@article{mbs:/content/journal/jgv/10.1099/0022-1317-73-8-2121, author = "Oxelfelt, P. and Shanks, M. and Widmark, A. K. and Lomonossoff, G. P.", title = "Identification and characterization of pseudo-recombinants of red clover mottle comovirus", journal= "Journal of General Virology", year = "1992", volume = "73", number = "8", pages = "2121-2124", doi = "https://doi.org/10.1099/0022-1317-73-8-2121", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-8-2121", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "cDNA clones specific for the two genomic RNAs of strain O of the comovirus red clover mottle virus (RCMV) were constructed. Using these clones, in conjunction with clones specific for RNAs of RCMV strain S, local lesion isolates containing reciprocal pseudo-recombinants between strains S and O were identified. Investigation of the biological properties of these pseudo-recombinants showed that the ability of RCMV to infect Chenopodium quinoa is determined by B RNA. The results also suggest that both RNAs are involved in symptom formation in Pisum sativum. Analysis of the strain O clones enabled the sequences at the 3′ ends of both genomic RNAs of strain O to be determined. Comparison of these sequences with the corresponding region of the strain S RNAs suggests that the 3′ terminal sequences critical for replicase recognition may lie somewhat upstream of the poly(A) tract.", }