1887

Abstract

The regions of the fusion protein of respiratory syncytial virus (RSV) that react with neutralizing, fusion-inhibiting and highly protective bovine and murine monoclonal antibodies (MAbs) were mapped by two methods: (i) competitive binding assays and (ii) production and analysis of antibody-escape mutants. Competitive binding assays with 16 murine and 10 bovine MAbs identified 11 antigenic sites on the fusion (F) protein, many of which overlapped extensively, and indicated that cattle, a natural host for RSV, and mice recognize similar epitopes. Neutralizing MAbs identified four sites, two of which were also fusion-inhibiting and highly protective in mice. The pattern of reactivity of antibody-escape mutants with the MAbs confirmed the mapping of the protective epitopes deduced from competitive binding assays. A comparison of the biological properties of MAbs to the F protein indicated that protection against RSV infection correlated with fusion inhibition rather than neutralization titre or complement-dependent lysis of virus-infected cells.

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1992-09-01
2021-10-22
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References

  1. Arbiza J., Taylor G., Lopéz J. A., Furze J., Wyld S., Whyte P., Stott E. J., Wertz G., Sullender W., Trudel M., Melero J. A. 1992; Characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus. Journal of General Virology 73:2225–2234
    [Google Scholar]
  2. Beeler J. A., Van Wyke Coelingh K. 1989; Neutralization epitopes of the F glycoprotein of respiratory syncytial virus: effect of mutation upon fusion function. Journal of Virology 63:2941–2950
    [Google Scholar]
  3. Belshe R. B., Van Voris L. P., Mufson M. A. 1982; Parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. Journal of Infectious Diseases 145:311–319
    [Google Scholar]
  4. Bourgeois C., Corvaisier C., Bour J. B., Kohli E., Pothier P. 1991; Use of synthetic peptides to locate neutralizing antigenic domains on the fusion protein of respiratory syncytial virus. Journal of General Virology 72:1051–1058
    [Google Scholar]
  5. Collins P. L., Huang Y. T., Wertz G. W. 1984; Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A. 81:7683–7687
    [Google Scholar]
  6. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. 1985; Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of FI subunit. Nucleic Acids Research 13:1559–1573
    [Google Scholar]
  7. Fernie B. F., Ford E. C., Gerin J. I. 1981; The development of BALB/c cells persistently infected with respiratory syncytial virus: presence of ribonucleoprotein on the cell surface. Proceedings of the Society for Experimental Biology and Medicine 167:83–86
    [Google Scholar]
  8. Fulginiti V. A., Eller J. J., Sieber O. F., Joyner J. W., Minamitani M., Meiklejohn G. 1969; Respiratory virus vaccines; an aqueous trivalent parainfluenza virus vaccine and an alum precipitated respiratory syncytial virus vaccine. American Journal of Epidemiology 89:435–448
    [Google Scholar]
  9. Garcia-Barreno B., Palomo C., Penas C., Delgardo T., Perez-Brena P., Melero J. A. 1989; Marked differences in the antigenic structure of human respiratory syncytial virus F and G glycoproteins. Journal of Virology 63:925–932
    [Google Scholar]
  10. Gilks G. R. 1990 Leukocyte Typing Database IV Oxford: Oxford University Press;
    [Google Scholar]
  11. Kennedy H. E., Jones B. V., Tucker E. M., Ford N. J., Clarke S. W., Furze J., Thomas L. H., Stott E. J. 1988; Production and characterization of bovine monoclonal antibodies to respiratory syncytial virus. Journal of General Virology 69:3023–3032
    [Google Scholar]
  12. Kim H. W., Arrobio J. G., Brandt C. D., Jeffries B. C., Pyle G., Reid J. L., Chanock R. M., Parrott R. H. 1973; Epidemiology of respiratory syncytial infection in Washington, D. C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. American Journal of Epidemiology 98:216–225
    [Google Scholar]
  13. Kimman T. G., Sol J., Westenbrink F., Straver P. J. 1989; A severe outbreak of respiratory tract disease associated with bovine respiratory syncytial virus probably enhanced by vaccination with modified live virus. Veterinary Quarterly 11:250–253
    [Google Scholar]
  14. Lerch R. A., Stott E. J., Wertz G. W. 1989; Characterization of bovine respiratory syncytial virus proteins and mRNAs and generation of cDNA clones to the viral mRNAs. Journal of Virology 63:833–840
    [Google Scholar]
  15. Lerch R. A., Anderson K., Wertz G. W. 1990; Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. Journal of Virology 64:5559–5569
    [Google Scholar]
  16. Lewis F. A., Rae M. L., Lehman N. I., Ferris A. A. 1961; A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Medical Journal of Australia 48:932–933
    [Google Scholar]
  17. Lopez J. A., Penas C., Garcia-Barreno B., Melero J. A., Portela A. M. 1990; Location of a highly conserved neutralizing epitope in the F glycoprotein of human respiratory syncytial virus. Journal of Virology 64:927–930
    [Google Scholar]
  18. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Collins P. 1986; Expression of the F1 glycoprotein of respiratory syncytial virus: comparison of the individual contributions of the F and G glycoproteins to host immunity. Proceedings of the National Academy of Sciences, U.S.A. 83:7462–7466
    [Google Scholar]
  19. Paradiso P. R., Hu B. T., Armugham R., Hildreth S. 1991 Mapping of a fusion related epitope of the respiratory syncytial virus fusion protein, Vaccine 9:231–237
    [Google Scholar]
  20. Scopes G. E., Watt P. J., Lambden P. R. 1990; Identification of a linear epitope on the fusion glycoprotein of respiratory syncytial virus. Journal of General Virology 71:53–59
    [Google Scholar]
  21. Stott E. J., Thomas L. H., Collins A. P., Crouch S., Jebbett J., Smith G. S., Luther P. D., Caswell R. 1980; A survey of virus infections of the respiratory tract of cattle and their association with disease. Journal of Hygiene 85:257–270
    [Google Scholar]
  22. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K.-Y., King A. M. Q., Wertz G. W. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology 61:3855–3861
    [Google Scholar]
  23. Taketa K., Ichikawa E., Taga H., Hirai H. 1985; Antibody-affinity blotting, a sensitive technique for the detection of α- foetoprotein separated by lectin affinity electrophoresis in agarose gels. Electrophoresis 6:492–497
    [Google Scholar]
  24. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbett J. 1984a; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  25. Taylor G., Stott E. J., Hughes M., Collins A. P. 1984b; Respiratory syncytial virus infection in mice. Infection and Immunity 43:649–655
    [Google Scholar]
  26. Tempest P. R., Bremner P., Lambert M., Taylor G., Furze J. M., Carr F. J., Harris W. J. 1991; Reshaping a human monoclonal antibody to inhibit human respiratory syncytial virus infection in vivo . Bio I Technology 9:266–271
    [Google Scholar]
  27. Trudel M., Nadon F., Seguin C., Payment P., Talbot P. T. 1987a; Respiratory syncytial virus fusion glycoprotein: further characterization of a major epitope involved in virus neutralisation. Canadian Journal of Microbiology 33:933–938
    [Google Scholar]
  28. Trudel M., Nadon F., Seguin C., Dionne G., Lacroix M. 1987b; Identification of a synthetic peptide as part of a major neutralization epitope of respiratory syncytial virus. Journal of General Virology 68:2273–2280
    [Google Scholar]
  29. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984; Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infection and Immunity 43:756–758
    [Google Scholar]
  30. Walsh E. E., Cote P. J., Fernie B. F., Schlesinger J. J., Brandriss M. W. 1986; Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies. Journal of General Virology 67:505–513
    [Google Scholar]
  31. Zygraich N. 1982; Vaccination against RS virus: a five year experience with Rispoval. In Proceedings of the 12th World Congress on Diseases of Cattle Amsterdam; The Netherlands: vol 1 pp 189–195
    [Google Scholar]
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