1887

Abstract

The nucleotide sequence of the P gene of the porcine paramyxovirus La-Piedad-Michoacan-Mexico virus (LPMV) was analysed. Three long open reading frames (ORFs) were found in the mRNA sense. Insertion of two G residues is necessary to obtain an ORF encoding the P protein, which gives a P protein of 404 amino acids with a calculated of 42475. This form of editing was demonstrated, two non-templated G residues being added in a portion of the mRNA transcripts. The LPMV V protein, which has a conserved cysteine-rich C-terminal region, is encoded by an exact copy of the P gene. The third ORF has the capacity to encode a protein of 126 amino acids, which may resemble the C proteins found in some paramyxoviruses. The ORF starts from an AUG codon down-stream of the first AUG codon of the P/V ORF.

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1992-05-01
2024-06-25
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References

  1. Berg M., Sundqvist A., Moreno-López J., Linné T. 1991; Identification of the porcine paramyxovirus LPMV matrix protein gene: comparative sequence analysis with other paramyxoviruses. Journal of General Virology 72:1045–1050
    [Google Scholar]
  2. Cattaneo R. 1991; Different types of messenger RNA editing. Annual Review of Genetics 25:71–88
    [Google Scholar]
  3. Cattaneo R., Kaelin K., Baczko K., Billeter M. A. 1989; Measles virus editing provides an additional cysteine-rich protein. Cell 56:759–764
    [Google Scholar]
  4. Chattopadhyay D., Banerjee A. K. 1987; Phosphorylation within a specific domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro. Cell 49:407–414
    [Google Scholar]
  5. Curran J., Kolakofsky D. 1988; Scanning-independent ribosomal initiation of the Sendai virus X protein. EMBO Journal 7:2869–2874
    [Google Scholar]
  6. Curran J., de Melo M., Moyer S., Kolakofsky D. 1991; Characterization of the Sendai virus V protein with an antipeptide antiserum. Virology 184:108–116
    [Google Scholar]
  7. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  8. Elliott G. D., Yeo R. P., Afzal M. A., Simpson E. J. B., Curran J. A., Rima B. K. 1990; Strain-variable editing during transcription of the P gene of mumps virus may lead to the generation of non-structural proteins NS1 (V) and NS2. Journal of General Virology 71:1555–1560
    [Google Scholar]
  9. Galinski M. S., Mink M. A., Lambert D. M., Wechsler S. L., Pons M. W. 1986; Molecular cloning and sequence analysis of the human parainfluenza 3 virus mRNA encoding the P and C proteins. Virology 155:46–60
    [Google Scholar]
  10. Hamaguchi M., Yoshida T., Nishikawa K., Naruse H., Nagai Y. 1983; Transcriptive complex of Newcastle disease virus. Virology 128:105–117
    [Google Scholar]
  11. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  12. Klug A., Rhodes D. 1987; ‘Zinc fingers’: a novel protein motif for nucleic acid recognition. Trends in Biochemical Sciences 12:464–469
    [Google Scholar]
  13. Kondo K., Bando H., Tsurudome M., Kawano M., Nishio M., Ito Y. 1990; Sequence analysis of the phosphoprotein (P) genes of human parainfluenza type 4A and 4B viruses and RNA editing at transcript of the P genes: the number of G residues added is imprecise. Virology 178:321–326
    [Google Scholar]
  14. Landschulz W. H., Johnson P. F., McKnight S. L. 1988; The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 240:1759–1764
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Matsouka Y., Curran J., Pelet T., Kolakofsky D., Ray R., Compans R. W. 1991; The P gene of human parainfluenza virus type 1 encodes P and C proteins but not a cysteine rich V protein. Journal of Virology 65:3406–3410
    [Google Scholar]
  17. Moreno-López J., Correa-Girón P., Martinez A., Ericsson A. 1986; Characterization of a paramyxovirus isolated from the brain of a piglet in Mexico. Archives of Virology 91:221–231
    [Google Scholar]
  18. Ohgimoto S., Bando H., Kawano M., Okamoto K., Kondo K., Tsurudome M., Nishio M., Ito Y. 1990; Sequence analysis of P gene of human parainfluenza type 2 virus: P and cysteine-rich proteins are translated by two mRNAs that differ by two nontemplated G residues. Virology 177:116–123
    [Google Scholar]
  19. Paterson R. A., Lamb R. A. 1990; RNA editing by G-nucleotide insertion in mumps virus P-gene mRNA transcripts. Journal of Virology 64:4137–4145
    [Google Scholar]
  20. Pelet T., Curran J., Kolakofsky D. 1991; The P gene of bovine parainfluenza virus 3 expresses all three reading frames from a single mRNA editing site. EMBO Journal 10:443–448
    [Google Scholar]
  21. Ryan K. W., Portner A. 1990; Separate domains of Sendai virus P protein are required for binding to viral nucleocapsids. Virology 174:515–521
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  23. Southern J. A., Precious B., Randall R. E. 1990; Two nontemplated nucleotide additions are required to generate the P mRNA of parainfluenza type 2 since the RNA genome encodes protein V. Virology 177:388–390
    [Google Scholar]
  24. Stephano H. A., Gay G. M., Ramirez T. C. 1988; Encephalomyelitis, reproductive failure and corneal opacity (blue eyes) in pigs, associated with a paramyxovirus infection. Veterinary Record 122:6–10
    [Google Scholar]
  25. Sundqvist A., Berg M., Hernandez-Jauregui P., Linné T., Moreno-López J. 1990; The structural proteins of a porcine paramyxovirus (LPMV). Journal of General Virology 71:609–613
    [Google Scholar]
  26. Sundqvist A., Berg M., Moreno-López J., Linné T. 1992; The hemagglutinin-neuraminidase gene of the porcine paramyxovirus LPMV: comparison with other paramyxoviruses revealed the closest relationship to simian virus 5 and mumps virus. Archives of Virology 122:331–340
    [Google Scholar]
  27. Thomas S. M., Lamb R. A., Paterson R. A. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  28. Vidal S., Curran J., Orvell C., Kolakofsky D. 1988; Mapping of monoclonal antibodies to the Sendai virus P protein and the location of its phosphates. Journal of Virology 62:2200–2203
    [Google Scholar]
  29. Vidal S., Curran J., Kolakofsky D. 1990; Editing of the Sendai virus P/C mRNA by G insertion occurs during mRNA synthesis via a virus-encoded activity. Journal of Virology 64:239–246
    [Google Scholar]
  30. Yamada H., Hayata S., Omata-Yamada T., Taira H., Mizumoto K., Iwasaki K. 1990; Association of the Sendai virus C protein with nucleocapsids. Archives of Virology 113:245–253
    [Google Scholar]
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