%0 Journal Article %A Martín, J. %A Albo, C. %A Ortín, J. %A Melero, J. A. %A Portela, A. %T In vitro reconstitution of active influenza virus ribonucleoprotein complexes using viral proteins purified from infected cells %D 1992 %J Journal of General Virology, %V 73 %N 7 %P 1855-1859 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-73-7-1855 %I Microbiology Society, %X A procedure to obtain RNA-free preparations containing the nucleoprotein (NP) and polymerase (P) proteins from influenza virus-infected cell extracts has been developed. The influenza virus P proteins present in these preparations copied small synthetic RNA molecules derived from plasmid sequences. In addition, RNA molecules encapsidated with the NP and P proteins were amplified and packaged into virus particles when transfected into influenza virus-infected cells. Thus, the preparations of NP and P proteins display features similar to those isolated from purified influenza virions, and represent an alternative for the preparation of active influenza virus P protein. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-7-1855