Nucleotide sequence and genome organization of tomato leaf curl geminiviru Free

Abstract

The genome of tomato leaf curl virus (TLCV) from Australia was cloned and its complete nucleotide sequence determined. It is a single circular ssDNA of 2766 nucleotides containing the consensus nonanucleotide sequence present in all geminiviruses. It has six open reading frames with an organization resembling that of certain other dicotyledonous plant-infecting monopartite geminiviruses, i.e. tomato yellow leaf curl and beet curly top viruses. The regulatory sequences present indicate a bidirectional mode of transcription. A dimeric TLCV DNA clone was constructed in a binary vector and used to agroinoculate three different host species. Typical virus infections were produced, confirming that the single DNA component is sufficient for infectivity.

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1993-01-01
2024-03-28
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References

  1. Boulton MI, Steinkellner H, Donson J, Markham PG, King DI, Davies JW. 1989; Mutational analysis of the virion-sense genes of maize streak virus. Journal of General Virology 70:2309–2323
    [Google Scholar]
  2. Breathnach R, Chambon P. 1981; Organization and expression of eukaryotic split genes coding for proteins. Annual Review of Biochemistry 50:349–383
    [Google Scholar]
  3. Coutts RHA, Coffin RS, Roberts EJF, Hamilton WDO. 1991; The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus. Journal of General Virology 72:1515–1520
    [Google Scholar]
  4. Czosnek H, Ber R, Antignus Y, Cohen S, Navot N, Zamir D. 1998; Isolation of tomato leaf curl virus – a geminivirus. Phytopathology 78:508–511
    [Google Scholar]
  5. Davies JW, Stanley J. 1989; Geminivirus genes and vectors. Trends in Genetics 5:77–81
    [Google Scholar]
  6. Ditta G, Stanfield S, Corbin D, Helinski DR. 1980; Broad host range DNA cloning system for Gram-negative bacteria: construction of gene bank of Rhizobium meliloti. Proceedings of the National Academy of Sciences, U.S.A. 77:7341–7351
    [Google Scholar]
  7. Frischmuth T, Zimmat G, Jeske H. 1990; The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–168
    [Google Scholar]
  8. Hamilton WD. O, Stein VE, Coutts RHA, Buck KW. 1984; Complete nucleotide sequence of the infectious cloned DNA components of the tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  9. Holmes DS, Quigley M. 1981; A rapid method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  10. Howarth AJ, Caton J, Bossert J, Goodman RM. 1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings of the National Academy of Sciences, U.S.A. 82:3572–3576
    [Google Scholar]
  11. Khyer-Pour A, Bendahmane M, Matzeit V, Accotto GP, Crespi S, Gronenborn B. 1991; Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucleic Acids Research 19:6763–6769
    [Google Scholar]
  12. MacDowell SW, MacDonald H, Hamilton WDO, Coutts RHA, Buck KW. 1985; The nucleotide sequnce of cloned wheat dwarf virus DNA. EMBO Journal 4:2173–2180
    [Google Scholar]
  13. Makkouk KM, Laterrot H. 1983; Epidemiology and control of tomato yellow leaf curl virus. In Plant Virus Epidemiology: The Spread and Control of Insect-borne Viruses pp 315–321Edited by Plumb RT, Thresh JM. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  14. Morris BAM, Richardson KA, Haley A, Zhan X, Thomas JE. 1992; The nucleotide sequence of the infectious cloned DNA component of tobacco yellow dwarf virus reveals features of geminiviruses infecting monocotyledonous plants. Virology 187:633–642
    [Google Scholar]
  15. Mullineaux PM, Donson J, Morris-Krsinich BAM, Boulton MI, Davies JW. 1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
    [Google Scholar]
  16. Mullineaux PM, Boulton MI, Bowyer P, van der Vlugt R, Marks M, Donson J, Davies JW. 1988; Detection of a nonstructural protein of Mr 11,000 encoded by the virion DNA of maize streak virus. Plant Molecular Biology 11:57–66
    [Google Scholar]
  17. Navot N, Pichersky E, Zeidan M, Zamir D, Czosnek H. 1991; Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. Virology 185:151–161
    [Google Scholar]
  18. Rochester DE, Kositrana W, Beachey RN. 1990; Systemic movement and symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand). Virology 178:520–526
    [Google Scholar]
  19. Sanger F, Nicklen S, Coulson AR. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  20. Stanley J, Gay MR. 1983; Nucleotide sequence of cassava latent virus DNA. Nature, London 301:260–262
    [Google Scholar]
  21. Stanley J, Markham PG, Callis RJ, Pinner MS. 1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
    [Google Scholar]
  22. Thomas JE, Bowyer JW. 1980; Properties of tobacco yellow dwarf and bean summer death viruses. Phytopathology 70:214–217
    [Google Scholar]
  23. Thomas JE, Massalski R, Harrison BD. 1986; Production of monoclonal antibodies to African cassava mosaic virus and differences in their reactivities with other whitefly-transmitted geminiviruses. Journal of General Virology 67:2739–2748
    [Google Scholar]
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