The genome of tomato leaf curl virus (TLCV) from Australia was cloned and its complete nucleotide sequence determined. It is a single circular ssDNA of 2766 nucleotides containing the consensus nonanucleotide sequence present in all geminiviruses. It has six open reading frames with an organization resembling that of certain other dicotyledonous plant-infecting monopartite geminiviruses, i.e. tomato yellow leaf curl and beet curly top viruses. The regulatory sequences present indicate a bidirectional mode of transcription. A dimeric TLCV DNA clone was constructed in a binary vector and used to agroinoculate three different host species. Typical virus infections were produced, confirming that the single DNA component is sufficient for infectivity.
BoultonMI,
SteinkellnerH,
DonsonJ,
MarkhamPG,
KingDI,
DaviesJW.
1989; Mutational analysis of the virion-sense genes of maize streak virus. Journal of General Virology 70:2309–2323
CouttsRHA,
CoffinRS,
RobertsEJF,
HamiltonWDO.
1991; The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus. Journal of General Virology 72:1515–1520
DittaG,
StanfieldS,
CorbinD,
HelinskiDR.
1980; Broad host range DNA cloning system for Gram-negative bacteria: construction of gene bank of Rhizobium meliloti. Proceedings of the National Academy of Sciences, U.S.A. 77:7341–7351
FrischmuthT,
ZimmatG,
JeskeH.
1990; The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–168
HamiltonWD. O,
SteinVE,
CouttsRHA,
BuckKW.
1984; Complete nucleotide sequence of the infectious cloned DNA components of the tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
HowarthAJ,
CatonJ,
BossertJ,
GoodmanRM.
1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings of the National Academy of Sciences, U.S.A. 82:3572–3576
MakkoukKM,
LaterrotH.
1983; Epidemiology and control of tomato yellow leaf curl virus. In Plant Virus Epidemiology: The Spread and Control of Insect-borne Viruses pp 315–321Edited byPlumbRT,
ThreshJM.
Oxford: Blackwell Scientific Publications;
MorrisBAM,
RichardsonKA,
HaleyA,
ZhanX,
ThomasJE.
1992; The nucleotide sequence of the infectious cloned DNA component of tobacco yellow dwarf virus reveals features of geminiviruses infecting monocotyledonous plants. Virology 187:633–642
MullineauxPM,
BoultonMI,
BowyerP,
van der VlugtR,
MarksM,
DonsonJ,
DaviesJW.
1988; Detection of a nonstructural protein of Mr 11,000 encoded by the virion DNA of maize streak virus. Plant Molecular Biology 11:57–66
RochesterDE,
KositranaW,
BeacheyRN.
1990; Systemic movement and symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand). Virology 178:520–526
SangerF,
NicklenS,
CoulsonAR.
1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
StanleyJ,
MarkhamPG,
CallisRJ,
PinnerMS.
1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
ThomasJE,
MassalskiR,
HarrisonBD.
1986; Production of monoclonal antibodies to African cassava mosaic virus and differences in their reactivities with other whitefly-transmitted geminiviruses. Journal of General Virology 67:2739–2748