1887

Abstract

The proposal that replication of human immunodeficiency virus type 1 (HIV-1), mediated by cell-to-cell transmission of the virus, might bypass reverse transcription was tested by using one-step cell-to-cell and cell-free virus infection systems. Two well characterized reverse transcriptase (RT) inhibitors, azidothymidine at 20 µ and phosphonoformic acid at 100 µg/ml, blocked HIV replication completely following both cell-free virus and cell-to-cell transmission infection, as determined from the kinetics of unintegrated viral DNA synthesis and supernatant RT production after virus infection. Our results confirm that reverse transcription is a crucial and mandatory event in HIV-1 replication following cell-to-cell transmission of the virus.

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/content/journal/jgv/10.1099/0022-1317-73-4-955
1992-04-01
2022-01-18
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References

  1. Bukrinsky M. I., Stanwick T. L., Dempsey M. P., Stevenson M. 1991; Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection. Science 254:423–427
    [Google Scholar]
  2. De Clercq E. 1989; New acquisitions in the development of anti-HIV agents. Antiviral Research 12:1–19
    [Google Scholar]
  3. Dietzschold B., Wiktor T. J., Trojanowski J. Q., MacFarlan R. I., Wunner W. H., Torres-Anjel M. J., Koprowski H. 1985; Differences in cell-to-cell spread of pathogenic and apathogenic rabies virus in vivo and in vitro . Journal of Virology 56:12–18
    [Google Scholar]
  4. Gupta P., Balachandran R., Ho M., Enrico A., Rinaldo C. 1989; Cell-to-cell transmission of human immunodeficiency virus type 1 in the presence of azidothymidine and neutralizing antibody. Journal of Virology 63:2361–2365
    [Google Scholar]
  5. Hahn B. H., Shaw G. M., Arya S. K., Popovic M., Gallo R. C., Wong-Staal F. 1984; Molecular cloning and characterization of the HTLV-III virus associated with AIDS. Nature, London 312:166–169
    [Google Scholar]
  6. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  7. Hoffman A. D., Banapour B., Levy J. A. 1985; Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions. Virology 147:326–335
    [Google Scholar]
  8. Hooks J. J., Burns W., Hayashi K., Geis S., Notkins A. L. 1976; Viral spread in the presence of neutralizing antibody: mechanisms of persistence in foamy virus infection. Infection and Immunity 14:1172–1178
    [Google Scholar]
  9. Li P., Burrell C. J. 1991; Synthesis of human immunodeficiency virus DNA in a cell-to-cell transmission model. AIDS Research and Human Retroviruses (in press)
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Myers G., Rabson A. B., Berzofsky J. A., Smith T. F., Wong-Staal F. 1990 Human Retroviruses and AIDS. Los Alamos New Mexico: Los Alamos National Laboratory;
    [Google Scholar]
  12. Nakashima H., Matsui T., Harada S., Kobayashi N., Matsuda A., Ueda T., Yamamoto N. 1986; Inhibition of replication and cytopathic effect of human T cell lymphotropic virus type III/ lymphadenopathy-associated virus by 3′-azido-3′-deoxythymidine in vitro . Antimicrobial Agents and Chemotherapy 30:933–937
    [Google Scholar]
  13. Öberg B. 1983; Antiviral effects of phosphonoformate. Pharmacology and Therapeutics 19:387–415
    [Google Scholar]
  14. Pietroboni G. R., Harnett G. B., Bucens M. R. 1989; Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro . Journal of Virological Methods 24:85–90
    [Google Scholar]
  15. Smith M. S., Brian E. L., Pagano J. S. 1987; Resumption of virus production after human immunodeficiency virus infection of T lymphocytes in the presence of azidothymidine. Journal of Virology 61:3769–3773
    [Google Scholar]
  16. Wondrak E. M., Lower J., Kurth R. 1988; Inhibition of HIV-1 RNA-dependent DNA polymerase and cellular DNA polymerases alpha, beta and gamma by phosphonoformic acid and other drugs. Journal of Antimicrobial Chemotherapy 21:151–161
    [Google Scholar]
  17. Yarchoan R., Mitsuya H., Broder S. 1990; Immunologic issues in anti-retroviral therapy. Immunology Today 11:327–333
    [Google Scholar]
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