The nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons Free

Abstract

The genomic region of the multiple nucleocapsid nuclear polyhedrosis virus (AgMNPV) strain 2D encoding the polyhedrin gene was cloned and mapped, and a 2085 bp I-I fragment containing the gene was sequenced. The polyhedrin polypeptide of the parental isolate AgMNPV was manually sequenced, and the amino acid sequence obtained agreed with that deduced from the DNA coding region sequence. AgMNPV and MNPV (OpMNPV) are similar in terms of promoter structure and polyhedrin primary sequence, and the polyhedrin gene of both viruses is transcribed in the anti-clockwise direction in relation to their physical maps. The region upstream from the polyhedrin gene of AgMNPV, OpMNPV, NPV and MNPV (AcMNPV) was compared and this showed that the open reading frame (ORF) common to all four viruses (ORF 5) has sequence homology with the AcMNPV 25K gene. The sequences between ORF 5 and the polyhedrin gene were found to be variable among the polyhedrin gene loci compared. Additionally, conserved elements in the promoters of the very late genes encoding polyhedrin and granulin, and those encoding two p10 proteins were found to share sequence homology and positional similarity with consensus regions in the conserved boxes A and C, responsible for binding transcription factors to eukaryotic 5S ribosomal RNA genes, and to box C of tRNA genes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-5-1049
1992-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/5/JV0730051049.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-5-1049&mimeType=html&fmt=ahah

References

  1. Akiyoshi D., Chakerian R., Rohrmann G. F., Nesson M. H., Beaudreau G. S. 1985; Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus. Virology 141:328–332
    [Google Scholar]
  2. Beames B., Summers M. D. 1989; Location and nucleotide sequence of the 25K protein missing from baculovirus few polyhedra (FP) mutants. Virology 168:344–353
    [Google Scholar]
  3. Berger S. L., Kimmel A. R. 1987; Guide to molecular cloning techniques. Methods in Enyzmology 152:94–110
    [Google Scholar]
  4. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 53:127–155
    [Google Scholar]
  5. Brown F. 1986; The classification and nomenclature of viruses: summary of results of the International Committee on Taxonomy of Viruses in Sendai, September (1984). Intervirology 25:141–143
    [Google Scholar]
  6. Cameron I. R., Possee R. D. 1989; Conservation of polyhedrin gene promoter function between Autographa californica and Mamestra brassicae nuclear polyhedrosis viruses. Virus Research 12:183–200
    [Google Scholar]
  7. Chakerian R., Rohrmann G. F., Nesson M. H., Leisy D. J., Beaudreau G. S. 1985; The nucleotide sequence of the Pieris brassicae granulosis virus granulin gene. Journal of General Virology 66:1263–1269
    [Google Scholar]
  8. Chang J. Y. 1986; Manual sequence analysis of polypeptides using 4-dimethyl-aminoazobenzene-4′-isothiocyanate. In Practical Protein Chemistry - A Handbook pp 427–433 Edited by Darbre A. New York: John Wiley;
    [Google Scholar]
  9. Chang M. T., Lanner-Herrera C., Fikes M. 1989; Nucleotide sequence of Lymantria dispar nuclear polyhedrosis virus polyhedrin gene. Journal of Invertebrate Pathology 53:241–246
    [Google Scholar]
  10. Chen E. Y., Seeburg P. K. 1985; Supercoil sequencing: a fast simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  11. Davis L. G., Dibner M. D., Battey J. F. 1986 Basic Methods in Molecular Biology Amsterdam: Elsevier;
    [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  13. Dillon L. S. 1987; The 5S ribosomal and other small RNAs. In The Gene:Its Structure, Function and Evolution pp 93–143 New York: Plenum Press;
    [Google Scholar]
  14. Gearing K. L., Possee R. D. 1990; Functional analysis of a 603 nucleotide open reading frame upstream of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Journal of General Virology 71:251–262
    [Google Scholar]
  15. Gonzalez M. A., Smith G. E., Summers M. D. 1989; Insertion of the SfMNPV polyhedrin gene into an AcMNPV polyhedrin deletion mutant during viral infection. Virology 170:160–175
    [Google Scholar]
  16. Granados R. R., Williams K. A. 1986; In vivo infection and replication of baculovirus. In The Biology of Baculoviruses vol 1 pp 89–108 Edited by Federici B., Granados R. Boca Raton: CRC Press;
    [Google Scholar]
  17. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  18. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni. Nature, London 226:466–467
    [Google Scholar]
  19. Hink W. F., Vail P. V. 1973; A plaque assay for titration of alfafa looper nuclear polyhedrosis virus in cabbage looper (TN-368) cell line. Journal of Invertebrate Pathology 22:168–174
    [Google Scholar]
  20. Hooft van Iddekinge B. J. L., Smith G. E., Summers M. D. 1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
    [Google Scholar]
  21. Howard S. C., Ayres M. D., Possee R. D. 1986; Mapping of the 5′ and 3′ ends of Autographa californica nuclear polyhedrosis virus polyhedrin messenger RNA. Virus Research 5:109–120
    [Google Scholar]
  22. Huh N. E., Weaver R. F. 1990; Identifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus. Journal of General Virology 71:195–201
    [Google Scholar]
  23. Iatrou K., Ito K., Witkiewicz H. J. 1985; Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus. Journal of Virology 54:436–445
    [Google Scholar]
  24. Johnson D. W., Maruniak J. E. 1989; Physical map of Anticarsia gemmatalis nuclear polyhedrosis virus (AgMNPV-2) DNA. Journal of General Virology 70:1877–1883
    [Google Scholar]
  25. Kozak M. 1983; Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Research 12:857–872
    [Google Scholar]
  26. Kuzio J., Rohel D. Z., Curry C. J., Krebs A., Carstens E. B., Faulkner P. 1984; Nucleotide sequence of the p10 polypeptide gene of Autographa californica nuclear polyhedrosis virus. Virology 139:414–418
    [Google Scholar]
  27. Lassar A. B., Martin P. L., Roeder R. G. 1983; Transcription of class III genes: formation of preinitiation complexes. Science 222:740–748
    [Google Scholar]
  28. Leisy D. J., Rohrmann G., Beaudreau G. S. 1986a; The nucleotide sequence of the polyhedrin gene region from the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata. Virology 153:280–288
    [Google Scholar]
  29. Leisy D., Nesson M., Pearson M., Rohrmann G., Beaudreau G. 1986b; Location and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus polyhedrin gene. Journal of General Virology 67:1073–1079
    [Google Scholar]
  30. Leisy D. J., Rohrmann G. F., Nesson M. H., Beaudreau G. S. 1986c; Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene. Virology 153:157–167
    [Google Scholar]
  31. Lewin B. 1990; Building the transcription complex. In Genes IV pp 574–576 Cambridge, Massachusetts: Oxford University Press and Cell Press;
    [Google Scholar]
  32. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. BiolTechnology 6:47–55
    [Google Scholar]
  33. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  34. Maruniak J. E. 1989; Molecular biology of Anticarsia gemmatalis baculovirus. Membrias do Instituto Oswaldo Cruz 84:107–111
    [Google Scholar]
  35. Maruniak J. E., Brown S. E., Knudson D. L. 1984; Physical maps of SfMNPV baculovirus DNA and its genomic variants. Virology 136:221–234
    [Google Scholar]
  36. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirement for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  37. Moscardi F. 1989; Use of viruses for pest control in Brazil: the case of the nuclear polyhedrosis virus of the soybean caterpillar, Anticarsia gemmatalis. Memórias do Instituto Oswaldo Cruz 84:51–56
    [Google Scholar]
  38. Moscardi F., Corrêa Ferreira B. S. 1985; Biological control of soybean caterpillars. In World Research Conference III Proceedings pp 703–711 Edited by Shibles R. Boulder & London: Westview Press;
    [Google Scholar]
  39. Oakey R., Cameron I. R., Davis B., Davis E., Possee R. D. 1989; Analysis of transcription initiation in the Panolis fiammea nuclear polyhedrosis virus polyhedrin gene. Journal of General Virology 70:769–775
    [Google Scholar]
  40. Ohlendorf D. H., Matthews B. W. 1983; Structural studies of protein-nucleic acid interactions. Annual Review of Biophysics and Bioengineering 12:259–284
    [Google Scholar]
  41. Ooi B. G., Miller L. K. 1990; Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream. Journal of Virology 64:3126–3129
    [Google Scholar]
  42. Ooi B. G., Rankin C., Miller L. K. 1989; Downstream, sequences augment transcription from the essential initiation site of a baculovirus polyhedrin gene. Journal of Molecular Biology 210721–736
    [Google Scholar]
  43. Orcutt B. C., George D. G., Johnson G. C., Barker W. C. 1984; NAQ-Nucleic acid sequence query, a tutorial. In User’s Guide to the Software System of the Protein Identification Resource Edited by Gilbert W. A. Washington, D. C.: National Biomedical Research Foundation;
    [Google Scholar]
  44. Possee R. D., Sun T. P., Howard S. C., Ayres M. D., Hill-Perkins M., Gearing K. L. 1991; Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 Kbp Eco RI-I and -R (polyhedrin gene) region. Virology 185:229–241
    [Google Scholar]
  45. Rankin C., Ooi B. G., Miller L. K. 1988; Eight base pairs encompassing the transcriptional start point are the major determinant for baculovirus polyhedrin gene expression. Gene 70:39–49
    [Google Scholar]
  46. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  47. Saenger W. 1984; Polymorphism of DNA versus structural conservatism of RNA: classification of A-, B- and Z-type double helices. In Principles of Nucleic Acid Structure. Springer Advanced Texts in Chemistry pp 221–241 Edited by Cantor C. New York: Springer Verlag;
    [Google Scholar]
  48. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  49. Sieburth P. J., Maruniak J. E. 1988; Growth characteristics of a continuous cell line from the velvetbean caterpillar, Anticarsia gemmatalis Hiibner (Lepidoptera: Noctuidae). In Vitro Cellular Developmental Biology 24:195–198
    [Google Scholar]
  50. Smith G. E., Summers M. D. 1982; DNA homology among subgroup A, B, and C baculoviruses. Virology 123:393–406
    [Google Scholar]
  51. Smith G. E., Summers M. D., Fraser M. J. 1983; Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3:2156–2165
    [Google Scholar]
  52. Southern E. M. 1979; Measurement of DNA length by gel electrophoresis. Analytical Biochemistry 100:319–323
    [Google Scholar]
  53. Van Strien E. A., Naaktgeboren J. D., Zuidema D., Vlak J. M. 1990; Physical map of the Spodoptera exigua nuclear polyhedrosis virus genome: localization and sequencing of the polyhedrin gene. Abstracts of the VIII International Congress of Virology, Berlin p 153
    [Google Scholar]
  54. Vaughn J. L., Goodwin R. H., Thompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  55. Weyer U., Possee R. D. 1989; Analysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene. Journal of General Virology 70:203–208
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-5-1049
Loading
/content/journal/jgv/10.1099/0022-1317-73-5-1049
Loading

Data & Media loading...

Most cited Most Cited RSS feed