1887

Abstract

A cDNA encoding the cytoplasmic polyhedrin of cytoplasmic polyhedrosis virus (CPV) strain H was introduced into an improved baculovirus expression vector which can be utilized to express foreign genes in the cell line IPLB-SF-21AE (Sf21 cells) and the cell line BmN. A recombinant virus produced large, cubic inclusion body-like structures in infected Sf21 and BmN cells. Western blot analysis showed that these structures were CPV polyhedra. This result suggested that the supramolecular assembly of CPV polyhedrin is responsible for its properties.

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1993-01-01
2021-10-21
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