RT Journal Article SR Electronic(1) A1 Li, Peng A1 Kuiper, Lara J. A1 Stephenson, Alice J. A1 Burrell, Christopher J.YR 1992 T1 De novo reverse transcription is a crucial event in cell-to-cell transmission of human immunodeficiency virus JF Journal of General Virology, VO 73 IS 4 SP 955 OP 959 DO https://doi.org/10.1099/0022-1317-73-4-955 PB Microbiology Society, SN 1465-2099, AB The proposal that replication of human immunodeficiency virus type 1 (HIV-1), mediated by cell-to-cell transmission of the virus, might bypass de novo reverse transcription was tested by using one-step cell-to-cell and cell-free virus infection systems. Two well characterized reverse transcriptase (RT) inhibitors, azidothymidine at 20 µm and phosphonoformic acid at 100 µg/ml, blocked HIV replication completely following both cell-free virus and cell-to-cell transmission infection, as determined from the kinetics of unintegrated viral DNA synthesis and supernatant RT production after virus infection. Our results confirm that de novo reverse transcription is a crucial and mandatory event in HIV-1 replication following cell-to-cell transmission of the virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-4-955