1887

Abstract

The incubation of vaccinia virus cores in appropriate conditions promotes the release of core proteins into a supernatant fraction. Under transcription assay conditions core mRNAs are extruded in association with viral core proteins, however the presence of these proteins within the core particle is not essential for RNA synthesis and extrusion. The RNA-protein complex is resistant to micrococcal nuclease. Five proteins of 60K, 43K, 28K, 18K and 14.5K with RNA-binding abilities have been identified by [P]RNA overlay protein blot assays. These proteins are likely to be a component of the viral ribonucleoprotein complex since core basic proteins with similar s have been identified and at least one RNA-binding protein is predicted in the vaccinia virus genome.

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1992-05-01
2021-10-19
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References

  1. Adam S. A., Choi Y. D., Dreyfuss G. 1986; The interaction of mRNA with proteins in VSV infected cells. Journal of Virology 57:614–622
    [Google Scholar]
  2. Bablanian R. 1984; Poxvirus cytopathogenicity: effects on cellular macromolecular synthesis. In Comprehensive Virology vol 19 pp 391–427 Edited by Wagner R. R., Fraenkel-Conrat H. New York: Plenum Press;
    [Google Scholar]
  3. Ben-Hamida F., Person A., Beaud G. 1983; Solubilization of a protein synthesis inhibitor from vaccinia virions. Journal of Virology 45:452–455
    [Google Scholar]
  4. Boone R. F., Moss B. 1978; Sequence complexity and relative abundance of vaccinia virus mRNAs synthesized in vivo and in vitro. Journal of Virology 26:554–569
    [Google Scholar]
  5. Bowen B., Steinberg J., Laemmli U. K., Weintraub H. 1980; The detection of DNA-binding proteins by protein blotting. Nucleic Acids Research 8:1–20
    [Google Scholar]
  6. Cabrera C. V., Esteban M., McCarron R., McAllister W. T., Holowczak J. 1978; Vaccinia virus transcription: hybridization of mRNA to restriction fragments of vaccinia DNA. Virology 86:102–114
    [Google Scholar]
  7. Cooper J. A., Moss B. 1978; Transcription of vaccinia virus mRNA coupled to translation in vitro. Virology 88:149–165
    [Google Scholar]
  8. Dâmaso C. R. A., Moussatché N. 1992; Proteins released from vaccinia cores are probably not involved in protein synthesis inhibition in vitro. Brazilian Journal of Medical and Biological Research (in press)
    [Google Scholar]
  9. Dreyfuss G. 1986; Structure and function of nuclear and cytoplasmic ribonucleoprotein particles. Annual Review of Cell Biology 2459–498
    [Google Scholar]
  10. Dynan W. S., Burgess R. R. 1979; In vitro transcription by wheat germ ribonucleic acid polymerase II. Effect of heparin and the role of templete integrity. Biochemistry 18:4581–4588
    [Google Scholar]
  11. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  12. Greenberg J. 1977; Isolation of messenger ribonucleoproteins in cesium sulfate density gradient: evidence that polyadenylate and non-polyadenylate messenger RNAs are associated with protein. Journal of Molecular Biology 108:403–416
    [Google Scholar]
  13. Ichihashi Y., Oie M., Tsuruhara T. 1984; Location of DNA-binding proteins and disulfide-linked proteins in vaccinia virus structural elements. Journal of Virology 50:929–938
    [Google Scholar]
  14. Kao S.-Y., Ressner E., Kates J., Bauer W. R. 1981; Purification and characterization of a superhelix binding protein from vaccinia virus. Virology 111:500–508
    [Google Scholar]
  15. Kates J. R., Beeson J. 1970; Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores. Journal of Molecular Biology 50:1–18
    [Google Scholar]
  16. Kates J. R., McAuslan B. R. 1967; Poxvirus DNA-dependent RNA polymerase. Proceedings of the National Academy of Sciences, U.S.A. 58:134–141
    [Google Scholar]
  17. Konarska M. M. 1989; Analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis. Methods in Enzymology 180:422–453
    [Google Scholar]
  18. Kumar A., Pederson T. 1975; Comparison of proteins bound to heterogeneous nuclear RNA and messenger RNA in HeLa cells. Journal of Molecular Biology 9:353–365
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Metz D. H., Esteban M., Danielescu G. 1975; The formation of virus polyribosomes in L cells infected with vaccinia virus. Journal of General Virology 27:181–195
    [Google Scholar]
  21. Morel C., Kayibanda B., Scherrer K. 1971; Proteins associated with globin messenger RNA in avian erythroblasts: isolation and comparison with proteins bound to nuclear messenger-like RNA. FEBS Letters 18:8488
    [Google Scholar]
  22. Moss B. 1990; Poxviridae and their replication. In Virology 2nd edn., pp 2079–2111 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  23. Moussatché N. 1985; Polyamines stimulate DNA-dependent RNA synthesis catalysed by vaccinia virus. Biochimica et biophysica acta 826:113–120
    [Google Scholar]
  24. Moussatché N., Keller S. J. 1991; Phosphorylation of vaccinia virus proteins during transcription in vitro. Journal of Virology 65:2555–2561
    [Google Scholar]
  25. Munyon W. E., Paoletti E., Grace J. T. Jr 1967; RNA polymerase activity in purified infectious vaccinia virus. Proceedings of the National Academy of Sciences, U.S.A. 58:2280–2288
    [Google Scholar]
  26. Oie M., Ichihashi Y. 1981; Characterization of vaccinia polypeptides. Virology 113:263–276
    [Google Scholar]
  27. Paoletti E. 1977; In vitro synthesis of a high molecular weight virion-associated RNA by vaccinia. Journal of Biological Chemistry 252:856–861
    [Google Scholar]
  28. Pelham H. B., Sykes J. M. M., Hunt T. 1978; Characteristics of a coupled cell-free transcription and translation system directed by vaccinia cores. European Journal of Biochemistry 82:199–209
    [Google Scholar]
  29. Richter J. D., Smith L. D. 1983; Developmentally regulated RNA binding proteins during oogenesis in Xenopus laevis. Journal of Biological Chemistry 258:4864–4869
    [Google Scholar]
  30. Rosen C. A., Siekierka J., Ennis H. L., Cohen P. S. 1984; Inhibition of protein synthesis in vesicular stomatitis virus infected Chinese hamster ovary cells: role of virus mRNA-ribonucleoprotein particle. Biochemistry 23:2407–2411
    [Google Scholar]
  31. Rosen C. A., Sodroski J. G., Chun-Goh W. C., Dayton A. I., Lippke J., Haseltine W. A. 1986; Post-transcriptional regulation accounts for the transactivation of the human T-lymphotropic virus type III. Nature, London 319:555–559
    [Google Scholar]
  32. Sagot J., Beaud G. 1979; Phosphorylation in vivo of a vaccinia virus structural protein found associated with the ribosomes from infected cells. European Journal of Biochemistry 98:131–140
    [Google Scholar]
  33. Sarov I., Joklik W. K. 1972; Studies on the nature of the capsid polypeptides of vaccinia virions. Virology 50:579–592
    [Google Scholar]
  34. Smith G. S., Chan Y. S., Howard S. T. 1991; Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat. Journal of General Virology 72:1349–1376
    [Google Scholar]
  35. Soloski M. J., Esteban M., Holowczak J. A. 1978; DNA binding proteins in the cytoplasm of vaccinia virus-infected mouse L cells. Journal of Virology 25:263–273
    [Google Scholar]
  36. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  37. Van Eekelen C. A. G., Mariman E. C. M., Reinders R. J., van Venroou W. J. 1981; Adenoviral heterogeneous nuclear RNA is associated with host cell proteins. European Journal of Biochemistry 119:461–473
    [Google Scholar]
  38. Yang W.-P., Bauer W. R. 1988; Purification and characterization of vaccinia virus structural protein VP8. Virology 167:578–584
    [Google Scholar]
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