1887

Abstract

The nucleotide sequence of bovine respiratory syncytial virus (RSV), ATCC strain A51908 fusion (F) glycoprotein gene cDNA was determined. The amino acid sequence deduced was then compared to those of two different isolates of bovine RSV, strains RB 94 and 391–2, and the A and B subtypes of human RSV, strains 18537 and A2. The bovine RSV F protein is highly conserved between the three isolates, A51908 has 97% amino acid identity to RB 94, and 99% identity to 391–2. The F proteins of both the A and B types of human RSV are 81% identical to that of A51908. The cDNA clone was expressed using a baculovirus vector and the expressed recombinant F protein produced in SF9 cells was characterized by Western blot analysis. The recombinant F protein was post-translationally cleaved into the active form and reacted with serum from bovine RSV-infected calves.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-6-1563
1992-06-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/6/JV0730061563.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-6-1563&mimeType=html&fmt=ahah

References

  1. Burand J. P., Summers M. D., Smith G. E. 1980; Transfection with baculovirus DNA. Virology 101:286–290
    [Google Scholar]
  2. Cochran M., Erickson B., Knell J., Smith G. 1987; Use of baculovirus recombination as a general method for production of subunit vaccines. In Vaccines 1987 pp 384–388 Edited by Chanock R. M. et al New York: Cold Spring Harbor Laboratories;
    [Google Scholar]
  3. Gruber C., Levine S. 1983; Respiratory syncytial virus polypeptides. III. The envelope-associated proteins. Journal of General Virology 64:825–832
    [Google Scholar]
  4. Gubler U. 1987; Second-strand cDNA synthesis: classical method. Methods in Enzymology 152:325–335
    [Google Scholar]
  5. Johnson P. R., Collins P. L. 1988; The fusion glycoprotein of human respiratory syncytial viruses of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  6. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. 1969; Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–434
    [Google Scholar]
  7. Kimman T. G., Sol J., Westenbrink F., Straver P. J. 1989; A severe outbreak of respiratory tract disease associated with bovine respiratory syncytial virus probably enhanced by vaccination with modified live vaccine. Veterinary Quarterly 11:250–253
    [Google Scholar]
  8. Lerch R. A., Anderson K., Wertz G. W. 1990; Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. Journal of Virology 64:5559–5569
    [Google Scholar]
  9. Lerch R. A., Anderson K., Wertz G. W. 1991; Nucleotide sequence analysis of the bovine respiratory syncytial virus fusion protein mRNA and expression from a recombinant vaccinia virus. Virology 181:118–131
    [Google Scholar]
  10. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  11. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Chanock R. M., Collins P. L. 1986; Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of F and G glycoproteins to host immunity. Proceedings of the National Academy of Sciences, U,. S,. A. 83:7462–7466
    [Google Scholar]
  12. Pemberton R. M., Cannon M. J., Openshaw P. J. M., Ball L. A., Wertz G. W., Askonas B. A. 1987; Cytotoxic T cell specificity for respiratory syncytial virus proteins: fusion protein is an important target antigen. Journal of General Virology 68:2177–2182
    [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A 74:5463–5467
    [Google Scholar]
  14. Stott E. J., Taylor G. 1985; Respiratory syncytial virus. Brief review. Archives of Virology 84:1–52
    [Google Scholar]
  15. Villareal L. P., Berg P. 1977; Hybridization in situ of SV40 plaques: detection of recombinant SV40 virus carrying specific sequences of nonviral DNA. Science 196:183–185
    [Google Scholar]
  16. Walravens K., Kettmann R., Collard A., Coppe P., Burny A. 1990; Sequence comparison between the fusion protein of human and bovine respiratory syncytial viruses. Journal of General Virology 71:3009–3014
    [Google Scholar]
  17. Walsh E. E., Hall C. B., Briselli M., Brandriss M. W., Schlesinger J. J. 1987; Immunization with glycoprotein subunits of respiratory syncytial virus to protect cotton rats against viral infection. Journal of Infectious Diseases 155:1198–1204
    [Google Scholar]
  18. Wathen M. W., Brideau R. J., Thomsen D. R. 1989; Immunization of cotton rats with the human respiratory syncytial virus F glycoprotein produced using a baculovirus vector. Journal of Infectious Diseases 159:255–263
    [Google Scholar]
  19. Wright P. F., Belshe R. B., Kim H. W., Van Voris L. P., Chanock R. M. 1982; Administration of a highly attenuated, live respiratory syncytial virus vaccine to adults and children. Infection and Immunity 37397–400
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-6-1563
Loading
/content/journal/jgv/10.1099/0022-1317-73-6-1563
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error