1887

Abstract

The nucleotide sequence of raspberry ringspot nepovirus (RRV) RNA-2 consists of 3928 nucleotides and a poly(A) tract at the 3′ end. RNA-2 contains one open reading frame which encodes a polypeptide of 123508 (123K). Edman degradation located the N terminus of the coat protein 514 residues from the C-terminal end of the 123K protein, which suggests that the coat protein is released from the polyprotein by cleavage of a C-A bond. The RRV coat protein has some sequence similarities with the coat proteins of other nepoviruses, but is no more like any one nepovirus than another. In contrast, the portion of the 123K protein to the N-terminal side of the coat protein is similar in sequence to the corresponding parts of the polyproteins of tomato black ring and grapevine chrome mosaic nepoviruses, though not to those of other nepoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-9-2189
1992-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/9/JV0730092189.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-9-2189&mimeType=html&fmt=ahah

References

  1. Acosta O., Mayo M. A. 1990a; Accumulation of different types of raspberry ringspot nepovirus particle in infected Nicotiana protoplasts. Journal of General Virology 71:713–717
    [Google Scholar]
  2. Acosta O., Mayo M. A. 1990b; Unusual electrophoretic properties of the coat protein of raspberry ringspot nepovirus. Intervirology 31:31–37
    [Google Scholar]
  3. Bertioli D. J., Harris R. D., Edwards M. L., Cooper J. I., Hawes W. S. 1991; Transgenic plants and insect cells expressing the coat protein of arabis mosaic virus produce empty virus-like particles. Journal of General Virology 72:1801–1809
    [Google Scholar]
  4. Brault V., Hibrand L., Candresse T., Le Gall O., Dunez J. 1989; Nucleotide sequence and genetic organization of Hungarian grapevine chrome mosaic nepovirus RNA 2. Nucleic Acids Research 17:7809–7819
    [Google Scholar]
  5. Demangeat G., Hemmer O., Fritsch C., Le Gall O., Candresse T. 1991; In vitro processing of the RNA-2-encoded polyprotein of two nepoviruses: tomato black ring virus and grapevine chrome mosaic virus. Journal of General Virology 72:247–252
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–143
    [Google Scholar]
  8. Francki R. I. B., Milne R. G., Hatta T. 1985; Nepovirus group. In Atlas of Plant Viruses Vol II pp 23–38 Raton: CRC Press;
    [Google Scholar]
  9. Fuchs M., Pinck M., Serghini M. A., Ravelonandro M., Walter B., Pinck L. 1989; The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain FI3. Journal of General Virology 70:955–962
    [Google Scholar]
  10. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  11. Harrison B. D. 1958; Further studies on raspberry ringspot and tomato black ring, soil-borne viruses that affect raspberry. Annals of Applied Biology 46:571–584
    [Google Scholar]
  12. Harrison B. D. 1964; Specific nematode vectors for serologically distinctive forms of raspberry ringspot and tomato black ring viruses. Virology 22:544–550
    [Google Scholar]
  13. Harrison B. D., Murant A. F., Mayo M. A. 1972; Evidence for two functional RNA species in raspberry ringspot virus. Journal of General Virology 16:339–348
    [Google Scholar]
  14. Harrison B. D., Murant A. F., Mayo M. A., Roberts I. M. 1974; Distribution of determinants for symptom production, host range and nematode transmissibility between the two RNA components of raspberry ringspot virus. Journal of General Virology 22:233–247
    [Google Scholar]
  15. Hewick R. M., Hunkerpiller M. W., Hood L. E., Dreyer W. J. 1981; A gas-liquid solid phase peptide and protein sequenator. Journal of Biological Chemistry 256:7990–7997
    [Google Scholar]
  16. Jones A. T., Mayo M. A., Henderson S. M. 1985; Biological and biochemical properties of an isolate of cherry rasp leaf virus from red raspberry. Annals of Applied Biology 106:101–110
    [Google Scholar]
  17. Mayo M. A., Murant A. F., Harrison B. D. 1971; New evidence on the structure of nepoviruses. Journal of General Virology 12:175–178
    [Google Scholar]
  18. Mayo M. A., Barker H., Harrison B. D. 1979; Polyadenylate in the RNA of five nepoviruses. Journal of General Virology 43:603–610
    [Google Scholar]
  19. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  20. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L. 1989; Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology 70:1037–1051
    [Google Scholar]
  21. Mayo M. A., Jolly C. A., Murant A. F., Raschke J. H. 1991; Nucleotide sequence of raspberry bushy dwarf virus RNA-3. Journal of General Virology 72:469–472
    [Google Scholar]
  22. Meyer M., Hemmer O., Mayo M. A., Fritsch C. 1986; The nucleotide sequence of tomato black ring virus RNA-2. Journal of General Virology 67:1257–1271
    [Google Scholar]
  23. Murant A. F. 1978; Raspberry ringspot virus. CMI/AAB Descriptions of Plant Viruses no 198
    [Google Scholar]
  24. Murant A. F. 1981; Nepoviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 197–238 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  25. Murant A. F., Taylor M., Duncan G. D., Raschke J. E. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  26. Palmenberg A. 1987; Genome organization, translation and processing in picomaviruses. In The Molecular Biology of the Positive Strand RNA Viruses pp 1–15 Edited by Rowlands D. J., Mayo M. A., Mahy B. W. J. London: Academic Press;
    [Google Scholar]
  27. Rott M. E., Tremaine J. H., Rochon D. M. 1991; Nucleotide sequence of tomato ringspot virus RNA-2. Journal of General Virology 72:1505–1514
    [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  30. Serghini M. A., Fuchs M., Pinck M., Reinbolt J., Walter B., Pinck L. 1990; RNA2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location. Journal of General Virology 71:1433–1441
    [Google Scholar]
  31. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  32. Staden R. 1984; Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Research 12:521–538
    [Google Scholar]
  33. Strauss J. H., Strauss E. 1990; Alphavirus proteinases. Seminars in Virology 1:347–356
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-9-2189
Loading
/content/journal/jgv/10.1099/0022-1317-73-9-2189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error