RT Journal Article SR Electronic(1) A1 Kohara, Michinori A1 Tsukiyama-Kohara, Kyoko A1 Maki, Noboru A1 Asano, Kaori A1 Yamaguchi, Kenjiro A1 Miki, Keizaburo A1 Tanaka, Satoshi A1 Hattori, Nobu A1 Matsuura, Yoshiharu A1 Saito, Izumu A1 Miyamura, Tatsuo A1 Nomoto, AkioYR 1992 T1 Expression and characterization of glycoprotein gp35 of hepatitis C virus using recombinant vaccinia virus JF Journal of General Virology, VO 73 IS 9 SP 2313 OP 2318 DO https://doi.org/10.1099/0022-1317-73-9-2313 PB Microbiology Society, SN 1465-2099, AB Complementary DNA clones corresponding to one of the putative structural regions of the hepatitis C virus (HCV) genome were obtained from sera of non-A non-B hepatitis patients. The putative envelope gene was expressed by using a recombinant vaccinia virus carrying this region of the HCV genome. In cells infected with the recombinant vaccinia virus, a glycosylated protein with an M r of about 35K (gp35) was specifically detected by convalescent sera from hepatitis C patients. The sera from rabbits immunized with this recombinant vaccinia virus reacted to the gp35 produced in insect cells and also to gp35 which was translated in vitro in the glycosylated and processed form. The gp35 was used to detect antibodies in sera of only 7 to 23% of HCV patients at various stages of HCV disease. These results suggest that the gp35 of HCV may not have high antigenicity in humans., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-9-2313