1887

Abstract

A region of the fusion protein of measles virus (residues 240 to 252) was predicted to contain a B and a T epitope. A synthetic peptide representing this sequence was shown to induce both T and B cell reactivity in several inbred strains of mice, but the responses were clearly major histocompatibility complex-restricted. Elongation of this peptide by six residues at the C terminus on the basis of predictions for B cell epitopes resulted not only in increased peptide immunogenicity in some strains of mice but also produced strain-related positive and negative effects on the recognition of the peptide. BALB/c and SWR mice were non-responders to the short version of the peptide but responded well to the elongated form. On the other hand, the injection of the elongated peptide into C57BL/6 mice resulted in a loss of both B and T cell responsiveness seen with the short version. These results indicate the importance of flanking sequences on the immunogenicity and antigenicity of synthetic B and T cell epitopes and highlight the necessity to determine the most appropriate size of peptide to be used as an immunogen.

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1992-08-01
2022-12-08
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References

  1. Alkhatib G., Briedis D. J. 1986; The predicted primary structure of the measles virus hemagglutinin. Virology 150:479–484
    [Google Scholar]
  2. BerzoPsky J. A., Cease K. B., Cornette J. L., Spouge J. L., Margaret H., Berkower I. J., Good M. F., Miller L. H., de Lisi C. 1987; Protein antigenic structures recognized by T cells: potential applications to vaccine design. Immunological Reviews 98:9–30
    [Google Scholar]
  3. Bhayani H., Patterson Y. 1989; Analysis of peptide binding patterns in different major histocompatibility complex/T cell receptors complexes using pigeon cytochrome C-specific T cell hybridomas. Evidence that a single peptide binds major histocompatibility complex in different conformations. Journal of Experimental Medicine 170:1609–1625
    [Google Scholar]
  4. Brett S. J., Cease K. B., Berzofsky J. A. 1988; Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. Journal of Experimental Medicine 168:357–373
    [Google Scholar]
  5. Buus S., Sette A., Colon S. M., Miles G., Grey H. M. 1987; The relation between major histocompatibility complex (MHC) restriction and the capacity of la to bind immunogenic peptides. Science 235:1353–1358
    [Google Scholar]
  6. Choppin P. W., Richardson C. D., Merz D. C., Hall W. W., Scheid A. 1981; The functions and inhibition of the membrane glycoproteins of paramyxoviruses and myxoviruses and the role of the measles virus M protein in subacute sclerosing panencephalitis. Journal of Infectious Diseases 143:352–368
    [Google Scholar]
  7. Donermeyer D. L., Allen P. M. 1989; Binding to la protects an immunogenic peptide from proteolytic degradation. Journal of Immunology 142:1063–1068
    [Google Scholar]
  8. Drillien R., Spehner D., Kirn A., Giraudon P., Buckland R., Wild F., Lecocq J. P. 1988; Protection of mice from fatal measles encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein. Proceedings of the National Academy of Sciences, U.S.A 85:1252–1256
    [Google Scholar]
  9. Falk K., Rotzschke O., Stevanovic S., Jung G., Rammensee H. G. 1991; Allele-specific motifs revealed by sequencing of selfpeptides eluted from MHC molecules. Nature, London 351:290–296
    [Google Scholar]
  10. Francis M. J., Fry C. M., Rowlands D. J., Bittle J. L., Houghten R. A., Lerner R. A., Brown F. 1987; Immune response to uncoupled peptides of foot-and-mouth disease virus. Immunology 61:1–6
    [Google Scholar]
  11. Gammon G., Shastri N., Cogswell J., Wilbur S., Nasseri S., Krzych U., Miller A., Sercarz E. 1987; The choice of T-cell epitopes utilized on a protein antigen depends on multiple factors distant from, as well as at the determinant site. Immunological Reviews 98:53–73
    [Google Scholar]
  12. Jardetzky T. S., Lane W. S., Robinson R. A., Madden D. R., Wiley D. C. 1991; Identification of self peptides bound to purified HLA-B27. Nature, London 353:326–329
    [Google Scholar]
  13. Karplus J. A., Schultz G. E. 1985; Prediction of chain flexibility in proteins. A tool for the selection of peptide antigens. Naturwissenschaften 72:212–213
    [Google Scholar]
  14. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–143
    [Google Scholar]
  15. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  16. Lehmann P. V., Cardinaux F., Appella E., Muller S., Falcioni F., Adorini L., Nagy Z. A. 1989; Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking. European Journal of Immunology 19:1071–1077
    [Google Scholar]
  17. Livingstone A. M., Fathman C. G. 1987; The structure of T-cell epitopes. Annual Review of Immunology 5:477–501
    [Google Scholar]
  18. Malvoisin E., Wild F. 1990; Contribution of measles virus fusion protein in protective immunity: anti-F monoclonal antibodies neutralize virus infectivity and protect mice against challenge. Journal of Virology 64:5160–5162
    [Google Scholar]
  19. Margalit H., Spouge J. L., Cornett J. L., Cease K. B., de Lisi C., Berzofsky J. A. 1987; Prediction of immunodominant helper T cell antigenic sites from the primary sequence. Journal of Immunology 138:2213–2229
    [Google Scholar]
  20. Mertz D. C., Scheid A., Choppin P. W. 1980; Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. Journal of Experimental Medicine 151:275–288
    [Google Scholar]
  21. Milich D. R. 1989; Synthetic T and B cell recognition sites: implications for vaccine development. Advances in Immunology 45:195–264
    [Google Scholar]
  22. Norrby E., Enders-Ruckle G., ter Meulen V. 1975; Differences in the appearances of antibodies to structural components of measles virus after immunization with inactivated and live virus. Journal of Infectious Diseases 132:262–269
    [Google Scholar]
  23. Petty R. E., Steward M. W., Soothill J. F. 1972; The heterogeneity of antibody affinity in inbred mice and its possible immunopathologic significance. Clinical and Experimental Immunology 12:231–241
    [Google Scholar]
  24. Richardson C., Hull D., Greer P., Hasel K., Berkovich A., England G., Bellini W., Rima B., Lazzarini R. 1986; The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses. Virology 155:508–523
    [Google Scholar]
  25. Rothbard J., Taylor W. 1988; A sequence pattern common to T cell epitopes. EMBO Journal 7:93–98
    [Google Scholar]
  26. Rudensky A. Y., Hurlburt P. P., Hong S. C., Barlow A., Janeway C. A. 1991; Sequence analysis of peptides bound to MHC class II molecules. Nature, London 353:622–627
    [Google Scholar]
  27. Thornton J. M., Edwards M. S., Taylor W. R., Barlow D. J. 1986; Localization of continuous antigenic determinants in the protruding regions of proteins. EMBO Journal 5:409–413
    [Google Scholar]
  28. Vacchio M. S., Berzofsky J. A., Krzych U., Smith J. A., Hodes R. J., Finnegan A. 1989; Sequences outside a minimal immunodominant site exert negative effects on the recognition by staphylococcal nuclease-specific T cell clones. Journal of Immunology 143:2814–2819
    [Google Scholar]
  29. Wilmot C. M., Thornton J. M. 1988; Analysis and prediction of different types of β-turns in proteins. Journal of Molecular Biology 203221–232
    [Google Scholar]
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