Molecular cloning and physical mapping of the genome of simian herpes B virus and comparison of genome organization with that of herpes simplex virus type 1
The molecular structure of the genome of simian herpes B virus (SHBV) was determined by restriction endonuclease mapping studies. Genomic DNA was cleaved with restriction endonucleases BamHI and SalI into 41 and 58 fragments, respectively. Most of these fragments were cloned into the plasmid vector pACYC184; uncloned fragments were identified following isolation from agarose gels. Terminal fragments were identified by exonuclease digestion and radioactive end-labelling, and linkage of fragments was deduced by a combination of single and double digest experiments and cross-blot hybridizations. The genome is larger than that of herpes simplex virus type 1 (HSV-1), being approximately 165 kilobase pairs. Like that of HSV-1, the SHBV genome is composed of a long and a short unique region each flanked by inverted repeat sequences, which allow the unique regions to invert relative to one another, resulting in four possible isomeric arrangements of the molecule. Genome locations of several SHBV genes were compared with their HSV-1 homologues.
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. M.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.1987Current Trends in Molecular Biology vol 1 New York: John Wiley & Sons;
BoyerH. W.,
Roulland-DussoixD.1969; A complementation analysis of the restriction and modification of DNA in E. coli. Journal of Molecular Biology 41:459–472
BuckmasterA. E.,
ScottS. D.,
SandersonM. J.,
BoursnellM. E. G.,
RossL. J. N.,
BinnsM. M.1988; Gene sequence and mapping data from Marek’s disease virus and herpesvirus of turkeys: implications for herpesvirus classification. Journal of General Virology 69:2033–2042
ChangA. C. Y.,
CohenS. N.1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
DavisonA. J.,
McGeochD. J.1986; Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. Journal of General Virology 67:597–611
DavisonA. J.,
WilkieN. M.1983; Location and orientation of homologous sequences in the genomes of five herpesviruses. Journal of General Virology 64:1927–1942
FeinbergA. D.,
VogelsteinB.1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
GoldinA. L.,
Sandri-GoldinR. M.,
LevineM.,
GloriosoJ. C.1981; Cloning of herpes simplex virus type 1 sequences representing the whole genome. Journal of Virology 38:50–58
HilliardJ. K.,
MunozR. M.,
LipperS. L.,
EberleR.1986; Rapid identification of herpesvirus simiae (B virus) from clinical isolates in non-human primates. Journal of Virological Methods 13:55–62
HolmesG. P.,
HilliardJ. K.,
KlontzK. C.,
RupertA. H.,
SchindlerC. M.,
ParrishE.,
GriffinD. G.,
WardG. S.,
BernsteinN. D.,
BeanT. W.,
BallM. R.,
BradyJ. A.,
WilderM. H.,
KaplanJ. E.1990; B virus (herpesvirus simiae) infection in humans: epidemiologic investigation of a cluster. Annals of Internal Medicine 112:833–839
HonessR. W.,
CraxtonM. A.,
WilliamsL.,
GompelU. A.1989; A comparative analysis of the sequence of the thymidine kinase gene of a gammaherpesvirus, herpesvirus saimiri. Journal of General Virology 70:3003–3013
LudwigH.,
PauliG.,
GelderblomH.,
DaraiG.,
KochH. -G.,
FlugelR. M.,
NorrildB.,
DanielM. D.1983; B virus (herpesvirus simiae). In The Herpesviruses vol 2 pp 385–428 Edited by
RoizmanB.
New York: Plenum Press;
McGeochD. J.,
DolanA.,
DonaldS.,
RixonF. J.1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
McGeochD. J.,
DalyrmpleM. A.,
DavisonA. J.,
DolanA.,
FrameM. C.,
McNabD.,
PerryL. J.,
ScottJ. E.,
TaylorP.1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
McKnightS. L.,
GavisE. R.1980; Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro. Nucleic Acids Research 8:5931–5948
MittalS. K.,
FieldH. J.1989; Analysis of the bovine herpesvirus type 1 thymidine kinase (TK) gene from wild-type virus and TK- deficient mutants. Journal of General Virology 70:901–918
NicolsonL.,
CullinaneA. A.,
OnionsD. E.1990; The nucleotide sequence of the equine herpesvirus 4 thymidine kinase gene. Journal of General Virology 71:1801–1805
OrcuttR. P.,
PucakG. J.,
FosterH. L.,
KilcourseJ. T.,
FerrellT.1976; Multiple testing for the detection of B virus antibody in specially handled rhesus monkeys after capture from virgin trapping grounds. Laboratory Animal Science 26:70–74
RobertsonG. R.,
WhalleyJ. M.1988; Evolution of the herpes thymidine kinase gene: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell encoded thymidylate kinase. Nucleic Acids Research 16:11303–11317
RoizmanB.,
JacobR. J.,
KnifeD. M.,
MorseL. S.,
RuyechanW. T.1979; On the structure, functional equivalence and replication of the four arrangements of herpes simplex virus DNA. Cold Spring Harbor Symposia on Quantitative Biology 43:809–826
RossL. J. N.,
BinnsM. M.1991; Properties and evolutionary relationships of the Marek’s disease virus homologues of protein kinase, glycoprotein D and glycoprotein I of herpes simplex virus. Journal of General Virology 72:939–947
RossL. J. N.,
BinnsM. M.,
PastorekJ.1991; DNA sequence and organization of genes in a 5.5 kbp Eco RI fragment mapping in the short unique segment of Marek’s disease virus (strain RB1B). Journal of General Virology 72:949–954
SabinA. B.,
WrightW. M.1934; Acute ascending myelitis following a monkey bite, with the isolation of a virus capable of reproducing the disease. Journal of Experimental Medicine 59:115–136
ScottS. D.,
RossN. L. J.,
BinnsM. M.1989; Nucleotide and predicted amino acid sequences of the Marek’s disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses. Journal of General Virology 70:3055–3065
WallL. V. M.,
ZwartouwH. T.,
KellyD. C.1989; Discrimination between twenty isolates of herpesvirus simiae (B virus) by restriction enzyme analysis of the viral genome. Virus Research 12:283–296
WeststrateM. W.,
GeelenJ. L. M. C.,
van der NoordaaJ.1980; Human cytomegalovirus DNA: physical maps for the restriction endonucleases BglII, Hindlll and Xbal. Journal of General Virology 49:1–21
WilkieN. M.1976; Physical maps for herpes simplex virus type 1 DNA for restriction endonucleases HindIII, HpaI and X.bad. Journal of Virology 20:222–233
Molecular cloning and physical mapping of the genome of simian herpes B virus and comparison of genome organization with that of herpes simplex virus type 1