1887

Abstract

The physical and biological state of the Marek’s disease virus (MDV) genome in avian leukosis virus (ALV)-transformed cells is characterized using cell lines established from ALV tumours co-infected with the SB-1 strain of MDV. The MDV genome within the ALV-transformed cells was found to be methylated at 5′ CpG 3′ dinucleotides. Less than 2% of the tumour cells expressed MDV antigen and only one virus plaque that was characteristic of an MDV infection was noted when tumour cells were cocultured with fibroblasts permissive for a productive MDV infection. However, when methylation of the MDV genome was prevented by culturing the tumour cell lines in the presence of 5-azacytidine, both MDV antigen expression and viral replication increased. Based on these results, it appears that MDV resides within the ALV-transformed cells in a latent state and that MDV latency might be influenced, to some extent, by methylation of the MDV genome.

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1993-10-01
2021-10-25
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1987 Current Protocols in Molecular Biology New York: John Wiley and Sons;
    [Google Scholar]
  2. Bacon L. D., Witter R. L., Fadly A. M. 1989; Augmentation of retrovirus-induced lymphoid leukosis by Marek’s disease herpesviruses in White Leghorn chickens. Journal of Virology 63:504–512
    [Google Scholar]
  3. Ben-Sasson S. A., Klein G. 1981; Activation of the Epstein-Barr virus genome by 5-azacytidine in latently infected human lymphoid lines. International Journal of Cancer 28:131–135
    [Google Scholar]
  4. Carter J. K., Silva R. F. 1990; Cell culture amplification of a defective Marek’s disease virus. Virus Genes 4:225–237
    [Google Scholar]
  5. Desrosiers R. C., Mulder C., Fleckenstein B. 1979; Methylation of herpesvirus saimiri DNA in lymphoid tumor cell lines. Proceedings of the National Academy of Sciences, U.S.A 76:3839–3843
    [Google Scholar]
  6. Doerfler W. 1982; DNA methylation and gene activity. Annual Review of Biochemistry 52:93–124
    [Google Scholar]
  7. Ehrlich M., Wang R. Y.-H. 1981; 5-Methylcytosine in eukaryotic DNA. Science 212:1350–1357
    [Google Scholar]
  8. Ewert D. L., De Boer G. F. 1988; Avian lymphoid leukosis: mechanisms of lymphomagenesis. Advances in Veterinary Science and Comparative Medicine 32:37–55
    [Google Scholar]
  9. Fynan E. F., Block T. M., DuHadaway J., Olson W., Ewert D. L. 1992; Persistence of Marek’s disease virus in a subpopulation of B cells that is transformed by avian leukosis virus, but not in normal bursal B cells. Journal of Virology 66:5860–5866
    [Google Scholar]
  10. Groudine M., Eisenman R., Weintraub H. 1981; Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature, London 292:311–317
    [Google Scholar]
  11. Hoffman J. W., Steffen D., Gusella J., Tabin C., Bird S., Cowing D., Weinberg R. A. 1982; DNA methylation affecting the expression of murine leukemia proviruses. Journal of Virology 44:144–157
    [Google Scholar]
  12. Hsuing G. D. 1982; Cell culture preparation. In Diagnostic Virology pp 249–250 New Haven: Yale University Press;
    [Google Scholar]
  13. Kanamori A., Ikuta K., Ueda S., Kato S., Hirai K. 1987; Methylation of Marek’s disease virus DNA in chicken T-lymphoblastoid cell lines. Journal of General Virology 68:1485–1490
    [Google Scholar]
  14. Lee L. F., Liu X., Witter R. L. 1983; Monoclonal antibodies with specificity for three different serotypes of Marek’s disease viruses in chickens. Journal of Immunology 130:1003–1006
    [Google Scholar]
  15. Minarovits J., Minarovits-Kormuta S., Ehlin-Henriksson B., Falk K., Klein G., Ernberg I. 1991; Host cell phenotype-dependent methylation patterns of Epstein-Barr virus DNA. Journal of General Virology 12:1591–1599
    [Google Scholar]
  16. Niwa O., Sughara T. 1981; 5-Azacytidine induction of mouse endogenous type C virus and suppression of DNA methylation. Proceedings of the National Academy of Sciences, U.S.A 78:6290–6294
    [Google Scholar]
  17. Payne L. N. 1982; Biology of Marek’s disease virus and the herpesvirus of turkeys. In The Herpesviruses vol 1 pp 347–431 Edited by Roizman B. New York: Plenum Press;
    [Google Scholar]
  18. Purchase H. G. 1986; The pathogenesis and pathology of neoplasms caused by avian leukosis viruses. In Avian Leukosis pp 171–196 Edited by DeBoer G. F. Boston: Martinus Nijhoff Publishing;
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Flarbor Laboratory;
  20. Schat K. A., Calnek B. W. 1978; Characterization of an apparently nononcogenic Marek’s disease virus. Journal of the National Cancer Institute 60:1075–1081
    [Google Scholar]
  21. Taylor S. M., Constantinides P. A., Jones P. A. 1984; 5-Azacytidine, DNA methylation, and differentiation. Current Topics in Microbiology and Immunology 108:115–127
    [Google Scholar]
  22. Waalwijk C., Flavell R. A. 1978; Msp I, an isoschizomer of Hpa II which cleaves both unmethylated and methylated Hpa II sites. Nucleic Acids Research 5:3231–3236
    [Google Scholar]
  23. Youssoufian H., Hammer S. M., Hirsch M. S., Mulder C. 1982; Methylation of the viral genome in an in vitro model of herpes simplex virus latency. Proceedings of the National Academy of Sciences, U.S.A. 79:2207–2210
    [Google Scholar]
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