Double vaccinia virus recombinants expressing both the T7 RNA polymerase gene, controlled by a weak early poxvirus P promoter, and the β-galactosidase gene, controlled by the phage T7 promoter, have been obtained. The viability of the double recombinants depended on the T7 RNA polymerase expression level. If the T7 RNA polymerase gene was inserted into a recombinant already containing the β-galactosidase gene, the efficiency of formation of the double recombinants was significantly higher compared to that for the reverse insertion order. The negative effect of the phage T7 terminator on β-galactosidase expression in cells infected with the recombinant viruses has been shown. The dynamics and levels of β-galactosidase formation by different vaccinia virus recombinants have been studied.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error