cDNA clones specific for the two genomic RNAs of strain O of the comovirus red clover mottle virus (RCMV) were constructed. Using these clones, in conjunction with clones specific for RNAs of RCMV strain S, local lesion isolates containing reciprocal pseudo-recombinants between strains S and O were identified. Investigation of the biological properties of these pseudo-recombinants showed that the ability of RCMV to infect is determined by B RNA. The results also suggest that both RNAs are involved in symptom formation in . Analysis of the strain O clones enabled the sequences at the 3′ ends of both genomic RNAs of strain O to be determined. Comparison of these sequences with the corresponding region of the strain S RNAs suggests that the 3′ terminal sequences critical for replicase recognition may lie somewhat upstream of the poly(A) tract.


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