1887

Abstract

The regulatory and accessory proteins of human immunodeficiency virus type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We have used HIV-1-specific oligonucleotide primer pairs in a polymerase chain reaction procedure on RNA from lymphocyte cell lines infected with HIV-1. The amplified products were analysed by hybridization with splice junction-specific oligonucleotide probes to determine splice donor/splice acceptor combination utilization, subcloned into a plasmid vector and the nucleotide sequences were obtained. Two novel splice acceptor sites have been identified.

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/content/journal/jgv/10.1099/0022-1317-73-7-1825
1992-07-01
2019-11-21
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-7-1825
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