1887

Abstract

Seed transmission of an isolate of pea seed-borne mosaic virus (PSbMV) in several pea genotypes has been studied. Cross-pollination experiments showed that pollen transmission of PSbMV did not occur and accordingly, virus was not detected in pollen grains by ELISA or electron microscopy. Comparative studies between two pea cultivars, one with a high incidence of seed transmission and one with none, showed that PSbMV infected the floral tissues (sepals, petals, anther and carpel) of both cultivars, but was not detected in ovules prior to fertilization. Virus was detected equally well in seed coats of the progeny in both cultivars. Analysis of virus incidence and concentration in pea seeds of different developmental stages demonstrated that in the cultivar with a high incidence of seed transmission, PSbMV directly invaded immature embryos, multiplied in the embryonic tissues and persisted during seed maturation. In contrast, the cultivar without seed transmission did not show invasion of immature embryos by the virus; there was no evidence for virus multiplication or persistence during embryo development and seed maturation. Hence seed transmission of PSbMV resulted from direct invasion of immature pea embryos by the virus and the block to seed transmission in the nonpermissive cultivar probably occurred at this step.

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1992-07-01
2024-04-25
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References

  1. Bennett C. W. 1969; Seed transmission of plant viruses. Advances in Virus Research 14:221–261
    [Google Scholar]
  2. Bos L. 1977; Seed borne viruses. In Plant Health and Quarantine in International Transfer of Genetic Resources pp 39–65 Edited by Hewitt W. B., Chiarappa L. Boca Raton: CRC Press;
    [Google Scholar]
  3. Bowers G. R. Jr, Goodman R. M. 1979; Soybean mosaic virus: infection of soybean seed parts and seed transmission. Phytopathology 69:569–572
    [Google Scholar]
  4. Carroll T. W. 1981; Seedbome viruses: virus-host interactions. In Plant Diseases and Vectors pp 293–317 Edited by Maramorosch K., Harris K. F. New York & London: Academic Press;
    [Google Scholar]
  5. Casey R. 1990; Regulation of gene expression during development. In Plant Physiology, Biochemistry and Molecular Biology pp 16–27 Edited by Dennis D. T., Turpin D. H. Harlow: Longmans;
    [Google Scholar]
  6. Casey R., Domoney C., Nielsen N. C. 1985; Isolation of a cDNA clone for pea (Pisum sativum) seed lipoxygenase. Biochemical Journal 232:79–85
    [Google Scholar]
  7. Cockbain A. J., Ding X. S., Wang D., Biddle A. J. 1988; Pea seed-bome mosaic virus (PSbMV). Rothamsted Experimental Station Annual Report for 1987 pp 70–71
    [Google Scholar]
  8. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  9. Irwin M. E., Goodman R. M. 1981; Ecology and control of soybean mosaic virus. In Plant Diseases and Vectors pp 181–220 Edited by Maramorosch K., Harris K. F. New York & London: Academic Press;
    [Google Scholar]
  10. Johansen E., Ramussen O. F., Heide M., Borkhardt B. 1991; The complete nucleotide sequence of pea seed-bome mosaic virus RNA. Journal of General Virology 72:2625–2632
    [Google Scholar]
  11. Khetarpal R. K., Maury Y. 1987; Pea seed-bome mosaic vims: a review. Agronomic 7:215–224
    [Google Scholar]
  12. Medina A. C., Grogan R. G. 1961; Seed transmission of bean mosaic viruses. Phytopathology 51:452–456
    [Google Scholar]
  13. Nelson R., Down E. E. 1933; Influence of pollen and ovule infection in seed transmission of bean mosaic. Phytopathology23–25
    [Google Scholar]
  14. Phatak H. C. 1974; Seed-bome plant viruses – identification and diagnosis in seed health testing. Seed Science Technology 2:3–155
    [Google Scholar]
  15. Reddick D., Stewart V. B. 1919; Transmission of the vims of bean mosaic in seed and observations on thermal death point of seed and vims. Phytopathology 9:445–450
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Schippers B. 1963; Transmission of bean common mosaic vims by seed of Phaseolus vulgaris L. cultivar Beka. Acta botanica neerlanda 12:433–497
    [Google Scholar]
  18. Shepherd R. J. 1972; Transmission of vimses through seed and pollen. In Principles and Techniques in Plant Virology pp 267–292 Edited by Kado C. I., Agrawal H. O. New York: Van Nostrand Reinhold;
    [Google Scholar]
  19. Stevenson W. R., Hagedorn D. J. 1969; A new seed-bome vims of peas. Phytopathology 59:1051–1052
    [Google Scholar]
  20. Stevenson W. R., Hagedorn D. J. 1973; Further studies on seed transmission of pea seed-bome mosaic vims in Pisum sativum. Plant Disease Reporter 57:248–252
    [Google Scholar]
  21. Wang D., Woods R. D., Swaby A. G., Cockbain A. J. 1991; Detection of pea seed-bome mosaic vims virion and pinwheel inclusion body proteins in leaf and seed tissues of pea (Pisum sativum L.) by immunogold labelling. Agronomic 11:387–394
    [Google Scholar]
  22. Wang D., Hayes I. M., Maule A. J. 1992a; Procedures for the efficient purification of pea seed-bome mosaic vims and its genomic RNA. Journal of Virological Methods 36:223–230
    [Google Scholar]
  23. Wang D., Maule A. J., Biddle A. J., Woods R. D., Cockbain A. J. 1992b; The susceptibility of pea cultivars to pea seed-bome mosaic vims infection and vims seed transmission in the U.K.. Plant Pathology (in press)
    [Google Scholar]
  24. Xu Z., Chen K., Zhang Z., Chen J. 1991; Seed transmission of peanut stripe vims in peanut. Plant Disease 75723–726
    [Google Scholar]
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