1887

Abstract

An open reading frame (ORF 1) located upstream of the polyhedron envelope protein gene in the multinucleocapsid nuclear polyhedrosis virus (OpMNPV) genome was cloned in-frame into a bacterial expression vector. The fusion protein produced by this construct was used for the preparation of a monospecific antiserum. Western blot analysis of extracts from OpMNPV-infected cells and NPV (AcMNPV)-infected cells detected a 24K protein late in infection. This antiserum also reacted with a 24K protein in preparations of budded and polyhedra-derived virus from OpMNPV and AcMNPV. The 24K protein was not -glycosylated. Immunoelectron microscopy confirmed that the OpMNPV p24 is associated with nucleocapsids of budded and polyhedra-derived virions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-1-103
1993-01-01
2019-11-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-1-103
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error