1887

Abstract

We have produced and characterized polyclonal anti-idiotypic antibodies (anti-ids) that mimic the antigenic structures of gp53 from bovine viral diarrhoea virus (BVDV). In this study, the anti-ids were used to identify cell receptors for BVDV. The anti-ids bound specifically to bovine cells, as determined by flow cytometric analysis, and inhibitory binding assays showed that they bound to the cell surface receptors for BVDV. A cell surface protein with an of approximately 50K was immunoprecipitated by the anti-ids from MDBK cells; this was blocked by preincubation of cell lysate with BVDV. This indicates that the 50K protein might be a specific receptor for BVDV gp53. Thirteen BVDV strains were used to evaluate inhibition of anti-id binding to MDBK cells and inhibition of BVDV infection of anti-id-treated MDBK cell monolayers. Results demonstrated that both processes were inhibited to varying degrees depending on virus strain. The results suggested that multiple receptors for BVDV attachment may exist on MDBK cells, and that different virus strains do not have the same receptor.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-1-73
1993-01-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/1/JV0740010073.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-1-73&mimeType=html&fmt=ahah

References

  1. Baker JC. 1987; Bovine viral diarrhea virus: a review. Journal of the American Veterinary Medical Association 190:1449–1458
    [Google Scholar]
  2. Bissey LL, William AK, Bolin S, Collisson EW. 1991; Comparison of cytopathic and noncytopathic isolates of bovine viral diarrhea virus by oligonucleotide fingerprinting. Journal of Veterinary Diagnostic Investigation 3:16–21
    [Google Scholar]
  3. Bolin S, Moennig V, Gourley NEK, Ridpath J. 1988; Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Archives of Virology 99:117–123
    [Google Scholar]
  4. Co M, Gaulton GN, Fields BN, Greene MI. 1985; Isolation and characterization of the mammalian reovirus type 3 cell surface receptor. Proceedings of the National Academy of Sciences, U.S.A. 82:1494–1498
    [Google Scholar]
  5. Collett MS, Larson R, Belzer SK, Petzel E. 1988; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165:200–208
    [Google Scholar]
  6. Collett MS, Moennig V, Horzinek MC. 1989; Recent advances in pestivirus research. Journal of General Virology 70:253–266
    [Google Scholar]
  7. Corapi WV, Donis RO, Dubovi EJ. 1988; Monoclonal antibody analysis of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infection. Journal of Virology 62:2823–2827
    [Google Scholar]
  8. Corapi WV, Donis RO, Dubovi EJ. 1990; Characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. American Journal of Veterinary Research 51:1388–1394
    [Google Scholar]
  9. Donis RO, Corapi W, Dubovi EJ. 1988; Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56K to 58K glycoprotein. Journal of General Virology 69:77–86
    [Google Scholar]
  10. Grieder FB, Schultz KT. 1990; Antiidiotypic antibody mimicry of a bluetongue virus neutralizing antigen. Journal of Immunology 144:2627–2631
    [Google Scholar]
  11. Homey CJ, Rockson SG, Haber E. 1982; An anti-idiotypic antibody that recognizes the β-adrenergic receptor. Journal of Clinical Investigation 69:1147–1156
    [Google Scholar]
  12. Jewett CT, Kelling CK, Frey ML, Doster AR. 1990; Comparative pathogenicity of selected bovine viral diarrhea virus isolates in gnotobiotic lambs. American Journal of Veterinary Research 51:1640–1646
    [Google Scholar]
  13. Keay S, Merigan TC, Rasmussen L. 1989; Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus. Proceedings of National Academy of Sciences, U.S.A. 86:10100–10103
    [Google Scholar]
  14. Kelling CL, Kennedy JE, Stine LC, Rump KK, Paul PS, Partridge JE. 1990; Genetic comparison of ovine and bovine pestiviruses. American Journal of Veterinary Research 51:2019–2024
    [Google Scholar]
  15. Krah DL, CHOPPIN PW. 1988; Mice immunized with measles virus develop antibodies to a cell surface receptor for binding virus. Journal of Virology 62:1565–1572
    [Google Scholar]
  16. Laemmli UK. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  17. Magar R, Minocha HC, Lecomte J. 1988a; Bovine viral diarrhea virus proteins: heterogeneity of cytopathogenic and noncytopathogenic strains and evidence of 53K glycoprotein neutralization epitope. Veterinary Microbiology 16:303–314
    [Google Scholar]
  18. Magar R, Minocha HC, Carman PS, Lecomte J. 1988b; Typing of cytopathic and noncytopathic bovine viral diarrhea virus references and Canadian field strains using a neutralizing monoclonal antibody. Canadian Journal of Veterinary Research 52:42–45
    [Google Scholar]
  19. Marriott SJ, Roeder DJ, Consigli R. 1987; Anti-idiotypic antibodies to a polyomavirus monoclonal antibody recognize cell surface components of mouse kidney cells and prevent polyomavirus infection. Journal of Virology 61:2747–2753
    [Google Scholar]
  20. Mendelsohn C, Wimmer E, Racaniello VR. 1989; Cellular receptor for poliovirus: molecular cloning, nucleotide sequence and expression of a new member of the immunoglobulin superfamily. Cell 56:855–865
    [Google Scholar]
  21. Moennig V, Bolin RS, Mateo A, Kelso-Gourley N, Kelso-Gourley NE, Wilke I, Liess B. 1988; Identification of a single receptor for bovine viral diarrhea virus (BVDV) on the surface of cultured cells. Abstracts, Annual Meeting of the American Society for Microbiology, Miami Beach, Florida8–13May p 326
    [Google Scholar]
  22. Perdrizet J, Rebhun WC, Dubovi EJ, Donis RO. 1987; Bovine virus diarrhea-clinical syndromes in dairy herds. Cornell Veterinarian 11:46–74
    [Google Scholar]
  23. Reagan KJ, Goldberg B, Crowell RL. 1984; Altered receptor specificity of coxsackievirus B3 after growth in rhabdomyosarcoma cells. Journal of Virology 49:635–640
    [Google Scholar]
  24. Ridpath JF, Lewis TL, Bolin SR, Berry ES. 1991; Antigenic and genomic comparison between non-cytopathic and cytopathic bovine viral diarrhoea viruses isolated from cattle that had spontaneous mucosal disease. Journal of General Virology 72:725–729
    [Google Scholar]
  25. Schreiber AB, Cournand PO, Andre C, Vray B, Strosberg AD. 1980; Anti-alprenolol anti-idiotypic antibodies bind to β-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase.. Proceedings of the National Academy of Sciences, V.S.A. 11:7385–7389
    [Google Scholar]
  26. Sege K, Peterson PA. 1978; Use of anti-idiotypic antibodies as cell surface receptor probes. Proceedings of the National Academy of Sciences, U.S.A. 75:2443–2447
    [Google Scholar]
  27. Teyssedou E, Magar R, Justewicz DM, Lecomte J. 1987; Cell-protective monoclonal antibodies to bovine enterovirus-3 and partial or no activity against other serotype. Journal of Virology 61:2050–2053
    [Google Scholar]
  28. Xue W, Blecha F, Minocha HC. 1990; Antigenic variations in bovine viral diarrhea viruses detected by monoclonal antibodies. Journal of Clinical Microbiology 28:1688–1693
    [Google Scholar]
  29. Xue W, Orten DJ, Abdelmagid OY, Rider M, Blecha F, Minocha HC. 1991; Anti-idiotypic antibodies mimic bovine viral diarrhea virus neutralizing antigens. Veterinary Microbiology 29:201–212
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-1-73
Loading
/content/journal/jgv/10.1099/0022-1317-74-1-73
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error