1887

Abstract

The role of natural killer (NK) cells in the early immune response to a pancreatropic isolate of coxsackievirus B4 (CVB4) was investigated in a murine model of pancreatitis. Endogenous (background) NK cell activity in fresh spleen effector cells from eight mouse strains was compared with virus-augmented NK cell activity 4 days post-infection (p.i.). A significant virus-induced increase ( < 0.003) in NK cell activity was seen in seven of eight infected mouse strains, when virus titres in the pancreas were beginning to fall. Lesions in the exocrine pancreas were least extensive in the three strains with the highest endogenous NK cell activity. In C3H/HeJ mice that had been depleted of NK cells prior to infection with a low virus concentration, resistance to infection of the pancreas was completely abolished; myocarditis was also observed in one of these animals. Thus, NK cells may limit virus replication in the pancreas and play a role in resistance in C3H/HeJ mice. Virus-specific neutralizing antibody was not detected in the serum until 5 to 6 days p.i. in most strains and did not appear to influence pancreatic virus titres. It may be significant that CVB4 infection did not induce the expression of major histocompatibility complex (MHC) class I molecules on target acinar cells. With certain tumour cells, an inverse relationship between MHC class I expression and susceptibility to NK cell-mediated lysis is well documented.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-6-1379
1992-06-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/6/JV0730061379.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-6-1379&mimeType=html&fmt=ahah

References

  1. Becker S. R., Kiessling N. L., Klein G. 1978; Modulation of sensitivity to natural killer cell lysis after in vivo explantation of a mouse lymphoma. Journal of the National Cancer Institute 61:1495–1498
    [Google Scholar]
  2. Bukowski J. F., Woda B. A., Habu S., Okumura K., Welsh R. M. 1983; Natural killer cell depletion enhances virus synthesis and virus-induced hepatitis in vivo. Journal of Immunology 131:1531–1537
    [Google Scholar]
  3. Bukowski J. F., Woda B. A., Welsh R. M. 1984; Pathogenesis of murine cytomegalovirus infection in natural killer cell-depleted mice. Journal of Virology 52:119–128
    [Google Scholar]
  4. Domok I., Magrath D. I. 1979; Guide to poliovirus isolation and serological techniques for poliomyelitis surveillance. WHO Offset Publication no 46:
    [Google Scholar]
  5. Fujii Y., Sendo F., Kamiyama T., Naiki M. 1990; IgG antibodies to asialo GM1 are more sensitive than IgM antibodies to kill in vivo natural killer cells and premature cytotoxic T lymphocytes of mouse spleen. Microbiology and Immunology 34:533–542
    [Google Scholar]
  6. Godeny E. K., Gauntt C. J. 1986; Involvement of natural killer cells in coxsackievirus B3-induced murine myocarditis. Journal of Immunology 137:1695–1702
    [Google Scholar]
  7. Godeny E. K., Gauntt C. J. 1987; Murine natural killer cells limit coxsackievirus B3 replication. Journal of Immunology 139:913–918
    [Google Scholar]
  8. Gronberg A., Ferm M. T., Craig J. N. G., Reynolds W., Ort aldo J. R. 1988; IFN-γ treatment of k562 cells inhibits natural killer cell triggering and decreases the susceptibility to lysis by cytoplasmic granules from large granular lymphocytes. Journal of Immunology 140:4397–4402
    [Google Scholar]
  9. Habu S., Fukui H., Shimamura K., Kasai M., Nagai Y., Okumura K., Tamaoki N. 1981; In vivo effects of anti-asialo GM 1.1. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice. Journal of Immunology 127:34–38
    [Google Scholar]
  10. Harel-Bellan A., Quillet C., Marchiol C., De Mars R., Tursz T., Fradelizi D. 1986; Natural killer susceptibility of human cells may be regulated by genes in the HL A region on chromosome 6. Proceedings of the National Academy of Sciences, U.S.A 83:5688–5692
    [Google Scholar]
  11. Karre K., Ljunggren H., Pionetek G., Kiessling R. 1986; Selective rejection of H-2-deficient lymphoma variants suggests alternative immune defence strategy. Nature, London 319:675–678
    [Google Scholar]
  12. Kasai M., Iwarnori M., Nagai Y., Okumura K., Tada T. 1980; A glycolipid on the surface of mouse natural killer cells. European Journal of Immunology 10:175–180
    [Google Scholar]
  13. Lanier L. L., Phillips J. H., Tutt M., Kumar V. 1986; Natural killer cells: definition of a cell type rather than a function. Journal of Immunology 137:2735–2739
    [Google Scholar]
  14. Ljunggren H., Karre K. 1985; Host resistance directed selectively against H-2-deficient lymphoma variants. Analysis of the mechanism. Journal of Experimental Medicine 162:1745–1759
    [Google Scholar]
  15. Ljunggren H., Ohlen C., Hoglund P., Yamaski T., Klein G., Karre K. 1988; Afferent and efferent cellular interactions in natural resistance directed against MHC class I deficient tumor grafts. Journal of Immunology 140:671–678
    [Google Scholar]
  16. Loria R. M., Montgomery L. B., Corey S. A., Chinchilli V. 1984; Coxsackievirus B4 infection in animals with diabetes melitus genotype. Archives of Virology 81:251–262
    [Google Scholar]
  17. Meynell G. G., Meynell E. (editors) 1970; Quantitative aspects of microbiological experiments. In Theory and Practice in Experimental Bacteriology 2nd edn pp 207–208 Cambridge: Cambridge University Press;
    [Google Scholar]
  18. Piontek G. E., Taniguchi K., Liunggren H., Gronberg A., Kiessling R., Klein G., Karre K. 1985; YAC-1 MHC class I variants reveal an association between decreased NK sensitivity and increased H-2 expression after interferon treatment or in vivo passage. Journal of Immunology 135:4281–4288
    [Google Scholar]
  19. Stitz L., Baenziger J., Pircher H., Hengartner H., Zinkernagel R. M. 1986; Effect of rabbit anti-asialo GM1 treatment in vivo or with anti-asialo GM1 plus complement in vitro on cytotoxic T cell activities. Journal of Immunology 136:4674–4680
    [Google Scholar]
  20. Storkus W. J., Howell D. N., Salter R. D., Dawson J. R., Cresswell P. 1987; NK susceptibility varies inversely with target cell class 1 HLA antigen expression. Journal of Immunology 138:1657–1659
    [Google Scholar]
  21. Szopa T. M., Dronfield D. M., Ward T., Taylor K. W. 1989; In vivo infection with coxsackie B4 virus induces long term functional changes in pancreatic islets with minimal alteration in blood glucose. Diabetic Medicine 6:314–319
    [Google Scholar]
  22. Toniolo A., Federico G., Basolo F., Onodera T. 1988; Diabetes mellitus. In Coxsackie Viruses - A General Update pp 353–360 Edited by Bendinelli M., Friedman H. New York: Plenum Press;
    [Google Scholar]
  23. Tornqvist L., Vartia P., Vartia Y. O. 1985; How should relative changes be measured?. American Statistics 39:43–46
    [Google Scholar]
  24. Vella C., Easton A. J., Eglin R. P., Brown C. L., Perry L. 1991; Coxsackievirus B4 infection of the mouse pancreas. 1. Detection of virus specific RNA in the pancreas by in situ hybridisation. Journal of Medical Virology 25:46–49
    [Google Scholar]
  25. Vella C., Brown C. L., McCarthy D. A. 1992; Coxsackievirus B4 infection of the mouse pancreas: acute and persistent infection. Journal of General Virology 73:1387–1394
    [Google Scholar]
  26. Welsh R. M. 1986; Regulation of virus infections by natural killer cells. A review. Natural Immunity and Cell Growth Regulation 5:169–199
    [Google Scholar]
  27. White L. L., Smith R. A. 1990; D variant of encephalomyocarditis virus (EMCV-D)-induced diabetes following natural killer cell depletion in diabetes-resistant male C57BL/6J mice. Viral Immunology 3:67–76
    [Google Scholar]
  28. Wolfgram L. J., Beisel K. W., Herskowitz A., Rose N. R. 1986; Variations in the susceptibility to coxsackie virus B3-induced myocarditis among different strains of mice. Journal of Immunology 136:1846–1852
    [Google Scholar]
  29. Woodruff J. F. 1979; Lack of correlation between neutralising antibody production and suppression of coxsackie virus B-3 replication in target organs: evidence for the involvement of mononuclear inflammatory cells in host defence. Journal of Immunology 123:31–36
    [Google Scholar]
  30. Woodruff J. F., Woodruff J. J. 1974; Involvement of T lymphocytes in the pathogenesis of coxsackievirus B3 heart disease. Journal of Immunology 113:1726–1734
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-6-1379
Loading
/content/journal/jgv/10.1099/0022-1317-73-6-1379
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error