1887

Abstract

The complete nucleotide sequence of RNA 4 of rice stripe virus isolate T (RSV-T) was determined and found to consist of 2157 nucleotides, containing two open reading frames (ORFs). One, deduced to be present in the 5′-proximal region of the viral-sense RNA, encodes the stripe disease-specific protein with 20541, and the other ORF, in the 5′-proximal region of the viral complementary sense RNA, encodes an unknown protein with 32474. Between these two ORFs there is an intergenic non-coding region that could form a secondary structure with two base-paired hairpin configurations. These characteristics indicate that RSV-T RNA 4 has an ambisense coding strategy. Comparison of the two ORFs of RSV-T with those of another isolate revealed 97.2% and 98.0% identity for the nucleotide sequences, and 98.3% and 98.2% identity for the amino acid sequences. The leader sequences of these two isolates were the same. However, an insertion was found in the intergenic non-coding region of RSV-T. Furthermore, comparison of the nucleotide and amino acid sequences of RSV-T RNA 4 with those of RNA 4 of maize stripe virus, which is another member of the tenuivirus group, revealed greater identity, suggesting a close phylogenetic relationship between these two viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-5-1309
1992-05-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/5/JV0730051309.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-5-1309&mimeType=html&fmt=ahah

References

  1. Aupbrin D. D., Romanowski V., Galinski M., Bishop D. H. L. 1984; Sequencing studies of Pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA. Journal of Virology 51:897–904
    [Google Scholar]
  2. Brow M. A. D. 1990; Sequencing with Taq DNA polymerase. In PCR Protocols. A Guide to Methods and Applications pp 189–196 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. New York & London: Academic Press;
    [Google Scholar]
  3. de Haan P., Wagemakers L., Peters D., Goldbach R. 1990; The S RNA segment of tomato spotted wilt virus has an ambisense character. Journal of General Virology 71:1001–1007
    [Google Scholar]
  4. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991 Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses pp 398–399 Wien & New York: Springer-Verlag;
    [Google Scholar]
  5. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  6. Hanahan D. 1985; Techniques for transformation of E. coli. In DNA Cloning: A Practical Approach vol 1 pp 109–135 Edited by Glover D. M. Oxford: IRL Press;
    [Google Scholar]
  7. Hayano Y., Kakutani T., Hayashi T., Minobe Y. 1990; Coding strategy of rice stripe virus: major nonstructural protein is encoded in viral RNA segment 4 and coat protein in RNA complementary to segment 3. Virology 177:372–374
    [Google Scholar]
  8. Hayashi T., Usugi T., Nakano M., Ishikawa K. 1990; On the strains of rice stripe virus. (1) An attempt to detect strains by differences in molecular size of disease-specific proteins. Proceedings of the Association for Plant Protection of Kyushu 35:1–2
    [Google Scholar]
  9. Huiet L., Klaassen V., Tsai J. H., Falk B. W. 1990; Identification and sequence analysis of the maize stripe virus major noncapsid protein gene. Virology 179:862–866
    [Google Scholar]
  10. Huiet L., Klaassen V., Tsai J. H., Falk B. W. 1991; Nucleotide sequence and RNA hybridization analysis reveal an ambisense coding strategy for maize stripe virus RNA3. Virology 181:47–53
    [Google Scholar]
  11. Ishikawa K., Omura T., Tsuchizaki T. 1989; Association of double- and single-stranded RNAs with each of the four components of rice stripe virus. Annals of the Phytopathological Society of Japan 55:315–323
    [Google Scholar]
  12. Kakutani T., Hayano Y., Hayashi T., Minobe Y. 1990; Ambisense segment 4 of rice stripe virus: possible evolutionary relationship with phleboviruses and uukuviruses (Bunyaviridae). Journal of General Virology 71:1427–1432
    [Google Scholar]
  13. Kakutani T., Kayano Y., Hayashi T., Minobe Y. 1991; Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments. Journal of General Virology 72:465–468
    [Google Scholar]
  14. Kiso A., Yamamoto T. 1973; Infection and symptom development in rice stripe disease, with special reference to disease-specific protein other than virus. Review of Plant Protection Research 6:75–100
    [Google Scholar]
  15. Koganezawa H., Doi Y., Yora K. 1975; Purification of rice stripe virus. Annals of the Phytopathological Society of Japan 41:148–154
    [Google Scholar]
  16. Maiss E., Ivanova L., Breyel E., Adam G. 1991; Cloning and sequencing of the S RNA from a Bulgarian isolate of tomato spotted wilt virus. Journal of General Virology 72:461–464
    [Google Scholar]
  17. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 139:487–491
    [Google Scholar]
  18. Takahashi M., Toriyama S., Kikuchi Y., Hayakawa T., Ishihama A. 1990; Complementarity between the 5′- and 3′-terminal sequences of rice stripe virus RNAs. Journal of General Virology 71:2817–2821
    [Google Scholar]
  19. Tinoco J. I., Borer P. N., Dengler B., Levine M. D., Uhlenbeck O. C., Crothers D. W., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature New Biology 246:40–41
    [Google Scholar]
  20. Toriyama S. 1982; Characterization of rice stripe virus: a heavy component carrying infectivity. Journal of General Virology 61:187–195
    [Google Scholar]
  21. Toriyama S. 1986; Rice stripe virus: prototype of a new group of viruses that replicate in plants and insects. Microbiological Sciences 3:347–351
    [Google Scholar]
  22. Toriyama S., Watanabe Y. 1989; Characterization of single- and double-stranded RNAs in particles of rice stripe virus. Journal of General Virology 70:505–511
    [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  24. Zhu Y., Hayakawa T., Toriyama T., Takahashi M. 1991; Complete nucleotide sequence of RNA 3 of rice stripe virus: an ambisense coding strategy. Journal of General Virology 72:763–767
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-73-5-1309
Loading
/content/journal/jgv/10.1099/0022-1317-73-5-1309
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error