1887

Abstract

Obtaining an isolate of a human influenza virus in the allantoic cavity of the embryonated hen’s egg is more efficient if the clinical sample is initially passaged in the amniotic cavity. To investigate the extent to which the variants present after allantoic propagation are also selected by amniotic passage, clinical virus passaged once in the amnion has been subjected to extensive genetic and antigenic analyses. The data indicate that the natural virus can replicate unrestrictedly within the amnion. However, exposure of amniotic virus to the allantois during the incubation period, which will occur through the hole between the amniotic and allantoic cavities caused by the inoculating needle, allows for the possibility of an egg-adapted variant establishing replication within the allantois and returning to the amnion. These observations illustrate why prior passage in the amnion increases the probability of a variant successfully establishing itself during a subsequent allantoic passage.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-10-2047
1993-10-01
2024-06-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/10/JV0740102047.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-10-2047&mimeType=html&fmt=ahah

References

  1. Burnet F. M. 1940; Influenza virus infections of the chick embryo lung. British Journal of Experimental Pathology 21:147–153
    [Google Scholar]
  2. Burnet F. M., Bull D. R. 1943; Changes in influenza virus associated with adaptation to passage in chick embryos. Australian Journal of Experimental Biology and Medical Science 21:55–69
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Hoyle L. 1968 The Influenza Viruses pp 25–27 Wien: Springer–Verlag;
    [Google Scholar]
  5. Katz J. M., Webster R. G. 1988; Antigenic and structural characterization of multiple subpopulations of H3N2 influenza virus from an individual. Virology 165:446–456
    [Google Scholar]
  6. Katz J. M., Webster R. G. 1992; Amino acid sequence identity between the HA1 of influenza A (H3N2) viruses grown in mammalian and primary chick kidney cells. Journal of General Virology 73:1159–1165
    [Google Scholar]
  7. Katz J. M., Naeve C. W., Webster R. G. 1987; Host cell-mediated variation in H3N2 influenza viruses. Virology 156:386–395
    [Google Scholar]
  8. Katz J. M., Wang M., Webster R. G. 1990; Direct sequencing of the HA gene of influenza (H3N2) virus in original clinical samples reveals sequence identity with mammalian cell-grown virus. Journal of Virology 64:1808–1811
    [Google Scholar]
  9. Robertson J. S., Naeve C. W., Webster R. G., Bootman J. S., Newman R., Schild G. C. 1985; Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs. Virology 143:166–174
    [Google Scholar]
  10. Robertson J. S., Bootman J. S., Newman R., Oxford J. S., Daniels R. S., Webster R. G., Schild G. C. 1987; Structural changes in the hemagglutinin which accompany egg adaptation of an influenza A (H1N1) virus. Virology 160:31–37
    [Google Scholar]
  11. Robertson J. S., Bootman J. S., Nicolson C., Major D., Robertson E. W., Wood J. M. 1990; The hemagglutinin of influenza B virus present in clinical material is a single species identical to that of mammalian cell-grown virus. Virology 179:35–40
    [Google Scholar]
  12. Robertson J. S., Nicolson C., Bootman J. S., Major D., Robertson E. W., Wood J. M. 1991; Sequence analysis of the haemagglutinin (HA) of influenza A (H1N1) viruses present in clinical material and comparison with the HA of laboratory-derived virus. Journal of General Virology 72:2671–2677
    [Google Scholar]
  13. Rota P. A., Hemphill M. L., Whistler T., Regnery H. L., Kendal A. P. 1992; Antigenic and genetic characterization of the haemagglutinins of recent cocirculating strains of influenza B virus. Journal of General Virology 73:2737–2742
    [Google Scholar]
  14. Schild G. C., Oxford J. S., de Jong J. C., Webster R. W. 1983; Evidence for host cell selection of influenza virus antigenic variants. Nature, London 303:706–709
    [Google Scholar]
  15. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-10-2047
Loading
/content/journal/jgv/10.1099/0022-1317-74-10-2047
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error