1887

Abstract

Ultraviolet light-inactivated, non-infectious influenza virus is pyrogenic; virion components are probably responsible for this pyrogenicity. To try to identify the pyrogenic component, influenza virions were disrupted with either bromelain or sodium deoxycholate (DOC). Treatment of infectious virions with bromelain, under conditions that removed the surface glycoproteins (spikes), destroyed their pyrogenicity. The supernatant, containing non-aggregated and modified glycoproteins, was also non-pyrogenic. Disruption of virions with DOC considerably reduced pyrogenicity; however, some was retained by the sub-viral cores. Viral nucleoprotein and matrix protein, purified from the supernatant, were non-pyrogenic. Aggregated stellate clusters of surface glycoproteins separated on sucrose gradients were pyrogenic in half of numerous tests performed with different batches of material. Treatment of virus with ether resulted in complete loss of pyrogenicity. Liposomes made from extracted viral lipid were non-pyrogenic. In contrast, virosomes made from the viral lipid and the aggregated stellate clusters of surface glycoproteins were pyrogenic. Hence, optimum pyrogenicity depends upon the integrity of the virus particle, but haemagglutinin and/or neuraminidase appear essential, and lipid may be involved.

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1992-06-01
2024-03-29
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References

  1. Almeida J. D., Brand C. M., Edward D. C., Heath T. D. 1975; Formations of virosomes from influenza subunits and liposomes. Lancet ii:899–901
    [Google Scholar]
  2. Atkins E., Bodel P. 1974; Fever. In The Inflammatory Process pp 467–514 Edited by Zweibach B. W., Grant L., McClusky R. T. New York: Academic Press;
    [Google Scholar]
  3. Brand C. M., Liew F. Y. 1983; The estimation of host antigen in experimental and commercial influenza subunit preparations by delayed-type hypersensitivity reactions. Journal of Biological Standardization 11:313–321
    [Google Scholar]
  4. Brand C. M., Skehel J. J. 1972; Crystalline antigen from influenza virus envelope. Nature, London 233:145–149
    [Google Scholar]
  5. Chomik M. 1988a; Ultrastructure and some biological properties of influenza A virus. II. Effect of physico-chemical factors on influenza A virus and its interferogenic properties. Archivum Immunologiae et Therapiae Experimentalis 36:459–464
    [Google Scholar]
  6. Chomik M. 1988b; Ultrastructure and some biological properties of influenza A virus. III. The role of influenza A virus lipids in interferon induction. Archinum Immunologiae et Therapiae Experimentalis 36:547–554
    [Google Scholar]
  7. Chomik M., Slusarczyk A. 1988; Ultrastructure and some biological properties of influenza A virus. IV. Effect of proteolytic enzymes on viral particle. Archivum Immunologiae et Therapiae Experimentalis 36:555–566
    [Google Scholar]
  8. Coates D. M., Sweet C., Smith H. 1986; Severity of fever in influenza: differential pyrogenicity in ferrets exhibited by H1N1 and H3N2 strains of differing virulence. Journal of General Virology 67:419–425
    [Google Scholar]
  9. Dinarello C. A., Wolff S. M. 1982; Exogenous pyrogens. In Pyretics and Antipyretics pp 73–112 Edited by Milton A. S. New York: Springer-Verlag;
    [Google Scholar]
  10. Folch I., Lees M., Sloan-Stanley S. M. 1957; A simple method for the isolation and purification of total lipid from animal tissues. Journal of Biological Chemistry 226:497–500
    [Google Scholar]
  11. Frank A. L., Taber L. H., Wells J. M. 1985; Comparison of infection rates and severity of illness for influenza A subtypes H1N1 and H3N2. Journal of Infectious Diseases 151:73–80
    [Google Scholar]
  12. Gander G. W. 1982; Endogenous pyrogens. In Pyretics and Antipyretics pp 113–123 Edited by Milton A. S. New York: Springer-Verlag;
    [Google Scholar]
  13. Gregoriadis G., Leathwood P. D., Ryman B. E. 1971; Enzyme entrapment in liposomes. FEBS Letters 14:95–99
    [Google Scholar]
  14. Houde M., Arora D. J. S. 1989; Human influenza virus neuraminidase, but not haemagglutinin, induces mouse macrophage interleukin-1 in vivo and in vitro. Immunology Letters 22:41–46
    [Google Scholar]
  15. Houde M., Arora D. J. S. 1990; Stimulation of tumor necrosis factor secretion by purified influenza virus neuraminidase. Cellular Immunology 29:104–111
    [Google Scholar]
  16. Jakeman K. J., Bird C. R., Thorpe R., Smith H., Sweet C. 1991; Nature of the endogenous pyrogen (EP) induced by influenza viruses: lack of correlation between EP levels and content of known pyrogenic cytokines, interleukin 1, interleukin 6 and tumour necrosis factor. Journal of General Virology 72:705–709
    [Google Scholar]
  17. Kanoh S., Kawasaki H. 1966; Studies on the myxovirus pyrogen. I. Interaction of myxovirus and rabbit polymorphonuclear leucocytes. Biken Journal 9:177–184
    [Google Scholar]
  18. Kung H. C., Jen K. F., Yuan W. C., Tien S. F., Chu C. M. 1978; Influenza in China in 1977, recurrence of influenza virus A subtype H1N1. Bulletin of the World Health Organization 56:913–918
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of a bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Laver W. G., Valentine R. C. 1969; Morphology of the isolated hemagglutinin and neuraminidase subunits of influenza virus. Virology 38:105–119
    [Google Scholar]
  21. Liew F. Y., Russell S. M., Brand C. M. 1979; Identification and characterization of delayed type hypersensitivity to influenza virus in mice. European Journal of Immunology 9:783–790
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Majde J. A., Brown R. K., Jones M. W., Dieffenbach C. W., Maitra N., Krueger J. M., Cady A. S., Smitka C. W., Maassab H. F. 1991; Detection of toxic viral-associated double stranded RNA (dsRNA) in influenza-infected lung. Microbial Pathogenesis 10:105–115
    [Google Scholar]
  24. Matsuyama T., Sweet C., Collie M. H., Smith H. 1980; Aspects of virulence in ferrets exhibited by influenza virus recombinants of known genetic constitution. Journal of Infectious Diseases 141:351–361
    [Google Scholar]
  25. Rawlins M. D., Cranston W. I. 1973; Clinical studies on the pathogenesis of fever. In The Pharmacology of Thermoregulation pp 264–277 Edited by Schonbaum E., Lomax P. Basel: S. Karger;
    [Google Scholar]
  26. Scott G. M., Secher D. S., Flowers D., Bate J., Cantell K., Tyrrell D. A. J. 1981; Toxicity of interferon. British Medical Journal 282:1345–1348
    [Google Scholar]
  27. Siegert R., Braune P. 1963; The pyrogens of myxoviruses: resistance of influenza A pyrogen to heat, ultraviolet and chemical treatment. Virology 24:218–224
    [Google Scholar]
  28. Sweet C., Bird R. A., Cavanagh D., Toms G. L., Collie M. H., Smith H. 1979; The local origin of the febrile response induced in ferrets during respiratory infection with a virulent influenza virus. British Journal of Experimental Pathology 60:300–308
    [Google Scholar]
  29. Sweet C., Stephen J., Smith H. 1974a; Purification of influenza viruses using disulphide-linked immunosorbents derived from rabbit antibody. Immunochemistry 11:295–304
    [Google Scholar]
  30. Sweet C., Stephen J., Smith H. 1974b; The behaviour of antigenically related influenza viruses of differing virulence on disulphide-linked immunosorbents. Immunochemistry 11:823–826
    [Google Scholar]
  31. Sweet C., Stephen J., Smith H. 1974c; Immunization of ferrets against influenza: a comparison of killed ferret grown and egg grown virus. British Journal of Experimental Pathology 55:296–304
    [Google Scholar]
  32. Tinsley C. M., Sweet C., Coates D. M., Smith H. 1987; The local origin of fever in influenza: differential production of endogenous pyrogen by nasal inflammatory cells of ferrets exhibiting different levels of fever. FEMS Microbiology Letters 42:103–108
    [Google Scholar]
  33. Toms G. L., Davies J. A., Woodward C. G., Sweet C., Smith H. 1977; The relation of pyrexia and nasal inflammatory response to virus levels in nasal washings of ferrets infected with influenza viruses of differing virulence. British Journal of Experimental Pathology 58:444–449
    [Google Scholar]
  34. Tyler K. L., Fields B. N. 1985; Reovirus and its replication. In Virology pp 817–862 Edited by Fields B. N., Knipe D. M., Chunock R. M., Melnick J. L., Roizman B., Shope R. E. New York: Raven Press;
    [Google Scholar]
  35. Wagner R. R., Bennett I. L., Lequine V. S. 1949; The production of fever by influenza viruses. I. Factors influencing the febrile response to single infections of virus. Journal of Experimental Medicine 90:321–334
    [Google Scholar]
  36. Wright P. F., Thompson J., Karzon D. T. 1981; Differing virulence of Hl N1 and H3N2 influenza strains. American Journal of Epidemiology 112:814–819
    [Google Scholar]
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