Experimental infection of cynomolgus and African green monkeys with human herpesvirus 6 Free

Abstract

Cynomolgus and African green monkeys were inoculated with human herpesvirus 6 (HHV-6). An antibody response was first observed 10 days and 5 days after inoculation of cynomolgus monkeys and African green monkeys, respectively, and was detectable for the duration of the experiment (33 days). HHV-6 DNA was first detected by the polymerase chain reaction in mononuclear cells of one cynomolgus monkey and one African green monkey 10 days after virus inoculation, and in a total of three of four cynomolgus monkeys (75%) and four of five African green monkeys (80%) later after inoculation. Furthermore, HHV-6 DNA was detected in the lymph nodes and spleen of monkeys killed 33 days after virus inoculation. A rash was observed on the trunk of one African green monkey 13 days after virus inoculation, otherwise the infection was asymptomatic. When mononuclear cells from both groups of monkeys were cultured in medium containing concanavalin A and interleukin 2, and infected with HHV-6 , virus replication was observed. The data suggest that HHV-6 infects these species of monkey and that this system could be useful as an animal model of HHV-6 infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-7-1673
1992-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/7/JV0730071673.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-7-1673&mimeType=html&fmt=ahah

References

  1. Asada H., Yalcin S., Balachandra K., Higashi K., Yamanishi K. 1989; Establishment of titration system for human herpesvirus 6 and evaluation of neutralizing antibody response to the virus. Journal of Clinical Microbiology 27:2204–2207
    [Google Scholar]
  2. Aubin J., Collandre H., Candotti D., Ingrand D., Rouzioux C., Burgard M., Richard S., Huraux J., Agut H. 1991; Several groups among human herpesvirus 6 strains can be distinguished by Southern blotting and polymerase chain reaction. Journal of Clinical Microbiology 29:367–372
    [Google Scholar]
  3. Buchbinder A., Josephs S. F., Ablashi D., Salahuddin S. Z., Klotman M. E., Manak M., Krueger G. R. F., Wong-Staal F., Gallo R. C. 1988; Polymerase chain reaction amplification and in situ hybridization for the detection of human B-lymphotropic virus. Journal of Virological Methods 21:191–197
    [Google Scholar]
  4. Downing R. G., Sewankambo N., Sewadda D., Honess R., Crawford D., Jarrett R., Griffin B. E. 1987; Isolation of human lymphotropic herpesviruses from Uganda. Lancet ii 390:
    [Google Scholar]
  5. Hellstrom B., Vahlquist B. 1951; Experimental inoculation of roseola infantum. Acta pediatrica scandinavica 40:189–197
    [Google Scholar]
  6. Higashi K., Asada H., Kurata T., Ishikawa K.-L., Hayami M., Spriatna Y., Sutarman Yamanishi K. 1989; Presence of antibody to human herpesvirus 6 in monkeys. Journal of General Virology 70:3171–3176
    [Google Scholar]
  7. Kempe C. H., Shaw E. B., Lackson J. R., Silver H. K. 1950; Studies on the etiology of exanthem subitum (roseola infantum). Journal of Pediatrics 37:561–568
    [Google Scholar]
  8. Kondo K., Hayakawa Y., Mori H., Sato S., Kondo T., Takahashi K., Minamishima Y., Takahashi M., Yamanishi K. 1990; Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum. Journal of Clinical Microbiology 28:970–974
    [Google Scholar]
  9. Kondo K., Kondo T., Okuno T., Takahashi M., Yamanishi K. 1991; Latent human herpesvirus 6 infection of human monocytes/ macrophages. Journal of General Virology 72:1401–1408
    [Google Scholar]
  10. Lawrence G. L., Chee M., Craxton M. A., Compels U. A., Honess R. W., Barrell B. G. 1990; Human herpesvirus 6 is closely related to human cytomegalovirus. Journal of Virology 64:287–299
    [Google Scholar]
  11. Lopez C., Pellett P., Stewart J., Goldsmith C., Sanderlin K., Black J., Warfield D., Feorini P. 1988; Characteristics of human herpesvirus 6. Journal of Infectious Diseases 157:1271–1273
    [Google Scholar]
  12. Lusso C., Markham P. D., Derocco S., Gallo R. C. 1990; In vitro susceptibility of T lymphocytes from chimpanzees (Pan troglodytes) to human herpesvirus 6 (HHV-6): a potential animal model to study the interaction between HHV-6 and human immunodeficiency virus type 1 in vitro. Journal of Virology 64:2751–2758
    [Google Scholar]
  13. Miyoshi L., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., Akagi T. 1982; Type C virus producing cell lines derived from adult T cell leukemia. GANN Monographs on Cancer Research 28:219–228
    [Google Scholar]
  14. Okuno T., Takahashi K., Balachandra K., Shiraki K., Yamanishi K., Takahashi M., Baba K. 1989; Seroepidemiology of human herpesvirus 6 infection in normal children and adults. Journal of Clinical Microbiology 4:651–653
    [Google Scholar]
  15. Okuno T., Sao H., Asada H., Shiraki K., Takahashi M., Yamanishi K. 1990; Analysis of a glycoprotein of human herpesvirus 6 (HHV-6) using monoclonal antibodies. Virology 176:625–628
    [Google Scholar]
  16. Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-staal F., Kramarsky B., Gallo R. C. 1986; Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science 234:596–601
    [Google Scholar]
  17. Schirmer E., Wyatt L., Yamanishi K., Rodriguez W., Frenkel N. 1991; Differentiation between two distinct classes of viruses now classified as human herpesvirus 6. Proceedings of the National Academy of Sciences, U.S.A. 88:5922–5926
    [Google Scholar]
  18. Tedder R. S., Briggs M., Cameron C. H., Honess R., Robertson D., Whittle H. 1987; A novel lymphotropic herpesvirus. Lancet ii:390–392
    [Google Scholar]
  19. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. 1988; Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet i1065–1067
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-7-1673
Loading
/content/journal/jgv/10.1099/0022-1317-73-7-1673
Loading

Data & Media loading...

Most cited Most Cited RSS feed