1887

Abstract

The nucleotide sequence of a 12 kbp dIII fragment (dIII C) from the right end of the unique component of the genome of human herpesvirus 6 (HHV-6) (strain U1102) was determined. The sequence has a mean G+C content of 42% and contains approximately 28 copies of a tandemly repeated 104 to 107 bp element, which, with a single exception, contain a cleavage site for I (the I repeats). Each of these elements contains potential binding sites for transcription factors NF-κB and AP2. The I repeats lie immediately upstream of a region previously identified as a candidate immediate early (IE) gene locus and therefore may constitute an IE gene enhancer element. One incomplete and six complete open reading frames (ORFs) were identified in the unique sequence of the dIII C fragment. The predicted products of these ORFs do not include homologues of proteins encoded by members of the alpha- or gammaherpesvirus subfamily. However, the dIII C fragment does contain a homologue of the US22 gene family, previously found only in the betaherpesvirus human cytomegalovirus (HCMV). These findings provide evidence that the close phylogenetic relationship between HHV-6 and HCMV is not confined to the betaherpesvirus-specific arrangement of conserved replicative and structural genes which has been demonstrated previously.

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1992-07-01
2022-10-07
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