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Journal of General Virology header logo Journal of General Virology

Volume 74, Issue 10

Epitope mapping of envelope glycoprotein E1 of hog cholera virus strain Brescia No Access

Abstract

Four antigenic domains (A, B, C and D) on envelope glycoprotein E1 (gp51-54) of hog cholera virus strain Brescia have been specified by using 13 monoclonal antibodies (MAbs) that recognize non-conserved and conserved epitopes. It was shown that the non-conserved epitopes map to the N-terminal half of E1 by analysis of chimeric E1 proteins of strains Brescia and C. Conserved epitopes, however, could not be mapped using this approach. Here we describe mapping of both conserved and non-conserved epitopes on E1 by the use of an extensive set of single and double deletion mutants of E1 of strain Brescia. Deletion mutants were transiently expressed in COS1 cells and analysed by immuno-staining with the 13 MAbs directed against strain Brescia and four MAbs directed against strain C. All MAbs bound to the N-terminal half of E1, i.e. amino acids 690 to 866 encoded by the sequence of strain Brescia. Domain B and one epitope in domain C are located between residues 690 and 773. Other epitopes in domain C are located on an extended region, i.e. between residues 690 and 800. Conserved epitopes of domain A are mapped between residues 766 and 866, whereas the only non-conserved epitope in this domain is located between residues 766 and 813. Domain D, represented by one MAb, is located in the same region as this non-conserved epitope of domain A, i.e. between residues 766 and 800. The results suggest the presence of two distinct antigenic units on E1, one consisting of domains B and C and the other consisting of domain A.

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© Journal of General Virology 1993
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1993-10-01
2025-01-13
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References

  1. Bognár K., Mészáros J. 1963; Experiences with lapinised hog cholera virus strain of decreased virulence. Acta veterinaria Acade-miae scientiarum hungaricae 13:429–138
     [Google Scholar]
  2. Cohen G. H., Wilcox W. C., Sodora D. L., Long D., Levin J. Z., Eisenberg R. J. 1988; Expression of herpes simplex virus type 1 glycoprotein D deletion mutants in mammalian cells. Journal of Virology 62:1932–1940
     [Google Scholar]
  3. Collett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. 1988a; Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165:191–199
     [Google Scholar]
  4. Collett M. S., Larson R., Belzer S. K., Retzel E. 1988b; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165:200–208
     [Google Scholar]
  5. Deng R., Brock K. V. 1992; Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1. Virology 191:867–879
     [Google Scholar]
  6. Eisenberg D., Schwartz E., Komarony M., Wall R. 1984; Analysis of membrane and surface protein sequences with the hydrophobic moment plot. Journal of Molecular Biology 179:125–142
     [Google Scholar]
  7. Francki R. I. B., Fauqubt C. M., Knudson D. L., Brown F. 1991; Fifth Report of the International Committee on the Taxonomy of Viruses. Archives of Virology Supplementum 2223–233
     [Google Scholar]
  8. Geysen H. M., Meloen R. H., Barteling S. J. 1984; Use of peptide synthesis to probe viral antigen for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, U,. S,. A 81:3998–1002
     [Google Scholar]
  9. Hanahan D. 1985; Techniques for transformation of E. coli . In DNA Cloning: A Practical Approach vol 1 pp 109–135 Edited by Glover D. M. Oxford: IRL Press;
     [Google Scholar]
  10. Meyers G., Rumenapf T., Thiel H.-J. 1989; Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171:555–567
     [Google Scholar]
  11. Moormann R. J. M., Hulst M. M. 1988; Hog cholera virus: identification and characterization of the viral RNA and virus-specific RNA synthesized in infected swine kidney cells. Virus Research 11:281–291
     [Google Scholar]
  12. Moormann R. J. M., Warmerdam P. A., van der Meer B., Schaaper W. M. M., Wensvoort G., Hulst M. M. 1990a; Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein El. Virology 177:184–198
     [Google Scholar]
  13. Moormann R. J. M., Warmerdam P. A., van der Meer B., Hulst M. M. 1990b; Nucleotide sequence of hog cholera virus RNA: properties of the polyprotein encoded by the open reading frame spanning the viral genomic RNA. Veterinary Microbiology 23:185–191
     [Google Scholar]
  14. Paton D. J., Lowings J. P., Barrett A. D. T. 1992; Epitope mapping of the gp53 envelope protein of bovine viral diarrhea virus. Virology 190:763–772
     [Google Scholar]
  15. Peeters B., de Wind N., Hooisma M., Wagenaar F., Gielkens A., Moormann R. 1992; Pseudorabies virus envelope glycoproteins gp50 and gll are essential for virus penetration, but only gll is involved in membrane fusion. Journal of Virology 66:894–905
     [Google Scholar]
  16. Rao M. J. K., Argos P. 1986; Prediction of protein structure. Biochimica et biophysica acta 869:197–214
     [Google Scholar]
  17. Renard A., Guiot C., Schmetz D., Dagenais L., Pastoret P.-P., Dina D., Martial J. A. 1985; Molecular cloning of bovine viral diarrhea virus sequences. DNA 4:429–438
     [Google Scholar]
  18. Renard A., Dina D., Martial J. A. 1987; Complete nucleotide sequence of bovine viral diarrhoea virus genome and its fragment, useful for making antigenic proteins in therapy and diagnosis. European Patent Application no. 0208672
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  20. Stark R., Rumenapf T., Meyers G., Thiel H.-J. 1990; Genomic localization of hog cholera virus glycoproteins. Virology 174:286–289
     [Google Scholar]
  21. Thiel H.-J., Stark R., Weiland E., Rumenapf T., Meyers G. 1991; Hog cholera virus: molecular composition of virions from a pestivirus. Journal of Virology 65:4705–4712
     [Google Scholar]
  22. van Rijn P. A., van Gennip H. G. P., de Meijer E. J., Moormann R. J. M. 1992; A preliminary map of epitopes on envelope glycoprotein El of HCV strain Brescia. Veterinary Microbiology 33:221–230
     [Google Scholar]
  23. van Zijl M., Wensvoort G., De Kluyver E., Hulst M. M., van Der Gulden H., Gielkens A. L. J., Berns A., Moormann R. J. M. 1991; Live attenuated pseudorabies virus expressing envelope glycoprotein El of hog cholera virus protects swine against both pseudorabies and hog cholera. Journal of Virology 65:2761–2765
     [Google Scholar]
  24. Weiland E., Stark R., Haas B., Rumenapf T., Meyers G., Thiel H.-J. 1990; Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer. Journal of Virology 64:3563–3569
     [Google Scholar]
  25. Weiland E., Ahl R., Stark R., Weiland F., Thiel H.-J. 1992; A second envelope glycoprotein mediates neutralization of a pestivirus, hog cholera virus. Journal of Virology 66:3677–3682
     [Google Scholar]
  26. Wensvoort G. 1989a Epitopes on structural proteins of hog cholera (swine fever) virus Ph. D. thesis State University of Utrecht:
     [Google Scholar]
  27. Wensvoort G. 1989b; Topographical and functional mapping of epitopes on hog cholera virus with monoclonal antibodies. Journal of General Virology 70:2865–2876
     [Google Scholar]
  28. Wensvoort G., Terpstra C., Boonstra J., Bloemraad M., van Zaane D. 1986; Production of monoclonal antibodies against swine fever and their use in laboratory diagnosis. Veterinary Microbiology 12:101–108
     [Google Scholar]
  29. Wensvoort G., Terpstra C., de Kluijver E. J., Kragten C., Warnaar J. C. 1989; Antigenic differentiation of pestivirus strains with monoclonal antibodies against hog cholera virus. Veterinary Microbiology 21:9–20
     [Google Scholar]
  30. Wensvoort G., Boonstra J., Bodzinga B. G. 1990; Itnmuno-affinity purification and characterization of the envelope protein El of hog cholera virus. Journal of General Virology 71:531–540
     [Google Scholar]
  31. Xue W., Minocha H. C. 1993; Identification of the cell surface receptor for bovine viral diarrhoea virus by using anti-idiotypic antibodies. Journal of General Virology 74:73–79
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-10-2053
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Epitope mapping of envelope glycoprotein E1 of hog cholera virus strain Brescia
J. Gen. Virol. 74, 2053 (1993); https://doi.org/10.1099/0022-1317-74-10-2053
/content/journal/jgv/10.1099/0022-1317-74-10-2053
/content/journal/jgv/10.1099/0022-1317-74-10-2053
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