1887

Abstract

The human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev stimulates expression of structural viral proteins via a target response element (RRE) located within and mRNAs. To analyse the HIV-2 Rev trans-activation effect on the expression of the envelope protein, we cloned a functionally active HIV-2 cDNA and showed that it contained four exons. Using transient expression assays, we mapped a 353 bp RRE fragment within the gene of HIV-2 on which both HIV-1 and HIV-2 Rev could act. Interestingly, smaller fragments suppressed the use of additional splice sites within the gene and caused envelope protein expression independent of Rev.

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1992-07-01
2024-12-05
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References

  1. Ahmed Y. F., Gilmartin G. M., Hanly S. M., Nevins J. R., Greene W. C. 1991; The HTLV-I rex response element mediates a novel form of mRNA poly adenylation. Cell 64:727–737
    [Google Scholar]
  2. Benko D. M., Schwartz S., Pavlakis G. N., Felber B. K. 1990; A novel human immunodeficiency virus type 1 protein, tev, shares sequences with tat, env, and rev proteins. Journal of Virology 64:2505–2518
    [Google Scholar]
  3. Chang D. D., Sharp P. A. 1989; Regulation by HIV Rev depends upon recognition of splice sites. Cell 59:789–795
    [Google Scholar]
  4. Cullen B. R. 1987; Use of eukaryotic expression technology in the functional analysis of cloned genes. Methods in Enzymology 152:684–704
    [Google Scholar]
  5. Daly T. J., Cook K. S., Gray G. S., Maione T. E., Rusche J. R. 1989; Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro. Nature, London 342:816–819
    [Google Scholar]
  6. Dayton A. I., Sodroski J. G., Rosen C. A., Goh W. C., Haseltine W. A. 1986; The trans-activator gene of the human T-cell lymphotropic virus type III is required for replication. Cell 44:941–947
    [Google Scholar]
  7. Dayton E. T., Powell D. M., Dayton A. I. 1989; Functional analysis of CAR, the target sequence for the rev protein of HIV1. Science 246:1625–1629
    [Google Scholar]
  8. Dillon P. J., Nelbock P., Perkins A., Rosen C. 1990; Function of the human immunodeficiency viruses type 1 and 2 Rev proteins is dependent on their ability to interact with structured region present in env gene mRNA. Journal of Virology 64:4428–4437
    [Google Scholar]
  9. Emerman M., Guyader M., Montagnier L., Baltimore D., Muesing M. 1987; The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1. EMBO Journal 6:3755–3760
    [Google Scholar]
  10. Emerman M., Vazeux R., Peden K. 1989; The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization. Cell 57:1155–1165
    [Google Scholar]
  11. Favorolo J., Treisman R., Kamen R. 1980; Transcription maps of polyoma virus-specific RNA: analysis by two dimensional nuclease SI gel mapping. Methods in Enzymology 65:718–749
    [Google Scholar]
  12. Feinberg M. B., Jarrett R. F., Aldovini A., Gallo R. C., Wong-Staal F. 1986; HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA. Cell 46:807–817
    [Google Scholar]
  13. Felber B. K., Hadzopoulo-Cladaras M., Cladaras C., Copeland T., Pavlakis G. N. 1989; Rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA. Proceedings of the National Academy of Sciences, U.S.A. 86:1495–1499
    [Google Scholar]
  14. Fisher A. G., Feinberg M. B., Joseph S. F., Harper M. E., Marselle L. M., Reyes G., Gonda M. A., Aldovini A., Debouk C., Gallo R. C., Wong-Staal F. 1986; The transactivator gene of HTLV–III is essential for virus-replication. Nature, London 320:367–371
    [Google Scholar]
  15. Fourney R. M., Miyakoshi J., Day R. S., Paterson M. C. 1988; Northern blotting: efficient RNA staining and transfer. Focus 10:5–7
    [Google Scholar]
  16. Gluzman Y. 1981; SV40-transformed simian cells support the replication of early mutants. Cell 23:175–182
    [Google Scholar]
  17. Gorman C., Moffat L., Howard B. 1982; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  18. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  19. Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., Alizon M. 1987; Genome organization and transactivation of the human immunodeficiency virus type 2. Nature, London 326:662–669
    [Google Scholar]
  20. Heaphy S., Dingwall C., Ernberg I., Gait M. J., Green S. M., Karn J., Lowe A. D., Singh M., Skinner M. A. 1990; HIV1 regulator of virion expression (Rev) protein binds to an RNA stemloop structure located within the Rev response element region. Cell 60:685–693
    [Google Scholar]
  21. Kiems J., Frankel A. D., Sharp P. A. 1991; Specific regulation of mRNA splicing in vitro by a peptide from HIV-1 Rev. Cell 67:169–178
    [Google Scholar]
  22. Knight D. M., Flomerfelt F. A., Ghrayeb J. 1987; Expression of the art/trs protein of HIV and study of its role in viral envelope synthesis. Science 236:837–840
    [Google Scholar]
  23. Lewis N., Williams J., Rekosh D., Hammarskjöld M. L. 1990; Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV2) that is responsive to the HIVI rev and human T-cell leukemia virus types I and II rex proteins. Journal of Virology 64:1690–1697
    [Google Scholar]
  24. Linsley P. S., Ledbetter J. A., Kinney-Thomas E., Hu S. L. 1988; Effects of anti-gp 120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1. Journal of Virology 62:3695–3702
    [Google Scholar]
  25. Malim M. H., Hauber J., Le S. Y., Maizel J. V., Cullen B. R. 1989a; The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA. Nature, London 338:254–257
    [Google Scholar]
  26. Malim M. H., Bohnlein S., Fenrick R., Le S. Y., Maizel J. V., Cullen B. R. 1989b; Functional comparison of the Rev transactivators encoded by different primate immunodeficiency virus species. Proceedings of the National Academy of Sciences, U.S.A. 86:8222–8226
    [Google Scholar]
  27. Malim M. H., Tiley L. S., McCarn D. F., Rusche J. R., Hauber J., Cullen B. R. 1990; HIV1 structural gene expression requires binding of the Rev trans-activator to its RNA target sequence. Cell 60:675–683
    [Google Scholar]
  28. Nagashima K., Yoshida M., Seiki M. B. 1986; A single species of pX mRNA of human T-cell leukemia virus type I encodes trans-activator p40X and two other phosphoproteins. Journal of Virology 60:394–399
    [Google Scholar]
  29. Olsen H. S., Nelbock P., Cochrane A. W., Rosen C. A. 1990; Secondary structure is the major determinant for interaction of HIV rev protein with RNA. Science 247:845–848
    [Google Scholar]
  30. Rey M. -A., Krust B., Laurent A. G., Montagnier L., Hovanessian A. G. 1989; Characterization of human immunode-ficiency virus type 2 envelope glycoproteins: dimerization of the glycoprotein precursor during processing. Journal of Virology 63:647–658
    [Google Scholar]
  31. Rosen C. R., Terwilliger E., Dayton A. I., Sodroski J. G., Haseltine W. A. 1988; Intragenic cis-acting art gene-responsive sequences of the human immunodeficiency virus. Proceedings of the National Academy of Sciences, U.S.A. 85:2071–2075
    [Google Scholar]
  32. Sadaie M. R., Benter T., Wong-Staal F. 1988; Site-directed mutagenesis of two trans-regulatory genes (tat-III, trs) of HIV-1. Science 239:910–913
    [Google Scholar]
  33. Sakai H., Shibata R., Miura T., Hayami M., Ogawa K., Kiyomasu T., Ishimoto A., Adachi A. 1990; Complementation of the rev gene mutation among human and simian lentiviruses. Journal of Virology 64:2202–2207
    [Google Scholar]
  34. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  35. Schwartz S., Felber B. K., Benko D. M., Fenyö E. M., Pavlakis G. N. 1990; Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1. Journal of Virology 64:2519–2529
    [Google Scholar]
  36. Sodroski J., Goh W. C., Rosen C. A., Dayton A., Terwilliger E., Haseltine W. A. 1986; A second post-transcriptional transactivator gene required for the HTLV-III replication. Nature, London 321:412–117
    [Google Scholar]
  37. Terwilliger E., Burghoff R., Sia R., Sodroski J., Haseltine W. A., Rosen C. 1988; The art gene product of the human immunodeficiency virus is required for replication. Journal of Virology 62:655–658
    [Google Scholar]
  38. Wain-Hobson S., Sonigo P., Danos O., Alizon M. 1985; Nucleotide sequence of the AIDS virus, LAV. Cell 40:9–17
    [Google Scholar]
  39. Wain-Hobson S., Vartanian J. P., Henry M., Chenciner N., Cheynier R., Delassus S., Pedroza Martins L., Sala M., Nugeyre M.T., Guetard D., Klatzmann D., Montagnier L. 1991; LAV revisited: origins of the early HIV-1 isolates from Institut Pasteur. Science 252:961–965
    [Google Scholar]
  40. Zapp M. L., Green M. R. 1989; Sequence-specific RNA-binding by the HIV-1 Rev protein. Nature, London 342714–716
    [Google Scholar]
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