1887

Abstract

Polypeptides from purified virions of the calf rotavirus (RV) isolate 993/83 and those from the pigeon RV isolate PO-13 comigrated on SDS-polyacrylamide gels. Two polypeptides of 45K and 47K were detected at the position of VP6. Both proteins behaved like authentic VP6 protein with EDTA and heat treatment. RV 993/83 and PO-13 showed identical one-dimensional peptide maps for VP2, and the 45K and 47K proteins. More than 70% of sera from German cattle older than 1 year showed neutralizing serum antibodies to RV 993/83 and RV PO-13.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-7-1873
1992-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/7/JV0730071873.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-7-1873&mimeType=html&fmt=ahah

References

  1. Brüssow H., Eichhorn W., Rohwedder A., Snodgrass D., Sidoti J. 1991a; Cattle develop neutralizing antibodies to rotavirus serotypes which could not be isolated from faeces of symptomatic calves. Journal of General Virology 72:1559–1567
    [Google Scholar]
  2. Brussow H., Offit P., Sidoti J. 1991b; Neutralizing antibodies to heterologous animal rotavirus serotypes 5, 6, 7, and 10 in sera from Ecuadorian children. Journal of Clinical Microbiology 29:869–873
    [Google Scholar]
  3. Brussow H., Nakagomi O., Gerna G., Eichhorn W. 1992; Isolation of an avianlike group A rotavirus from a calf with diarrhea. Journal of Clinical Microbiology 30:67–73
    [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. Cohen J., Laporte J., Charpilienne A., Scherrer R. 1979; Activation of RNA polymerase by calcium chelation. Archives of Virology 60:177–186
    [Google Scholar]
  6. Eichhorn W., Krauss M., Bachmann P. A., Mayr A. 1985; Vorkommen und Verbreitung atypischer Rotaviren bei Kalbem in Deutschland. Tierärtzliche Umschau 40:435–436
    [Google Scholar]
  7. Gorziglia M., Larrea C., Liprandi F., Esparza J. 1985; Biochemical evidence for the oligomeric (possibly trimeric) structure of the major inner capsid polypeptide (45K) of rotaviruses. Journal of General Virology 66:1889–1900
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. McCrae M. A., Faulkner-Valle G. P. 1981; Molecular biology of rotaviruses. I. Characterization of basic growth parameters and pattern of macromolecular synthesis. Journal of Virology 39:490–496
    [Google Scholar]
  10. McNulty M. S. 1978; Rotaviruses. Journal of General Virology 40:1–18
    [Google Scholar]
  11. Minamoto N., Oki K., Tomita M., Kinjo T., Suzuki Y. 1988; Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures. Epidemiology and Infection 100:481–492
    [Google Scholar]
  12. Sabara M., Ready K. F. M., Frenchick P. J., Babiuk L. A. 1987; Biochemical evidence for the oligomeric arrangement of bovine rotavirus nucleocapsid protein and its possible significance in the immunogenicity of this protein. Journal of General Virology 68:123–133
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-7-1873
Loading
/content/journal/jgv/10.1099/0022-1317-73-7-1873
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error