Bovine papillomavirus type 1-transformed primary mouse fibroblasts show no correlation between tumorigenicity and viral gene expression, but c- gene expression is elevated in tumorigenic cell lines Free

Abstract

Bovine papillomavirus type 1 (BPV-1)-transformed primary mouse fibroblasts containing episomal or integrated BPV-1 sequences were analysed for virus-specific transcripts and c- gene expression. Total BPV-1-specific expression was high in cell lines containing episomal BPV-1 DNA in comparison to lines containing integrated BPV-1 sequences, mainly due to higher expression of the E6/E7 sequences. No correlation was found between the viral transcription and tumorigenicity, although BPV-1 gene expression occurred in all cell lines. High levels of c- expression were found in all cell lines exhibiting a tumorigenic phenotype as compared to the non-tumorigenic lines. These data suggest that expression of BPV-1 genes may be essential for transformation but not tumorigenicity, whereas high levels of expression of cellular oncogenes like c- may be associated with tumorigenicity.

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1992-06-01
2024-03-28
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References

  1. Agrawal R., Pelkonen J., Rytkönen M., Mäntyjärvi R. A. 1992; Integration of bovine papillomavirus type 1 DNA and analysis of the amplified virus–cell junctions in transformed primary mouse fibroblasts. Journal of General Virology 73:201–206
    [Google Scholar]
  2. Baker C. C. 1990; Bovine papillomavirus type 1 transcription. In Papillomaviruses and Human Cancer pp 91–112 Edited by Pfister H. Boca Raton: CRC Press;
    [Google Scholar]
  3. Baker C. C., Howley P. M. 1987; Differential promoter utilization by the bovine papillomavirus in transformed cells and productively infected wart tissues. EMBO Journal 6:1027–1035
    [Google Scholar]
  4. Bergman P., Ustav M., Sedman J., Moreno-Lopez J., Venn-strom B., Pettersson U. 1988; The E5 gene of bovine papillomavirus type 1 is sufficient for complete oncogenic transformation of mouse fibroblasts. Oncogene 2:453–459
    [Google Scholar]
  5. Binétruy B., Meneguzzi G., Breathnach R., Cuzin F. 1982; Recombinant DNA molecules comprising bovine papillomavirus type 1 DNA linked to plasmid DNA are maintained in a plasmidial state both in rodent fibroblasts and in bacterial cells. EMBO Journal 1:621–628
    [Google Scholar]
  6. Binétruy B., Schiller J., Lowy D., Cerni C., Cuzin F. 1990; Non-selective analysis of the transformation of FR3T3 rat cells by bovine papillomavirus type 1: regulations of viral transcription associated with phenotypic transformation. Oncogene 5:1645–1651
    [Google Scholar]
  7. Burnett S., MorenoLopez J., Pettersson U. 1987; Messenger RNAs from the El region of bovine papillomavirus type 1 detected in virus-infected bovine cells. Nucleic Acids Research 15:8607–8620
    [Google Scholar]
  8. Chen L., Thomas E. K., Hu S.-L., HellströM I., Hellström K. E. 1991; Human papillomavirus type 16 nucleoprotein E7 is a tumor rejection antigen. Proceedings of the National Academy of Sciences, U.S.A. 88:110–114
    [Google Scholar]
  9. Couturier J., Sastre–Garau X., Schneider-Maunoury S., Labib A., Orth G. 1991; Integration of papillomavirus DNA near myc genes in genital carcinomas and its consequences for proto-oncogene expression. Journal of Virology 65:4534–4538
    [Google Scholar]
  10. DiMaio D., Treisman R. H., Maniatis T. 1982; Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells. Proceedings of the National Academy of Sciences, U.S.A. 79:4030–4034
    [Google Scholar]
  11. Doeberitz M. K., Bauknecht T., Bartsch D., zur Hausen H. 1991; Influence of chromosomal integration on glucocorticoid-regulated transcription of growth-stimulating papillomavirus genes E6 and E7 in cervical carcinoma cells. Proceedings of the National Academy of Sciences, U.S.A. 88:1411–1415
    [Google Scholar]
  12. Fort P., Marty L., Piechaczyk M., Sabrouty S. E., Dani C., Jeanteur P., Blanchard J. M. 1985; Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Research 13:1431–1442
    [Google Scholar]
  13. Heilman C. A., Engel L., Lowy D. R., Howley P. M. 1982; Virus-specific transcription in bovine papillomavirus-transformed mouse cells. Virology 119:22–34
    [Google Scholar]
  14. Howley P. M. 1983; The molecular biology of papillomavirus transformation. American Journal of Pathology 113:414–421
    [Google Scholar]
  15. Jaureguiberry G., Favre M., Orth G. 1983; Bovine papilloma-virus type 1 genome in hamster sarcoma cells in vivo and in vitro: variation in the level of transcription. Journal of General Virology 64:1199–1204
    [Google Scholar]
  16. Laatikainen A., Karjalainen H., Jägerroos H., Sarkkinen H., Mäntyjärvi R. 1990; Tumorigenicity and H-2 expression of papillomavirus-transformed mouse cell lines. Cancer Immunology and Immunotherapy 31:164–168
    [Google Scholar]
  17. Lambert P. F., Spalholz B. A., Howley P. M. 1987; A transcriptional repressor encoded by BPV-1 shares a common carboxy terminal domain with the E2 transactivator. Cell 50:69–78
    [Google Scholar]
  18. Lambert P. F., Baker C. C., Howley P. M. 1988; The genetics of bovine papillomavirus type 1. Annual Review of Genetics 22:235–258
    [Google Scholar]
  19. Lancaster W., Olson C. 1982; Animal papillomaviruses. Microbiological Reviews 46:191–207
    [Google Scholar]
  20. Land H., Parada L. F., Weinberg R. A. 1983; Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature, London 304:596–602
    [Google Scholar]
  21. Leptak C., Cajal S. R., Kulke R., Horwitz B. H., Riese D. J. II, Dotto G. P., DiMaio D. 1991; Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16. Journal of Virology 65:7078–7083
    [Google Scholar]
  22. Lusky M., Botchan M. R. 1984; Characterisation of the bovine papillomavirus plasmid maintenance sequences. Cell 36:391–401
    [Google Scholar]
  23. Lusky M., Botchan M. R. 1986; Transient replication bovine papillomavirus type 1 plasmids: cis and trans requirements. Proceedings of the National Academy of Sciences, U.S.A. 83:3609–3613
    [Google Scholar]
  24. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Mäntyjärvi R., Sarkkinen H., Parkkinen S., Ryhänen A., Karjalainen H., Syrjänen K., Syrjänen S. 1988; Phenotypic transformation of primary mouse fibroblasts by BPV–1 DNA. Archives of Virology 100:17–25
    [Google Scholar]
  26. Neary K., DiMaio D. 1989; Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells. Journal of Virology 63:259–266
    [Google Scholar]
  27. Schiller J. T., Vass W. C., Lowy D. R. 1984; Identification of a second transforming region on bovine papillomavirus DNA. Proceedings of the National Academy of Sciences, U.S.A. 81:7880–7884
    [Google Scholar]
  28. Schiller J. T., Vass W. C., Vousden K. H., Lowy D. R. 1986; E5 open reading frame of bovine papillomavirus type 1 encodes a transforming protein. Science 233:464–466
    [Google Scholar]
  29. Smith K. T., Campo M. S. 1988; ‘Hit and run’ transformation of mouse C127 cells by bovine papillomavirus type 4: the viral DNA is required for initiation but not for maintenance of the transformed phenotype. Virology 164:39–47
    [Google Scholar]
  30. Smith K. T., Campo M. S. 1989; Amplification of specific DNA sequences in Cl27 mouse cells transformed by bovine papillomavirus type 4. Oncogene 4:409–413
    [Google Scholar]
  31. Sousa R., Dostatni N., Yaniv M. 1990; Control of papillomavirus gene expression. Biochimica et biophysica acta 1032:19–37
    [Google Scholar]
  32. Spalholz B. A., Yang Y.-C., Howley P. M. 1985; Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product. Cell 42:183–191
    [Google Scholar]
  33. Spalholz B. A., Lambert P. F., Yee C. L., Howley P. M. 1987; Bovine papillomavirus transcriptional regulation: localization of the E2-responsive elements of the long control region. Journal of Virology 61:2128–2137
    [Google Scholar]
  34. Stenlund A., Zabielski J., Ahola H., Moreno-Lopez J., Pettersson U. 1985; Messenger RNAs from the transforming region of bovine papilloma virus type 1. Journal of Molecular Biology 182:541–554
    [Google Scholar]
  35. Tada A., Sekine H., Yamamoto T., Fuse A., Simizu B. 1989; Characterization of bovine papillomavirus type 1-transformed clones which show distinct transformed phenotypes. Journal of General Virology 70:1593–1599
    [Google Scholar]
  36. Yang Y.-C., Okayama H., Howley P. M. 1985; Bovine papillomavirus contains multiple transforming genes. Proceedings of the National Academy of Sciences, U.S.A. 82:1030–1034
    [Google Scholar]
  37. Zhang Y.-L., Lewis A. Jr, Wade-Glass M., Schlegel R. 1987; Levels of bovine papillomavirus RNA and protein expression correlate with variations in the tumorigenic phenotype of hamster cells. Journal of Virology 612924–2928
    [Google Scholar]
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