338–1344
    [Google Scholar]
  26. Zuckermann F. A., Mettenleiter T. C., Scheurs C. A., Sugg N., Ben-Porat T. 1988; Complex between glycoprotein gI and gp63 of pseudorabies virus: its effect on virus replication. Journal of Virology 62:4622–4626
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-10-2201
Loading
/content/journal/jgv/10.1099/0022-1317-74-10-2201
Loading

Data & Media loading...

Most cited Most Cited RSS feed