1887

Abstract

The adenovirus (Ad) hexon contains both group- and type-specific antigenic determinants. To identify the latter, peptides were synthesized corresponding to residues 281 to 292 from loop 1 and 441 to 455 from loop 2 of the Ad2 hexon. These sequences display type-specific variation and have been shown by X-ray crystallography to be present on the surface of the virion. Antisera raised against the peptides bound both peptide and the native hexon in ELISA, and blocked virus infectivity, as determined by immunofluorescence or neutralization assays. The loop 1 peptide was shown to inhibit binding of the corresponding antiserum to the native hexon in ELISA and to abolish its neutralizing activity. Neither the loop 1- nor loop 2-specific antiserum neutralized the infectivity of Ad4 or Ad40. Neutralization did not appear to result from aggregation of virus particles and thus their inability to attach to the cell, because virions treated with immune serum were internalized to the same extent as those treated with preimmune serum. Examination of the immune response elicited by Ad2 infection revealed that antibodies directed against the L1 and L2 epitopes were also present in human serum. Thus, the variable regions exposed on the surface of the Ad2 hexon represent type-specific neutralizing antigenic determinants.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-6-1429
1992-06-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/6/JV0730061429.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-6-1429&mimeType=html&fmt=ahah

References

  1. Adam E., Lengyel A., Takacs M., Erdei J., Fachet J., Nasz I. 1986; Grouping of monoclonal antibodies to adenovirus hexons by their cross-reactivity. Archives of Virology 87:61–71
    [Google Scholar]
  2. Atherton E., Cameron L. R., Sheppard R. C. 1988; Peptide synthesis. Part 10. Use of pentafluorophenyl esters of fluorenyl methoxycarbonylamino acids in solid phase synthesis. Tetrahedron 44:843–857
    [Google Scholar]
  3. Ginsberg H. S. 1984 The Adenoviruses New York: Plenum Press;
    [Google Scholar]
  4. Haase A. T., Pereira H. G. 1972; The purification of adenovirus neutralizing antibody: adenovirus type 5 hexon immunoadsorbent. Journal of Immunology 108:633–636
    [Google Scholar]
  5. Hay R. T., Stow N. D., McDougall I. M. 1984; Replication of adenovirus mini-chromosomes. Journal of Molecular Biology 175:493–510
    [Google Scholar]
  6. Hayashi K., Russell W. C. 1968; A study of the development of adenovirus antigens by the immunofluorescent technique. Virology 34:470–480
    [Google Scholar]
  7. Hierholzer J. C., Wigand R., Anderson L. J., Adrian T., Gold J. W. M. 1988; Adenoviruses from patients with AIDS: a plethora of serotypes and a description of five new serotypes of subgenus D (type 43-47). Journal of Infectious Diseases 158:804–813
    [Google Scholar]
  8. Kinloch R., Mackay N., Mautner V. 1984; Adenovirus hexon. Sequence comparison of subgroup C serotypes 2 and 5. Journal of Biological Chemistry 259:6431–6436
    [Google Scholar]
  9. Kjellen L., Pereira H. G. 1968; Role of adenovirus antigens in the induction of virus neutralizing antibody. Journal of General Virology 2:177–185
    [Google Scholar]
  10. Mautner V., Willcox H. N. A. 1974; Adenovirus antigens: a model system in mice for subunit vaccination. Journal of General Virology 25:325–336
    [Google Scholar]
  11. Norrby E. 1968; Comparative studies on the soluble components of adenovirus types 9 and 15 and the intermediate strain 9-15. Journal of Virology 2:1200–1207
    [Google Scholar]
  12. Norrby E. 1969a; The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology 37:565–576
    [Google Scholar]
  13. Norrby E. 1969b; The structural and functional diversity of adenovirus capsid components. Journal of General Virology 5:221–236
    [Google Scholar]
  14. Norrby E., Wadell G. 1969; Immunological relationship between hexons of certain human adenoviruses. Journal of Virology 4:663–670
    [Google Scholar]
  15. Patterson S., Russell W. C. 1983; Ultrastructural and immunofluorescence studies of early events in adenovirus-HeLa cell interactions. Journal of General Virology 64:1091–1099
    [Google Scholar]
  16. Pereira H. G., Valentine R. C., Russell W. C. 1968; Crystallization of an adenovirus protein (the hexon). Nature, London 219:946–947
    [Google Scholar]
  17. Philipson L. 1983; Structure and assembly of adenoviruses. Current Topics in Microbiology and Immunology 109:1–52
    [Google Scholar]
  18. Philipson L., Lonberg-Holm K., Pettersson U. 1968; Virus receptor interaction in an adenovirus system. Journal of Virology 2:1064–1075
    [Google Scholar]
  19. Roberts M. M., White J. L., Grütter M. G., Burnett R. M. 1986; Three-dimensional structure of the adenovirus major coat protein hexon. Science 232:1148–1151
    [Google Scholar]
  20. Russell W. C., Patel G., Precious B., Sharp I., Gardner P. S. 1981; Monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. Journal of General Virology 56:393–408
    [Google Scholar]
  21. Toogood C. I. A., Hay R. T. 1988; DNA sequence of the adenovirus type 41 hexon gene and predicted structure of the protein. Journal of General Virology 69:2291–2301
    [Google Scholar]
  22. Toogood C. I. A., Murali R., Burnett R. M., Hay R. T. 1989; The adenovirus type 40 hexon: sequence, predicted structure and relationship to other adenovirus hexons. Journal of General Virology 70:3203–3214
    [Google Scholar]
  23. Varga M. J., Bergman T., Everitt E. 1990; Antibodies with specificities against a dispase-produced 15-kilodalton hexon fragment neutralize adenovirus type 2 infectivity. Journal of Virology 64:4217–4225
    [Google Scholar]
  24. Willcox N., Mautner V. 1976a; Antigenic determinants of adenovirus capsids. I. Measurement of antibody cross reactivity. Journal of Immunology 116:19–24
    [Google Scholar]
  25. Willcox N., Mautner V. 1976b; Antigenic determinants of adenovirus capsids. II. Homogeneity of hexons, and accessibility of their determinants, in the virion. Journal of Immunology 116:25–29
    [Google Scholar]
  26. Wohlfart C. 1988; Neutralization of adenoviruses: kinetics, stoichiometry, and mechanisms. Journal of Virology 62:2321–2328
    [Google Scholar]
  27. Wohlfart C., Svensson U. K., Everitt E. 1985; Interaction between HeLa cells and adenovirus type 2 virions neutralized by different antisera. Journal of Virology 56:896–903
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-6-1429
Loading
/content/journal/jgv/10.1099/0022-1317-73-6-1429
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error