1887

Abstract

The temporal expression of the multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes in cells was studied using virus recombinants in which either gene was replaced by the juvenile hormone esterase (JHE) gene of . The JHE served as a highly specific and sensitive reporter for gene expression. Activation of the p10 gene followed a pattern different to that of polyhedrin. The p10 gene was activated a few hours earlier than the polyhedrin gene, but its expression reached a lower maximum level. Northern blot analysis complemented and confirmed the results obtained from the JHE assays. Co-infection of sense recombinants and those containing an anti-sense copy of the JHE gene in place of the polyhedrin or p10 gene resulted in reduced levels of JHE gene expression. These experiments independently supported the hypothesis that the p10 gene promoter is more active at earlier times post-infection than that of the polyhedrin gene. The results also highlight the potential of the antisense strategy as an experimental approach for the study of baculovirus gene regulation and possibly insect metabolism.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-6-1481
1992-06-01
2022-09-30
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/6/JV0730061481.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-6-1481&mimeType=html&fmt=ahah

References

  1. Abdel-Aal Y. A. I., Hammock B. D. 1985; 3-octylthio-l, l, l-trifluoro-2-propanone, a high affinity and slow binding inhibitor of juvenile hormone esterase from Trichoplusia ni (Hubner). Insect Biochemistry 15:111–122
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction method for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
    [Google Scholar]
  4. Chang L.-J., Stoltzfus M. 1985; Gene expression from intronless and intron-containing Rous sarcoma virus clones is specifically inhibited by anti-sense RNA. Molecular and Cellular Biology 5:2341–2348
    [Google Scholar]
  5. Crowley T. E., Nellen W., Gomer R. H., Firtel R. A. 1985; Phenocopy of discoidin 1-minus mutants by antisense transformation in Dictyostelium. Cell 43:633–641
    [Google Scholar]
  6. Cuozzo M., O’Connell K. M., Kaniewski W., Fang R.-X, Chua N.-H., Tumer N. E. 1988; Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio I Technology 6:549–557
    [Google Scholar]
  7. Fraser M. J., Brusca J. S., Smith G. E., Summers M. D. 1985; Transposon mediated mutagenesis of a baculovirus. Virology 145:356–361
    [Google Scholar]
  8. Friesen P. D., Miller L. K. 1986; The regulation of baculovirus gene expression. Current Topics in Microbiology and Immunology 131:31–49
    [Google Scholar]
  9. Granados R. R., Federici B. A. (editors) 1986; The Biology of Baculoviruses. vol 1 Biological Properties and Molecular Biology Boca Raton: CRC Press;
    [Google Scholar]
  10. Green P. J., Pines O., Inouye M. 1986; The role of antisense RNA in gene regulation. Annual Review of Biochemistry 55:569–597
    [Google Scholar]
  11. Hammock B. D., Roe R. M. 1985; Analysis of juvenile hormone esterase activity. Methods in Enzymology 111:487–494
    [Google Scholar]
  12. Hammock B. D., Bonning B. C., Possee R. D., Hanzlik T. N., Maeda S. 1990; Expression and effects of the juvenile hormone esterase in a baculovirus vector. Nature, London 344:458–460
    [Google Scholar]
  13. Hanzlik T. N., Hammock B. D. 1987; Characterization of affinity purified juvenile hormone esterase from Trichoplusia ni. Journal of Biological Chemistry 262:13584–13591
    [Google Scholar]
  14. Hanzlik T. N., Abdel-Aal Y. A. I., Harshman L. G., Hammock B. D. 1989; Isolation and sequencing of cDNA clones coding for juvenile hormone esterase from Heliothis virescens. Journal of Biological Chemistry 21:12419–12425
    [Google Scholar]
  15. Hemenway C., Fang R.-X., Kaniewski W. K., Chua N.-H., Tumer N. E. 1988; Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO Journal 7:1273–1280
    [Google Scholar]
  16. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni. Nature, London 226:466–467
    [Google Scholar]
  17. Izant J. G., Weintraub H. 1984; Inhibition of thymidine kinase gene expression by antisense RNA: a molecular approach to genetic analysis. Cell 36:1007–1015
    [Google Scholar]
  18. Jarvis D. L., Summers M. D. 1989; Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Molecular and Cellular Biology 9:214–223
    [Google Scholar]
  19. Kelly D. C., Lescott T. 1981; Baculovirus replication: protein synthesis in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. Microbiologica 4:35–47
    [Google Scholar]
  20. Kim S. K., Wold B. J. 1985; Stable reduction of thymidine kinase activity in cells expressing high levels of antisense RNA. Cell 42:129–138
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. Luckow V. L., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Bio/Technology 6:47–55
    [Google Scholar]
  23. Maeda S. 1989; Expression of foreign genes in insects using baculovirus vectors. Annual Review of Entomology 34:351–372
    [Google Scholar]
  24. Maruniak J. W., Summers M. D. 1981; Autographa californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. Virology 109:25–34
    [Google Scholar]
  25. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  26. Miller L. K. 1988; Baculoviruses as gene expression vectors. Annual Review of Microbiology 42:177–199
    [Google Scholar]
  27. Min M. K., Bishop D. H. L. 1991; Transcriptional analyses of baculovirus polyhedrin and foreign gene expression relative to baculovirus p10 mRNA levels. Journal of General Virology 72:2551–2556
    [Google Scholar]
  28. Mizuno T., Chou M.–Y., Inouye M. 1984; A unique mechanism regulating gene expression; translational inhibition by a complementary RNA transcript (micRNA). Proceedings of the National Academy of Sciences, U.S.A. 82:1966–1970
    [Google Scholar]
  29. Murphy C. L., Lennick M., Lehar S. M., Beltz G. A., Young E. 1990; Temporal expression of HIV-1 envelope proteins in baculovirus-infected insect cells: implications for glycosylation and CD4 binding. Genetic Analysis: Techniques and Applications 7:160–171
    [Google Scholar]
  30. Ooi B. G., Miller L. K. 1990; Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream. Journal of Virology 64:3126–3129
    [Google Scholar]
  31. Ooi B. G., Rankin C., Miller L. K. 1989; Downstream sequences augment transcription from the essential initiation site of a baculovirus polyhedrin gene. Journal of Molecular Biology 210:721–736
    [Google Scholar]
  32. Possee R. D., Howard S. C. 1987; Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus. Nucleic Acids Research 15:10233–10248
    [Google Scholar]
  33. Powell P. A., Stark D. A., Sanders P. R., Beachy R. N. 1989; Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA. Proceedings of the National Academy of Sciences, U.S.A. 86:6949–6952
    [Google Scholar]
  34. Proudfoot N. J., Brownlee G. G. 1976; 3′ Non-coding region sequences in eukaryotic messenger RNA. Nature, London 263:211–214
    [Google Scholar]
  35. Qin J., Liu A., Weaver R. F. 1989; Studies on the control region of the p10 gene of Autographa californica nuclear polyhedrosis virus. Journal of General Virology 70:1273–1279
    [Google Scholar]
  36. Rankin C., Ooi B. G., Miller L. K. 1988; Eight base pairs encompassing the transcriptional start point are the major determinant for baculovirus polyhedrin gene expression. Gene 70:39–49
    [Google Scholar]
  37. Rohel D. Z., Cochran M. A., Faulkner P. 1983; Characterization of two abundant mRNAs of Autographa californica nuclear polyhedrosis virus present late in infection. Virology 124:357–365
    [Google Scholar]
  38. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  39. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning. A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. Smith G. E., Summers M. D. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology 98:517–527
    [Google Scholar]
  41. Smith G. E., Vlak J. M., Summers M. D. 1982; In vitro translation of Autographa californica nuclear polyhedrosis virus early and late mRNAs. Journal of Virology 44:199–208
    [Google Scholar]
  42. Smith G. E., Vlak J. M., Summers M. D. 1983; Physical analysis of Autographa californica nuclear polyhedrosis virus transcripts for polyhedrin and 10000-molecular weight protein. Journal of Virology 45:215–225
    [Google Scholar]
  43. van der Wilk F., van Lent J. W. M., Vlak J. M. 1987; Immunogold detection of polyhedrin, p10 and virion antigens in Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells. Journal of General Virology 68:2615–2623
    [Google Scholar]
  44. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  45. Vlak J. M. 1979; The proteins of nonoccluded Autographa californica nuclear polyhedrosis virus produced in an established cell line of Spodoptera frugiperda. Journal of Invertebrate Pathology 34:110–118
    [Google Scholar]
  46. Vlak J. M., Klinkenberg F. A., Zaal K. J. M., Usmany M., Klinge-Roode E. C., Geervliet J. B. F., Roosien J., van Lent J. W. M. 1988; Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expressing a p 10–β-galactosidase fusion gene. Journal of General Virology 69:765–776
    [Google Scholar]
  47. Vlak J. M., Schouten A., Usmany M., Belsham G. J., Klinge-Roode E. C., Maule A., van Lent J. W. M., Zuidema D. 1990; Expression of a cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. Virology 178:312–320
    [Google Scholar]
  48. Volkmann L. E., Keddie B. A. 1990; Nuclear polyhedrosis virus pathogenesis. Seminars in Virology 1:249–256
    [Google Scholar]
  49. Weyer U., Possee R. D. 1988; Functional analysis of the p10 gene 5′ leader sequence of the Autographa californica nuclear polyhedrosis virus p10 gene. Nucleic Acids Research 16:3635–3653
    [Google Scholar]
  50. Weyer U., Possee R. D. 1989; Analysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene. Journal of General Virology 70:203–208
    [Google Scholar]
  51. Weyer U., Knight S., Possee R. D. 1990; Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectors. Journal of General Virology 71:1525–1534
    [Google Scholar]
  52. Williams G. V., Rohel D. Z., Kuzio J., Faulkner P. 1989; A cytopathological investigation of Autographa californica nuclear polyhedrosis virus p10 gene function using insertion/deletion mutants. Journal of General Virology 70:187–202
    [Google Scholar]
  53. Zuidema D., Klinge-Roode E. C., van Lent J. W. M., Vlak J. M. 1989; Construction and analysis of an Autographa californica nuclear polyhedrosis virus mutant lacking the polyhedral envelope. Virology 173:98–108
    [Google Scholar]
  54. Zuidema D., Schouten A., Usmany M., Maule A. J., Belsham G. J., Roosien J., Klinge-Roode E. C., van Lent J. W. M., Vlak J. M. 1990; Expression of cauliflower mosaic virus gene I using a novel polyhedron-based baculovirus expression vector. Journal of General Virology 71:2201–2209
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-6-1481
Loading
/content/journal/jgv/10.1099/0022-1317-73-6-1481
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error