1887

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Volume 73, Issue 5

Identification of the equine herpesvirus type 1 glycoprotein 17/18 as a homologue of herpes simplex virus glycoprotein D Free

Abstract

The DNA sequence of the equine herpesvirus type 1 (EHV-1) gD gene homologue has been determined for the strain Ab1 and compared with previously published sequences. A portion of the gene has been located to a region of the genome which also encodes homologues of the herpes simplex virus type 1 genes for gE and gI and is known to encode an epitope of the virion protein gp17/18. Analysis of the EHV-1 strain Kentucky A (KyA) by DNA hybridization showed the presence of a gD gene homologue and established the absence of genes for gI and gE. Western blot analysis, however, showed that KyA virus particles contain gp17/18, thus indicating that this protein is encoded by the gD gene homologue. The KyA gp17/18 was found to be smaller than that detected in other strains and this is accounted for by a frameshift mutation in the KyA sequence relative to Ab1. The mutation in the KyA strain results in an altered C-terminal sequence and could explain the apparent structural differences suggested by the reactivities with monoclonal antibodies (MAbs). We have also expressed part of the Ab1 gD gene as a fusion protein with glutathione -transferase in and shown that this reacts with the MAb 5H6 originally used to map gp17/18. These experiments establish that gp17/18 is encoded by the gD gene homologue.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
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1992-05-01
2024-04-19
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References

  1. Adler R., Glorioso J. C., Cossman J., Levine M. 1978; Possible role of Fc receptors on cells infected and transformed by herpesvirus: escape from immune cytolysis. Infection and Immunity 21:442–447
     [Google Scholar]
  2. Allen G. P., Coogle L. D. 1988; Characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gpl3) with homology to herpes simplex virus glycoprotein C. Journal of Virology 62:2850–2858
     [Google Scholar]
  3. Allen G. P., Yeargan M. R. 1987; Use of λgt 11 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus 1. Journal of Virology 61:2454–2461
     [Google Scholar]
  4. Audonnet J. -C., Winslow J., Allen G., Paoletti E. 1990; Equine herpesvirus type 1 unique short fragment encodes glycoproteins with homology to herpes simplex virus type 1 gD, gI and gE. Journal of General Virology 71:2969–2978
     [Google Scholar]
  5. Bell S., Cranage M., Borysiewicz L., Minson T. 1990; Induction of immunoglobulin G Fc receptors by recombinant vaccinia viruses expressing glycoproteins E and I of herpes simplex virus type 1. Journal of Virology 64:2181–2186
     [Google Scholar]
  6. Davison A. J. 1983; DNA sequence of the US component of the varicella-zoster virus genome. EMBO Journal 2:2203–2209
     [Google Scholar]
  7. Elton D. M., Bonass W. A., Killington R. A., Meredith D. M., Halliburton I. W. 1991a; Location of open reading frames coding for equine herpesvirus type-1 glycoproteins with homology to gE and gI of herpes simplex virus. American Journal of Veterinary Research 52:1252–1257
     [Google Scholar]
  8. Elton D. M., Halliburton I. W., Killington R. A., Meredith D. M., Bonass W. A. 1991b; Sequence analysis of the 4·7 kb Bam HI-Eco RI fragment of the equine herpesvirus type-1 short unique region. Gene 101:203–208
     [Google Scholar]
  9. Flowers C. C., Eastman E. M., O’Callaghan D. J. 1991; Sequence analysis of a glycoprotein D gene homolog within the unique short segment of the EHV-1 genome. Virology 180:175–184
     [Google Scholar]
  10. Frame M. C., Marsden H. S., McGeoch D. J. 1986; Novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene US4. Journal of General Virology 67:745–751
     [Google Scholar]
  11. Frink R. J., Eisenberg R., Cohen G., Wagner E. K. 1983; Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein C. Journal of Virology 45:634–647
     [Google Scholar]
  12. Gompels U., Minson A. 1986; The properties and sequence of glycoprotein H of herpes simplex virus type 1. Virology 153:230–247
     [Google Scholar]
  13. Hanke T., Graham F. L., Lulitanond V., Johnson D. C. 1990; Herpes simplex virus IgG Fc receptors induced using recombinant adenovirus vectors expressing glycoproteins E and I. Virology 177:437–444
     [Google Scholar]
  14. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
     [Google Scholar]
  15. Henry B. E., Robinson R. A., Dauenhauer S. A., Atherton S. S., Hayward G. S., O’Callaghan D. J. 1981; Structure of the genome of equine herpesvirus type 1. Virology 115:97–114
     [Google Scholar]
  16. Highlander S. L., Cai W., Person S., Levine M., Glorioso J. C. 1988; Monoclonal antibodies define a domain on herpes simplex virus glycoprotein B involved in virus penetration. Journal of Virology 62:1881–1888
     [Google Scholar]
  17. Keller P. M., Davison A. J., Lowe R. S., Bennett C. D., Ellis R. W. 1986; Identification and structure of the gene encoding gpII, a major glycoprotein of varicella-zoster virus. Virology 152:181–191
     [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembley of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  19. Longnecker R., Chatterjee S., Whitley R. J., Roizman B. 1987; Identification of a herpes simplex virus 1 glycoprotein gene within a gene cluster dispensable for growth in cell culture. Proceedings of the National Academy of Sciences, U.S.A. 84:4303–4307
     [Google Scholar]
  20. McGeoch D. J., Davison A. J. 1986; DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus. Nucleic Acids Research 14:4281–4292
     [Google Scholar]
  21. McGeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
     [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  23. Meredith D. M., Stocks J. M., Whittaker G. R., Halliburton I. W., Snowden B. W., Killington R. A. 1989; Identification of the gB homologues of equine herpesvirus types 1 and 4 as disulphide-linked heterodimers and their characterization using monoclonal antibodies. Journal of General Virology 70:1161–1172
     [Google Scholar]
  24. Perdue M. L., Kemp M. C., Randall C. C., O’Callaghan D. J. 1974; Studies of the molecular anatomy of the L-M cell strain of equine herpes virus type 1: proteins of the nucleocapsid and intact virion. Virology 59:201–216
     [Google Scholar]
  25. Petrovskis E. A., Timmins J. G., Armentrout M. A., Marchioli C. C., Yancey R. J. Jr, Post L. E. 1986a; DNA sequence of the gene for pseudorabies virus gp50, a glycoprotein without W-linked glycosylation. Journal of Virology 59:216–223
     [Google Scholar]
  26. Petrovskis E. A., Timmins J. G., Post L. E. 1986b; Use of λgt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins. Journal of Virology 60:185–193
     [Google Scholar]
  27. Rea T. J., Timmins J. G., Long G. W., Post L. E. 1985; Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells. Journal of Virology 54:21–29
     [Google Scholar]
  28. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
     [Google Scholar]
  29. Robbins A. K., Dorney D. J., Wathen M. W., Whealy M. E., Gold C., Watson R. J., Holland L. E., Weed S. D., Levine M., Glorioso J. C., Enquist L. W. 1987; The psuedorabies virus gII gene is closely related to the gB glycoprotein gene of herpes simplex virus. Journal of Virology 61:2691–2701
     [Google Scholar]
  30. Robbins A. K., Whealy M. E., Enquist L. W. 1988; Centrifugation procedures for studying herpesviruses using the Sorvall RC-28S Supraspeed centrifuge. Biotechnology Update (DuPont) 3:1–3
     [Google Scholar]
  31. Smith D. B., Johnson K. S. 1988; Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31–40
     [Google Scholar]
  32. Stokes A., Corteyn A. H., Pullen L. A., Doel T. R., Meredith D. M., Killington R. A., Halliburton I. W., Whittaker G. R., Wheldon L. A., Nicholson L., Onions D. E., Allen G. P., Murray P. K. 1991; Studies on glycoprotein 13 (gp13) of equid herpesvirus 1 using affinity-purified gp13, glycoprotein-specific monoclonal antibodies and synthetic peptides in a hamster model. Journal of General Virology 72:923–931
     [Google Scholar]
  33. Strynadka N. C. J., Redmond M. J., Parker J. M. R., Scraba D. G., Hodges R. S. 1988; Use of synthetic peptides to map the antigenic determinants of glycoprotein D of herpes simplex virus. Journal of Virology 61:3474–3483
     [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
     [Google Scholar]
  35. Whalley J. M., Robertson G. R., Davison A. J. 1981; Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases Eco RI, Bgl II and Bam HI. Journal of General Virology 57:307–323
     [Google Scholar]
  36. Whalley J. M., Robertson G. R., Scott N. A., Hudson G. C., Bell C. W., Woodworth L. M. 1989; Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB. Journal of General Virology 70:383–394
     [Google Scholar]
  37. Whittaker G. R., Taylor L. A., Elton D. M., Giles L. E., Bonass W. A., Halliburton I. W., Killington R. A., Meredith D. M. 1992; Glycoprotein 60 of equine herpesvirus type 1 is a homologue of herpes simplex virus glycoprotein D and plays a major role in the penetration of cells. Journal of General Virology 73:801–809
     [Google Scholar]
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Identification of the equine herpesvirus type 1 glycoprotein 17/18 as a homologue of herpes simplex virus glycoprotein D
J. Gen. Virol. 73, 1227 (1992); https://doi.org/10.1099/0022-1317-73-5-1227
/content/journal/jgv/10.1099/0022-1317-73-5-1227
/content/journal/jgv/10.1099/0022-1317-73-5-1227
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