1887

Abstract

Poly(I).poly(CU) or interferon treatment inhibited multiplication of the xenotropic baboon type C endogenous retrovirus M7 in chronically infected human AV3-M7 cells, as determined by a reverse transcriptase (RT) assay and electron microscopy. Furthermore, this polynucleotide induced 2′5′ oligoadenylate (2′5′A) synthetase activity. In contrast to interferon (IFN), poly(I).poly(CU) did not give rise to the appearance of a trapping phenomenon observable by electron microscopy. When AV3-M7 cells were treated simultaneously with poly(I).poly(CU) and anti-IFN-β/α antibodies, the induction of 2′5′A synthetase was abolished without any alteration of the inhibitory effect of RT activity. Taken together, these results suggest that different mechanisms are used by poly(I).poly(CU) and IFN in blocking type C retrovirus multiplication.

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1992-09-01
2024-04-25
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References

  1. Absher M., Stinebring W. R. 1969; Toxic properties of a synthetic double-stranded RNA. Endotoxin-like properties of poly(I).poly(C), an interferon stimulator. Nature, London 223:715–717
    [Google Scholar]
  2. Adamson K. H. 1969; Toxic properties of a synthetic doublestranded RNA. Embryotoxic effect of poly(I).poly(C). Nature, London 223:718
    [Google Scholar]
  3. Aurelian L., Rinehart C. L., Wachsman M., Kulka M., Ts’o P.O.P. 1987; Augmentation of natural immune defence mechanisms and therapeutic potential of a mismatched double-stranded polynucleotide in cutaneous herpes simplex virus type 2 infection. Journal of General Virology 68:2831–2838
    [Google Scholar]
  4. Baglioni C., Nilsen T. W. 1983; Mechanisms of antiviral action of interferon. In Interferon vol 5 pp 23–42 Edited by Gresser I. London & New York: Academic Press;
    [Google Scholar]
  5. Ball L. A. 1982; 2′5′oligoadenylate synthetase. In The Enzymes, vol XV: Nucleic Acids, part B pp 281–313 Edited by Boyer P. D. New York: Academic Press;
    [Google Scholar]
  6. Billiau A., Heremous H., Allen P. T., De Maeyer-Guignard J., De Somer P. 1976; Trapping of oncornavirus particles at the surface of interferon treated cells. Virology 73:537–542
    [Google Scholar]
  7. Canivet M., Jouanny C., Fourcade A., Lasneret J., Rhodes-Feuillette A., Peries J. 1983; Effect of human interferon on type D retrovirus multiplication in chronically infected cell lines. Journal of Interferon Research 3:53–64
    [Google Scholar]
  8. Carter W. A., De Clercq E. 1974; Viral infection and host defense. Many aspects of viral infection and recovery can be explained by the modulatory role of double-stranded RNA. Science 186:1172–1178
    [Google Scholar]
  9. Carter W. A., Pitha P. M., Marshall L. W., Tazawa I., Tazawa S., Ts’o P. O. P. 1972; Structural requirement of the rIn.rCn complex for induction of human interferon. Journal of Molecular Biology 70:567–587
    [Google Scholar]
  10. Carter W. A., O’Malley J., Beeson M., Cunnington P., Kelvin A., Vere-Hodge A., Alderfer J. L., Ts’o P. O. P. 1976; An integrated and comparative study of the antiviral effect and other biological properties of the polyinosinic.polycytidylic acid duplex and its mismatched analogues. III Chronic effects and immunological features. Molecular Pharmacology 12:440–453
    [Google Scholar]
  11. Carter W. A., Strayer W. A., Strayer D. R., Brodsky I., Lewin M., Pellegrino M. G., Einck L., Henriques H. F., Simon G. L., Parenti D. M., Scheib R. G., Schulof R. S., Montefiori D. C., Robinson W. E., Mitchell W. M., Volsky D. J., Paul D., Paxton H., Meyer W. A., Kariko K., Reichenbach N., Suhadolnik R. J., Gillespie D. H. 1987; Clinical, immunological and virological effects of ampligen, a mismatched doublestranded RNA, in patients with AIDS-related complex. Lancet i:1286–1292
    [Google Scholar]
  12. Chapekar M. S., Glazer R. I. 1983; Effect of fibroblast and recombinant leukocyte interferons and double stranded RNA on ppp(2′-5′)An synthesis and cell proliferation in human colon carcinoma cells in vitro . Cancer Research 43:2683–2687
    [Google Scholar]
  13. Chapekar M. S., Glazer R. I. 1985; Synergistic effect of human immune interferon and double-stranded RNA against human colon carcinoma cells in vitro . Cancer Research 45:2539–2544
    [Google Scholar]
  14. Chapekar M. S., Glazer R. I. 1986; Potentiation of the cytocidal effect of human immune interferon by different synthetic doublestranded RNAs in the refractory human colon carcinoma cell line BE. Cancer Research 46:1698–1702
    [Google Scholar]
  15. Chapekar M. S., Knode M. C., Glazer R. I. 1988; The epidermal growth factor and interferon independent effects of double-stranded RNA in A431 cells. Molecular Pharmacology 34:461–466
    [Google Scholar]
  16. Chelbi-Alix M. K., Boissard C., Sripati C. E., Rosselin G., Thang M. N. 1991; VIP induces in HT29 cells 2′5′ oligoadenylate synthetase and antiviral state via interferon β/α synthesis. Peptides 12:1085–1093
    [Google Scholar]
  17. Clemens M. J., McNurlan M. A. 1985; Regulation of cell proliferation and differentiation by interferons. Biochemical Journal 226:345–360
    [Google Scholar]
  18. Coffin J. M. 1990; Retroviridae and their replication. In Virology pp 1437–1500 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  19. Haines D. S., Strauss K. I., Gillespie D. H. 1991; Cellular response to double-stranded RNA. Journal of Cellular Biochemistry 46:2–20
    [Google Scholar]
  20. Hearl W. G., Johnston M. I. 1986; A misaligned doublestranded RNA, poly(I).poly(C12U), induces accumulation of 2′5′-oligoadenylates in mouse tissue. Biochemical and Biophysical Research Communications 138:40–46
    [Google Scholar]
  21. Hovanessian A. G. 1991; Interferon-induced and double-stranded RNA-activated enzymes. A specific protein kinase and 2′5′ oligoadenylate synthetase. Journal of Interferon Research 11:199–206
    [Google Scholar]
  22. Hubbell H. R., Liu R. S., Maxwell B. L. 1984; Independent sensitivity of human tumor cell lines to interferon and double-standed RNA. Cancer Research 44:3252–3257
    [Google Scholar]
  23. Hubbell H. R., Boyer J. E., Roane P., Burch R. M. 1991; Cyclic AMP mediates the direct antiproliferative action of mismatched double-stranded RNA. Proceedings of the National Academy of Sciences, U.S.A. 88:906–910
    [Google Scholar]
  24. Johnston M. I., Torrence P. F. 1984 In Interferon 3. Mechanisms of Production and Actions pp 189–235 Edited by Friedman R. M. Amsterdam: Elsevier;
    [Google Scholar]
  25. Justesen J., Ferbus D., Thang M. N. 1980; 2′5′ oligoadenylate synthetase, an interferon-induced enzyme: direct assay methods for the products 2′5′ oligoadenylates and cooligonucleotides. Nucleic Acids Research 8:3073–3085
    [Google Scholar]
  26. Laurence J., Kulkosky J., Friedman S. M., Posnett D. N., Ts’o P. O. P. 1987; Poly I. polyC12U-mediated inhibition of loss of alloantigen responsiveness and viral replication in human CD4 + T cell clones exposed to human immunodeficiency virus in vitro . Journal of Clinical Investigation 80:1631–1639
    [Google Scholar]
  27. Lengyel P. 1982; Biochemistry of interferons and their actions. Annual Review of Biochemistry 51:251–282
    [Google Scholar]
  28. Lin S. L., Greene J. J., Ts’o P. O. P. 1982; Sensitivity and resistance of human tumour cells to interferon and rI.,.rC.,. Nature, London 297:417–419
    [Google Scholar]
  29. Lindsay H. L., Trown P. W., Brandt J., Forbes M. 1962; Toxic properties of a synthetic double-stranded RNA. Pyrogenicity of poly(I). poly(C) in rabbits. Nature, London 223:717–718
    [Google Scholar]
  30. Mémet S., Besancon F., Bourgeade M. F., Thang M. N. 1991; Direct induction of interferon γ and interferon α/β-inducible gene by double-stranded RNA. Journal of Interferon Research 11:131–141
    [Google Scholar]
  31. Mitchell W. M., Montefiori D. C., Robinson W. E. Jr 1987; Mismatched double-stranded RNA (Ampligen) reduces concentration of zidovudine (Azidothymidine) required for in vitro inhibition of human immunodeficiency virus. Lancet i:890–892
    [Google Scholar]
  32. Montefiori D. C., Mitchell W. M. 1987; Antiviral activity of mismatched double-stranded RNA against human immunodeficiency virus in vitro . Proceedings of the National Academy of Sciences, U.S.A. 84:2985–2989
    [Google Scholar]
  33. Montefiori D. C., Pellligrini M. G., Robinson E., Engle K., Feild M., Mitchell W. M., Gillespie D. H. 1989a; Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA. Biochemical and Biophysical Research Communications 158:943–950
    [Google Scholar]
  34. Montefiori D. C., Robinson W. E., Mitchell W. M. 1989b; In vitro evaluation of mismatched double-stranded RNA (ampligen) for combination therapy in the treatment of acquired immunodeficiency syndrome. Aids Research and Human Retroviruses 5:193–203
    [Google Scholar]
  35. O’Malley J. A., Leong S. S., Horoszewicz J. S., Carter W. A., Alderfer J. L., Ts’o P. O. P. 1979; Polyinosinic acid-polycytidylic acid and its mismatched analogues: differential effects on human cell function. Molecular Pharmacology 15:165–173
    [Google Scholar]
  36. Périés J., Canivet M., Rhodes-Feuillette A., Todaro G. J. 1979; Effect of interferon on chronic infection of human cells by xenotropic type C viruses. International Journal of Cancer 23:798–802
    [Google Scholar]
  37. Pestka S., Langer J. A., Zoon K. C., Samuel C. E. 1987; Interferons and their actions. Annual Review of Biochemistry 56:727–777
    [Google Scholar]
  38. Pine R., Decker T., Kessler D. S., Levy D. E., Darnell J. E. 1990; Purification and cloning of interferon-stimulated gene factor 2 (ISGF2): ISGF2 (ISGF-1) can bind to the promoters of both beta interferon- and interferon stimulated genes but is not a primary transcriptional activator of either. Molecular and Cellular Biology 10:2448–2457
    [Google Scholar]
  39. Randerath E., Randerath K. 1967; Ion-exchange thin layer chromatography. Chromatography 3:485–499
    [Google Scholar]
  40. Saal F., Roseto A., Tobaly J., Peries J. 1981; Etude de la susceptibilité de cellules humaines cultivées in vitro à l’infection par des rétrovirus xénotropiques de type C. Comptes Rendus de l’Academie des Sciences 292: serie III 1203–1206
    [Google Scholar]
  41. Spector T. 1978; Refinement of the Coomassie blue method of protein quantitation. Analytical Biochemistry 86:142–146
    [Google Scholar]
  42. Stewart W. E. III 1979 The Interferon System New York: Springer-Verlag;
    [Google Scholar]
  43. Thang M. N., Guschlbauer W. 1992; Actions biologiques et perspectives therapeutiques des polyribonucleotides double-brins: une réévaluation. Pathologie-Biologie (in press)
    [Google Scholar]
  44. Torrence P. F., Imai J., Lesiak K., Johnston M. I., Jacobsen H., Friedman R. M., Sawai H., Safer B. 1982; Double-stranded RNA and 2′5′ oligoadenylates: companions in interferon action?. In Interferons pp 123–142 Edited by Merigan T. C., Friedman R. M. New York: Academic Press;
    [Google Scholar]
  45. Ts’o P. O. P., Alderfer J. L., Levy J., Marshall L. W., O’Malley J., Horoszewicz J. S., Carter W. A. 1976; An integrated and comparative study of the antiviral effects and other biological properties of the polyinosinic acid-polycytidylic acid and its mismatched analogues. Molecular Pharmacology 12:299–312
    [Google Scholar]
  46. Whathelet M. G., Clauss I. M., Nols C. B., Content J., Huez G. A. 1987; New inducers revealed by the promoter sequence analysis of two interferon-activated human genes. European Journal of Biochemistry 169:313–321
    [Google Scholar]
  47. Whathelet M. G., Clauss I. M., Content J., Huez G. A. 1988; Regulation of two interferon-inducible human genes by interferon, poly (rl). poly(rC) and viruses. European Journal of Biochemistry 174:323–329
    [Google Scholar]
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