1887

Abstract

Human immunodeficiency virus type 1 (HIV-1)-infected H9 and blood mononuclear cells (MNCs) were studied by immunogold electron microscopy for the presence of HIV-1 gag p24 protein, env gp41 and gp120 proteins, and host cell molecules CD4, CD11a, CD25, CD54, CD63, HLA class I and HLA-DR. Uninfected H9 cells and MNC membranes labelled for CD4, HLA class I and class II, and, at low density, CD11a and CD54; lysosomal structures in the cytoplasm labelled for CD63. The infected cell surface showed immunolabelling for HIV-1 proteins, as did budding particle-like structures. Immunogold labelling of the cell membrane for CD4 was almost non-existent. The level of immunolabelling for CD11a and CD54 on infected cells was greater than that on uninfected cells; this is presumably related to a state of activation during virus synthesis. Budding particle-like structures and free virions in the intercellular space were immunogold-labelled for all host cell markers investigated. This was confirmed by double immunogold labelling using combinations of HIV-1 gag p24 labelling and labelling for the respective host cell molecule. We conclude that virions generated in HIV-1-infected cells concentrate host-derived molecules on their envelope. Also molecules with a prime function in cellular adhesion concentrate on the virion.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-1-129
1993-01-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/1/JV0740010129.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-1-129&mimeType=html&fmt=ahah

References

  1. Ahsmann EJM, Lokhorst HM, Dekker AW, Bloem Ac. 1992; Lymphocyte function-associated antigen-1 expression on plasma cells correlates with tumor growth in multiple myeloma. Blood 79:2068–2075
    [Google Scholar]
  2. Bloemen P, Moldenhauer G, van Duk M, Schuurman HJ, Bloem AC. 1992; Multiple ICAM-1 (CD54) epitopes are involved in homotypic B-cell adhesion. Scandinavian Journal of Immunology 35:517–523
    [Google Scholar]
  3. Calafat J, Janssen H, Demant P, Hilgers J, Zavada J. 1983; pecific selection of host cell glycoproteins during assembly of murine leukaemia virus and vesicular stomatitis virus: presence of Thy-1 glycoprotein and absence of H-2, Pgp-1 and T-200 glycoproteins on the envelopes of the virus particles. Journal of General Virology 64:1241–1253
    [Google Scholar]
  4. Chen S, Lilly F. 1979; Detection of H-2D antigen on osmotic shock-treated Friend leukemia virus particles. Immunogenetics 9:25–31
    [Google Scholar]
  5. Dustin ML, Springer TA. 1991; The role of lymphocyte adhesion receptors in transient interactions and cell locomotion. Annual Review of Immunology 9:27–66
    [Google Scholar]
  6. Eales LJ, Farrant J, Helbert M, Pinching AJ. 1988; Peripheral blood dendritic cells in persons with AIDS and AIDS related complex: loss of high intensity class II antigen expression and function. Clinical and Experimental Immunology 71:423–127
    [Google Scholar]
  7. Gelderblom HR, Hausmann EHS, Özel M, Pauli G, Koch M. 1987a; Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 156:171–176
    [Google Scholar]
  8. Gelderblom H, Reupke H, Winkel T, Kunze R, Pauli G. 1987b; MHC-antigens: constituents of the envelopes of human and simian immunodeficiency viruses. Zeitschrift fur Naturforschung (C) 42:1328–1334
    [Google Scholar]
  9. Geleziunas R, Bour S, Boulerice F, FIiscott J, Wainberg M. 1991; Diminution of CD4 surface protein but not CD4 messenger RNA levels in monocytic cells infected by HIV-1. AIDS 5:29–33
    [Google Scholar]
  10. Gielen V, Schmitt D, Dezutter-Dambuyant C, Thivolbt J. 1989; AIDS and Langerhans cells: CD4 antigenic site density modification evidenced by single-cell immunogold labeling. Regional Immunology 2:7–13
    [Google Scholar]
  11. Hausmann EH. S, Gelderblom HR, Clapham PR, Pauli G, Weiss RA. 1987; Detection of HIV envelope specific antibodies by immunoelectron microscopy and correlation with antibody titre and virus neutralizing activity. Journal of Virological Methods 16:125–137
    [Google Scholar]
  12. Hecht T, Summers D. 1976; Interactions of vesicular stomatitis virus with murine cell surface antigens. Journal of Virology 19:833–845
    [Google Scholar]
  13. Henderson LE, Sowder R, Copeland TD, Oroszlan S, Arthur LO, Robey WG, Fischinger PJ. 1987; Direct identification of class II histocompatibility DR proteins in preparations of human T-cell lymphotropic virus type III. Journal of Virology 61:629–632
    [Google Scholar]
  14. Hildreth JEK, Orentas RJ. 1989; Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation. Science 244:1075–1078
    [Google Scholar]
  15. Holland JJ, Kiehn ED. 1970; Influenza virus effects on cell membrane proteins. Science 167:202–205
    [Google Scholar]
  16. Homsy J, Meyer M, Tateno M, Clarkson S, Levy JA. 1989; The Fc and not CD4 receptor mediates antibody enhancement of HIV infection in human cells. Science 244:1357–1360
    [Google Scholar]
  17. Hoxie JA, Alpers JD, Rackowski JL, Huebner K, Hag-garty BS, Cedarbaum AJ, Reed JC. 1986; Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV. Science 234:1123–1127
    [Google Scholar]
  18. June RA, Schade SZ, Bankowski MJ, Kuhns M, McNamara A, Lint TF, Landay AL, Spear GT. 1991; Complement and antibody mediate enhancement of HIV infection by increasing virus binding and provirus formation. AIDS 5:269–274
    [Google Scholar]
  19. Kerkau T, Schmitt-Landgraf R, Schimpl A, Wecker E. 1989; Downregulation of HLA class I antigens in HIV-1 infected cells. AIDS Research and Human Retroviruses 5:613–620
    [Google Scholar]
  20. Marx PA, Munn RJ, Joy KI. 1988; Methods in laboratory investigation. Computer emulation of thin section electron microscopy predicts an envelope-associated icosadeltahedral capsid for human immunodeficiency virus. Laboratory Investigation 58:112–118
    [Google Scholar]
  21. Meerloo T, Parmentier HK, Osterhaus ADME, Goudsmit J, Schuurman HJ. 1992; Modulation of cell surface molecules during HIV-1 infection of H9 cells. An immunoelectron microscopic study. AIDS 6: in press
    [Google Scholar]
  22. Meltzer MS, Stillman DR, Hoover DL, Hanson BD, Turpin JA, Kalter DC, Gendelman HE. 1990; HIV and the immune system. Immunology Today 11:217–223
    [Google Scholar]
  23. Metzelaar MJ, Schuurman HJ, Heijnen HFG, Sixma JJ, Nieuwenhuis HK. 1991; Biochemical and immunohistochemical characteristics of CD62 and CD63 monoclonal antibodies. Expression of GMP-140 and LIMP-CD63 CD63 antigen in human lymphoid tissue. Virchows Archives B 61:269–277
    [Google Scholar]
  24. Palmer E, Goldsmith CS. 1988; Ultrastructure of human retroviruses. Journal of Electron Microscopic Techniques 8:3–15
    [Google Scholar]
  25. Pantaleo G, Poli G, Butini L, Fox C, Dayton AI, Fauci AS. 1991; Dissociation between syncytia formation and HIV spreading. Suppression of syncytia formation does not necessarily reflect inhibition of HIV infection. European Journal of Immunology 21:1771–1774
    [Google Scholar]
  26. Schols D, Pauwels R, Desmyter J, De Clercq E. 1992; Presence of class II histocompatibility DR proteins on the envelope of human immunodeficiency virus demonstrated by FACS analysis. Virology 189:374–376
    [Google Scholar]
  27. Simons K, Garoff M. 1980; The budding mechanism of enveloped animal viruses. Journal of General Virology 50:1–21
    [Google Scholar]
  28. Stam NJ, Spits H, Ploegh HL. 1986; Monoclonal antibodies raised against denatured HLA-B locus heavy chains permit biochemical characterization of certain HLA-C locus products. Journal of Immunology 137:2299–2306
    [Google Scholar]
  29. Stevenson M, Zhang X, Volsky DJ. 1987; Downregulation of cell surface molecules during noncytopathic infection of T cells with human immunodeficiency virus. Journal of Virology 61:3741–3748
    [Google Scholar]
  30. Strauss EG. 1978; Mutants of Sindbis virus III. Host polypeptides present in purified HR and ts!03 virus particle. Journal of Virology 28:466–474
    [Google Scholar]
  31. Takeda A, Tuazon CU, Ennis FA. 1988; Antibody-enhanced infection by HIV-1 via Fc receptor-mediated entry. Science 242:580–583
    [Google Scholar]
  32. Tersmette M, Miedema F. 1990; Interactions between HIV and the host immune system in the pathogenesis of AIDS. AIDS 4 (Supplement 1):S57–S56
    [Google Scholar]
  33. Timâr J, Nagy K, Lapis K. 1986; Morphologic and immunoelectron microscopic identification of human T-cell lymphotropic virus type III (HTLV-III). Histology and Histopathology 1:43–47
    [Google Scholar]
  34. Valentin A, Lundin K, Patarroyo M, Âsjô B. 1990; The leukocyte adhesion glycoprotein CD 18 participates in HIV-1-induced syncytia formation in monocytoid and T cells. Journal of Immunology 144:934–937
    [Google Scholar]
  35. Weeks BS, Klotman ME, Dhawan S, Kibbey M, Rappaport J, Kleinman HK, Yamada KM, Klotman PE. 1991; HIV-1 infection of human T lymphocytes results in enhanced a α5β1 integrin expression. Journal of Cell Biology 144:847–853
    [Google Scholar]
  36. Young J, Bates P, Willert K, Varmus H. 1990; Efficient incorporation of human CD4 protein into avian leukosis virus particles. Science 250:1421–1423
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-1-129
Loading
/content/journal/jgv/10.1099/0022-1317-74-1-129
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error