1887

Abstract

Complementary DNA clones corresponding to one of the putative structural regions of the hepatitis C virus (HCV) genome were obtained from sera of non-A non-B hepatitis patients. The putative envelope gene was expressed by using a recombinant vaccinia virus carrying this region of the HCV genome. In cells infected with the recombinant vaccinia virus, a glycosylated protein with an of about 35K (gp35) was specifically detected by convalescent sera from hepatitis C patients. The sera from rabbits immunized with this recombinant vaccinia virus reacted to the gp35 produced in insect cells and also to gp35 which was translated in the glycosylated and processed form. The gp35 was used to detect antibodies in sera of only 7 to 23% of HCV patients at various stages of HCV disease. These results suggest that the gp35 of HCV may not have high antigenicity in humans.

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1992-09-01
2024-11-11
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References

  1. Chiba J., Ohba H., Matsuura Y., Watanabe Y., Katayama T., Kikuchi S., Sato I., Miyamura T. 1991; Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus. Proceedings of the National Academy of Sciences, U.S.A. 88:4641–4645
    [Google Scholar]
  2. Choo Q.-L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  3. Choo Q.-L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P. J., Weiner A. J., Bradley D. W., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A. 88:2451–2455
    [Google Scholar]
  4. Chu G., Hayakawa H., Berg P. 1987; Electroporation for efficient transfection of mammalian cells with DNA. Nucleic Acids Research 15:1311–1325
    [Google Scholar]
  5. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Shimotohno K. 1991; Gene mapping of putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proceedings of the National Academy of Sciences, U.S.A. 88:5547–5551
    [Google Scholar]
  6. Kaminski A., Howell M. T., Jackson R. J. 1990; Initiation of encephalomyocarditis virus RNA translation: the authentic initiation site is not selected by a scanning mechanism. EMBO Journal 9:3753–3759
    [Google Scholar]
  7. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A non-B hepatitis. Proceedings of the National Academy of Sciences, U.S.A. 87:9524–9528
    [Google Scholar]
  8. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Lefkowitch J. H., Apfelbaum T. F. 1987; Liver cell dysplasia and hepatocellular carcinoma in non-A, non-B hepatitis. Archives of Pathology and Laboratory Medicine 111:170–173
    [Google Scholar]
  11. Matsuura Y., Harada S., Suzuki R., Watanabe Y., Inoue Y., Saito I., Miyamura T. 1992; Expression of processed envelope protein of hepatitis C virus in mammalian and insect cells. Journal of Virology 66:1425–1431
    [Google Scholar]
  12. Muchmore E., Popper H., Peterson D. A., Miller M. F., Liberman H. M. 1988; Non-A, non-B hepatitis-related hepatocellular carcinoma in a chimpanzee. Journal of Medical Primatology 17:235–246
    [Google Scholar]
  13. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. 1990; The 5′-terminaI sequence of the hepatitis C virus genome. Japanese Journal of Experimental Medicine 60:167–177
    [Google Scholar]
  14. Saito M., Hasegawa A., Kashiwakuma T., Kohara M., Sugi M., Miki K., Yamamoto T., Mori H., Ohta Y., Tanaka E., Kiyosawa K., Furuta S., Tanaka S., Hattori N. 1992; Performance of ELISA system for anti-HCV using two new antigens (C11/C7). Clinical Chemistry (in press)
    [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  16. Shimizu K. Y., Iwamoto I., Hijikata M., Purcell R. H., Yoshikura H. 1992; Evidence for in vitro replication of hepatitis C virus genome in a human T cell line. Proceedings of the National Academy of Sciences, U.S.A. 89:5477–5481
    [Google Scholar]
  17. Takamizawa A., Mori C., Fuke I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
  18. Takeuchi K., Boonmar R., Kubo Y., Katayama T., Ohbayashi A., Choo Q.-L., Kuo G., Houghton M., Saito I., Miyamura T. 1990a; Hepatitis C virus cDNA clones isolated from a healthy carrier donor implicated in post transfusion non-A, non-B hepatitis. Gene 91:287–291
    [Google Scholar]
  19. Tsukiyama K., Yoshikawa Y., Kamata H., Imaoka K., Asano K., Funahashi S., Miyamura T., Shida H., Sugimoto M., Yamanouchi K. 1989; Development of heat-stable recombinant rinderpest vaccine. Archives of Virology 107:225–235
    [Google Scholar]
  20. Tsukiyama-Kohara K., Kohara M., Yamaguchi K., Maki N., Toyoshima A., Miki K., Tanaka S., Hattori N., Nomoto A. 1991; A second group of hepatitis C virus. Virus Genes 5:243–254
    [Google Scholar]
  21. Tsukiyama-Kohara K., Iizuka N., Kohara M., Nomoto A. 1992; Internal ribosome entry site within hepatitis C virus RNA. Journal of Virology 66:1476–1483
    [Google Scholar]
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